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D. Prashanth , G. Balaji , M. K. Asha , J. Biju , S. Yogisha , A. Amit

Abstract

Objective: To determine the stability of the anti-microbial activity of Wisprec. Materials and methods: Various batches of the anti-microbial cream Wisprec were tested against six microorganisms using agar dilution technique. Result: All the batches tested were stable with respect to their anti-microbial activity. Conclusion: The result suggests that the anti-microbial activity of Wisprec remains intact upto 3 Januarys from the date of manufacture.

Keywords

Anti-microbial Activity, Wisprec, Stability Studies

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D. S. Samiulla , D. Prashanth , A. Amit , B. V. Venkataraman

Abstract

Four successive extracts (petroleum ether, chloroform, methanol and water) of P. granatum fruit rind were tested for in-vitro trypsin inhibitory activity using commercially available enzyme (Porcine pancreatic type II trypsin) and substrate (Benzoyl-D,L-arginine-4-nitroanilide) and the activity was compared with standard trypsin inhibitor from hen egg white (ovomucoid) . The methanolic extract was found to be most active and the activity was comparable to that of the reference standard.

Keywords

Punica Granatum, Ovomucoid, Trypsin Inhibitor, Proteases

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D. Prashanth , A. Amit , S. Yogisha , R. Padmaja

Abstract

Objective: To demonstrate biological standardization of selected herbal extracts using rapid and convenient bioassays. Methods Boswellia serrata alcoholic extract (resin), Emblica officinalis water extract (fruit) and Salacia oblonga 80%alcoholic extract (ischolar_main) were tested with reference to lipoxygenase inhibition, anti-oxidant and α-glucosidase inhibitory activity respectively. Brine Shrimp lethality assay (BSLA) was used for standardization of Bacopa monnieri alcoholic extract (leaves). Piper longum extracts with varying concentration of piperine were also tested in BSLA. To evaluate the use of BSLA in detecting adulteration, samples of Alpinia galanga deliberately adulterated with Acorus calamus rhizomes were tested. Results : IC₅₀ limits were established for extracts of B.serrata, E.officinalis & S. oblanga. Limits of acceptance were also achieved for B. monnieri in terms of LC₅₀ values. BSLA was also capable of detecting the difference in Piper longum extracts, which were varying in Piperine content, the active constituent of Piper longum. BSLA was also sensitive to detect change in bioactivity of Alpinia galanga (rhizome) samples, which were deliberately adulterated with Acorus calamus rhizomes. Conclusion : The use of bioassays is suggested for effective quality control of herbal products.

Keywords

Bioassays, Biological Standardization, Herbal Drugs, Quality Control

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