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D. P. Kuldhar ¹, S. L. Badgujar ¹, Utpal Dey ¹

Affiliations
1 Department of Plant Pathology, College of Agriculture, Marathwada Krishi Vidyapeeth, Parbhani M.S., IN

Abstract

Field experiment has been carried during Rabi, 2009, to find out pea varieties, germplasm lines and genotypes against pea wilt incited by Fusarium oxysporum f.sp. pisi. Among the pea verieties tested, Arkel was found most susceptible with significantly highest mean wilt incidence (22.66%), and this was followed by Latur local-1 (29 %) and Latur Local-2 (28%). Soldier was found moderately susceptible with mean wilt incidence of 19 per cent. Thus, pea variety Soldier may be preferred for sowing during first fortnight of October, so as to minimize the yield losses due to wilt incidence.

Keywords

Pea Wilt, Screening, F. Oxysporum F.sp. Pisi

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D. P. Kuldhar ¹, S. L. Badgujar ¹, Utpal Dey ¹

Affiliations
1 Department of Plant Pathology, College of Agriculture, Marathwada Krishi Vidyapeeth, Parbhani, M.S., IN

Abstract

An experiment has been conducted during Rabi season, 2009, to evaluate the effect of different soil types viz., black, red, sandy, sandy loam, and clay soil on incidence of pea wilt incited by Fusarium oxysporum f.sp. pisi. Results revealed that the disease was significantly highest in black soil (96.40%) followed by clay soil (58.00%). While, least wilt incidence was noticed on red soil (29.20%). It was recorded that the disease incidence varied from locality depending upon cropping pattern, environmental condition and inoculum buildup.

Keywords

Pea, Wilt, Fusarium Oxysporum F.sp. Pisi, Soil Types

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M. R. Taware ¹, V. M. Gholve ¹, S. S. Wagh ¹, D. P. Kuldhar ¹, D. V. Pawar ¹, A. A. Chavan ¹

Affiliations
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Prabhani (M.S.), IN

Abstract

Eight culture media, eight temperature levels, nine pH levels, seven carbon and six nitrogen sources tested exhibited better growth of Alternaria carthami. Results revealed that Potato dextrose agar gave significantly highest growth (90.00 mm), followed by Potato malt agar (84.16 mm) with excellent sporulation. Temperature levels indicated that highest mean mycelial growth (85.66 mm) was recorded at 30°C followed by 25°C (83.83 mm) and 20°C (66.33 mm). However, maximum mean mycelial growth (85.83 mm) was recorded at pH 6.5 with excellent sporulation, followed by at pH 6 (82.00 mm) and pH 7 (70.33 mm) with excellent and good sporulation, respectively. The carbon sources exhibited varied radial mycelial growth and sporulation of the test pathogen. However, highest radial mycelial growth (86.00 mm) and excellent sporulation was recorded on glucose, followed by on maltose (82.83 mm) and starch (80.33 mm) with excellent sporulation. Nitrogen sources resulted highest radial mycelial growth (82.55 mm) and excellent sporulation on potassium nitrate, followed by on peptone (75.83 mm) with good sporulation. Least radial mycelial growth (19.00 mm) was recorded on urea with poor sporulation.

Keywords

Alternaria Carthami, Mycelial Growth, Carbon And Nitrogen Sources

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A. A. Wanggikar ¹, S. S. Wagh ², D. P. Kuldhar ², D. V. Pawar ²

Affiliations
1 Department of Plant Pathology, College of Agriculture, Latur (M. S.), IN
2 Department of Plant Pathology, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Prabhani (m.s.), IN
3 Department of Plant Pathology, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Prabhani (M.S.), IN

