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Sireesha, D.
- Development and Validation of Bioanalytical Method for the Estimation of Carisoprodol in Human Plasma using LC-MS/MS
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Authors
Affiliations
1 College of Technology, Osmania University, Hyderabad, Telangana, IN
2 School of Pharmacy, Aanurag Group of Institutions, Ghatkesar, Ranga Reddy, IN
1 College of Technology, Osmania University, Hyderabad, Telangana, IN
2 School of Pharmacy, Aanurag Group of Institutions, Ghatkesar, Ranga Reddy, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 5, No 4 (2015), Pagination: 181-186Abstract
Simple, rapid and highly sensitive liquid chromatography- tandem mass spectrometric (LC-MS/MS) assay method was developed for the determination of Carisoprodol in human plasma. Carisoprodol methyl D3 was used as an internal standard (IS). The method employed 250μl of human plasma for sample processing by a simple Liquid Liquid Extraction (LLE) technique. The processed samples were chromatographer on a Phenyl column by using a mixture of 10mM Ammonium format - Acetonitrile (15:85,v/v) as the mobile phase at a flow rate of 1.2ml/min. The calibration curve obtained was linear over the concentration range of 25-3000ng/ml with r2 > 0.99. Method validation was performed as per the FDA guidelines and the results met the acceptance criteria. The Selected Ion Monitoring (SIM) mode was used for quantification of ion transitions at m/z 261.3/176.1 and 264.4/179.2 for the analyte and the IS respectively. A run time of 2.0 min was used which made it possible to analyze more than 400 plasma samples per day, thus increasing the productivity.Keywords
Carisoprodol, LC-MS/MS, Bionalytical Method, Liquid-Liquid Extraction, Internal Standard.- Development and Validation of UV Spectrophotometric Method for the Simultaneous Estimation of Rosuvastatin and Ezetimibe in Pharmaceutical Dosage Form
Abstract Views :230 |
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Authors
Affiliations
1 Anurag Group of Institutions (Formerly Lalitha College of Pharmacy), Venkatapur, Ghatkesar, Medchal, Telangana-500088, IN
1 Anurag Group of Institutions (Formerly Lalitha College of Pharmacy), Venkatapur, Ghatkesar, Medchal, Telangana-500088, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 7, No 3 (2017), Pagination: 135-140Abstract
A simple, accurate, precise and economical spectrophotometric method was developed and validated for simultaneous estimation of Rosuvastatin (RST) and Ezetimibe (EZE) in combined dosage form. In simultaneous equation method, Rosuvastatin and Ezetimibe were quantified using their absorptivity values at selected wavelengths, viz; 223nm and 229nm respectively. Distilled water was used as the solvent. Different analytical performance parameters such as linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ) was determined according to ICH guidelines. The linearity range was found to be 4-32 μg/ml for both the drugs. LOD and LOQ for Rosuvastatin were found to be 1.1μg/ml and 3.5μg/ml, for Ezetimibe 1.1μg/ml and 3.5μg/ml respectively.Keywords
Rosuvastatin, Ezetimibe, Spectrophotometric Method, Method Development, Validation.References
- https://www.drugbank.ca/drugs/DB01098
- https://pubchem.ncbi.nlm.nih.gov/compound/Rosuvastatin
- https://www.drugbank.ca/drugs/DB00973
- https://pubchem.ncbi.nlm.nih.gov/compound/Ezetimibe
- Vishal V. Rajkondwar, Pramila Maini and Monika Vishwakarma; Characterization and method development for estimation and validation of Rosuvastatin Calcium by UV – visible spectrophotometry; International Journal of Theoretical and Applied Sciences; 2009; 1(1); pg.no. 48-53.
- Alka Gupta, P. Mishra and K. Shah; Simple UV Spectrophotometric Determination of Rosuvastatin Calcium in Pure Form and in Pharmaceutical Formulations; ECJHAO E-Journal of Chemistry; 2009; 6(1); pg.no. 89-92.
- Gajjar Anuradha K and Shah Vishal D; Simultaneous UV Spectrophotometric Estimation of Rosuvastatin and Ezetimibe in their combined dosage forms; International Journal of Pharmacy and Pharmaceutical Sciences; 2010; 2(1); pg.no. 131-138.
- Anuradha K. Gajjar and Vishal D. Shah; Development and Validation of a Stability-Indicating Reversed-Phase HPLC method for Simultaneous Estimation of Rosuvastatin and Ezetimibe for their Combination Dosage Forms; Eurasian Journal of Analytical Chemistry; 2010; 5(3); pg.no. 280-298.
- Varghese, Sushell John, Ravi, Thengungal Kochupappy; Determination of Rosuvastatin and Ezetimibe in a Combined Tablet Dosage form using High-Performance Column Liquid Chromatography and High-Performance Thin-Layer Chromatography; Journal of AOAC International; 2010; 93(6); pg.no. 1222-1227.
- Hasumati A. Raj, Sadhana J. Rajput, Jayant B. Dave and Chaggan N. Patel; Development and Validation of Two Chromatographic Stability-Indicating Methods for determination of Rosuvastatin in pure form and Pharmaceutical preparation; International Journal of Chem Tech Research; 2009; 1(3); pg.no.677-689.