Abstract

A study was conducted in the of Department Plant Pathology, College of Agriculture, Latur, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani, Maharashtra, India, during 2011 to control Alternaria porri causing Alternaria blight of onion with fungicides, botanical and bio-agents. Among nine treatments, six fungicides (@ 100, 200, 250 and 500 ppm concentrations), one plant extract and two bioagents (@ 500 ppm) were evaluated in vitro in vivo and were found effective against A. porri and recorded significant inhibition of the test pathogen over untreated control. However, in vitro result revealed that in hexaconozole cent per cent (100.00 %) inhibition was observed, followed by difenoconazole (83.91 %), mancozeb (63.58%), P. florescence (58.94 %) and T. viride (54.45%). The minimum per cent inhibition was observed in chlorothalonil (31.40 %) followed by plant extract NSKE (43.92 %), copper oxychloride (46.87 %) and carbandazim (47.11 %). In vivo results revealed that hexaconozole (0.1%) was found most effective and recorded significantly least mean disease incidence (6.03 %) and intensity (13.33 %) with corresponding significantly increased bulb yield (438.00 q/ha) followed by mancozeb (@ 0.2%) and copper oxychloride (0.25%) which recorded significantly mean disease incidence of 6.83 and 8.53 per cent and intensity, 15.00 and 20.00 per cent, respectively and gave correspondingly bulb yield, respectively of 375.00 and 429.00 q/ha. The botanical tested, A. indica (@ 5%) was found antifungal against A. porri and recorded significantly disease incidence (7.96 %) and intensity (27.00 %), and gave the bulb yield (290.00 q/ha). Both fungal and bacterial antagonists tested were found not so effective to reduce incidence and intensity, attempt increased the bulb yield over unsprayed control.

Keywords

Onion, Alternaria Porri, Purple Blotch, In Vitro And in Vivo Evaluation

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Amreen Begum ¹, M. S. Dadke ¹, S. S. Wagh ², D. P. Kuldhar ², D.V. Pawar ², A. A. Chavan ², D. S. Thaware ²

Affiliations
1 Department of Plant Pathology, College of Agriculture, Latur (M.S.), IN
2 Department of Plant Pathology, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M. S.), IN

Abstract

Stem rot caused by Sclerotium rolfsii Sacc. has been observed to cause rapid mortality in chilli plantations. Among eight fungicides and eight botanicals tested in vitro against S. rolfsii, the result revealed that maximum (100%) inhibition was observed in carboxin, propiconazole, hexaconazole, difenconazole and carbendazim at all three concentrations viz., 500, 1000 and 1500 ppm followed by captan (79.30, 82.76 and 85.23%) and triadimenfon (49.13, 60.23 and 65.33%) over control. Minimum per cent of inhibition was observed in the plates poisoned with copper oxychloride (47.26, 51.63 and 54.40%), respectively at all three concentrations. Among botanicals, at 5 and 10 per cent concentrations, significantly highest average inhibition was recorded with neem (74.81%), followed by tulsi (67.10%) and nirgudi (65.81%). Significantly least average inhibition was recorded with sorghum (47.23 %). The rest of the botanicals recorded more than 50.00 per cent average inhibition of mycelial growth over untreated control (00.00%).

Keywords

Chilli, S. Rolfsii, Stem Rot, in Vitro Evaluation, Fungicides, Botanicals

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S. R. Jukte ¹, S. L. Badgujar ¹, A. P. Suryawanshi ¹, Utpal Dey ¹, D. P. Kuldhar ¹

Affiliations
1 Department of Plant Pathology, V.N. Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN

Abstract

Damping off diseases in okra is an economically most important and destructive disease of okra. The main characteristic symptom of the disease is pre- emergence damping off i.e. rotting of the seeds and seedlings before actual emergence from the soil and postemergence damping off which is severe when the seedlings are in cotyledonous stage. The infected tissues become soft and water soaked resulting in toppling over of the entire plant on the soil surface. The test pathogen (Pythium aphanidermatum) was isolated successfully on the basal culture medium Potato dextrose agar, from the seedlings showing typical symptoms of damping off. The pathogen produced nonseptate, well branched, colourless to white mycelium, lobed sporangia on indeterminate sporangiophores, and formation of resting spore (oospore) when observed under the microscope. Pathogenicity of P. aphanidermatum was proved by sick soil method in pot culture, sowing okra cv. PARBHANI KRANTI under screen house condition and by water agar method. The pathogen was reisolated on PDA from artificially diseased okra seedling, and compared its cultural and morphological characteristics with the original fungus isolated from the naturally damping off diseased okra plant.

Keywords

Damping Off, Isolation, Okra, Pythium spp., Pathogenicity, Symptomatology.

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