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Marimuthu, Chandramohan
- Miniaturized Screening and Isolation of Phospholipase A2 Rhizopus by Using Egg Yolk Substrate
Abstract Views :879 |
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Authors
Chandramohan Marimuthu
1,
Chandrakala Amrose
2,
Jenifier Abel
3,
Mohanapriya Muniappan
1,
Kasthuri Gnanasekaran
1
Affiliations
1 Microcore Research Laboratories India Pvt Ltd R&D Erode, Tamil Nadu
2 Lecturer Dept of Zoology Shri Vasavi College Erode, Tamil Nadu
3 Research Scholar Dept of Zoology Shri Vasavi College Erode, Tamil Nadu
1 Microcore Research Laboratories India Pvt Ltd R&D Erode, Tamil Nadu
2 Lecturer Dept of Zoology Shri Vasavi College Erode, Tamil Nadu
3 Research Scholar Dept of Zoology Shri Vasavi College Erode, Tamil Nadu
Source
International Journal of Applied Biotechnology and Biochemistry, Vol 3, No 1 (2013), Pagination: 83-96Abstract
The study describes miniaturized screening and isolation methods for phospholipase A2 producing fungi from egg processing environment. The 15 different samples from various locations of egg processing industry was collected and cultured. The primary screening media contains antibacterial antibiotics for selective isolation of fungi and also Octadecyl rhodamine serves as lipophilic and selective agent to indicate and deduct the lyses of lipid. Plates are Incubated at 25º C for 4 to 6 days resulted single positive colony among 139 colonies. Primary screening was examined by natural lyses or inhibition of Octadecyl rhodamine diffusion. The inhibition indicates the possible phospholipase activity. Single colony was selected for dye lyses and further tested for secondary screening on shake flask based submerged fermentation. The cell free supernatant are tested with 9.4 ml of egg yolk substrate prepared with 0.6ml 3M CaCl2 (de-ionized water) and resulted rapid hydrolysis and pH reduction and further tested on lab Koji Solid state fermentation. The study has resulted phospholipase A2 producing Rhizopus labeled MRL/IS 85 and effected 110u/ml in SMF and 300u/g in SSF with wheat bran substrate pretreated with caclium chloride as catalyst and also fed with phospholipid inducers.Keywords
Phospholipase A-2, Lysolecithin And Phosphatidylcholine 2-AcylhydrolaseReferences
- Hanahan, D.J., Brockerhoff, H. and Barron, E.J. The site of attack of phospholipase (lecithinase) A on lecithin: a re-evaluation. Position of fatty acids on lecithins and triglycerides. J. Biol. Chem. 235 (1960) 1917-1923.
- Moore, J.H. and Williams, D.L. Some observations on the specificity of phospholipase A. Biochim. Biophys. Acta 84 (1964) 41-54.
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- Validation Report No. 00622.UK1, the validation of the phospholipase A2 egg yolk method.
- Optimizations PLA2 egg yolk assay ARS00622.NL2, ALS reference: 2000-086
- Effects on Oral Supplementation of Nutraceuticals formulations - Trichovitals® in young adults for healthy maintenance of hair growth
Abstract Views :143 |
PDF Views:0
Authors
Affiliations
1 Microcore Research Laboratories India Pvt Ltd., 9th km, 30 Feet Road, 204 - A Poondurai Main Road, Checkmedu, Erode - 638115, Tamil Nadu, IN
1 Microcore Research Laboratories India Pvt Ltd., 9th km, 30 Feet Road, 204 - A Poondurai Main Road, Checkmedu, Erode - 638115, Tamil Nadu, IN
Source
Research Journal of Topical and Cosmetic Sciences, Vol 13, No 1 (2022), Pagination: 1-8Abstract
Nutrition is a major factor in affecting the health and appearance of hair. In addition to how one eats dietary supplementation plays very critical role in overall maintenance of healthy hair. Precisely Trichovitals® contains Natural Egg Shell Membrane as source of Collagen type (I, III, V, X) and Elastin, Ellagic acid an extract from Pomegranate red pigment, Sabbery (10% beta- glucogallin) an extract of Amla fruit, Biotin (Water soluble vitamin), Tetra hydro curcumin (Derivate of Curcumin) and Murraya koenigii (an extract of the curry leaf as natural source of the iron). With respected to the prior art data each ingredient has several independent clinical studies to support the growth of the healthy hair and influences strong impact on hair thickness, reduction in hair breakage and improvement in hair growth. This study leads to evaluate the impacts of synergy of nutritional and compared to control group (without oral supplements intake) for the healthy maintenance of the hair in healthy young-aged adults. Pilot studies were conducted to assess Trichovitals® on hair parameters. 1. Self-assessment questionnaire intended to perform an assessment on hair after 90 days of treatment for a group of 50 young aged volunteers. 2. Evaluated the efficacy of Trichovitals® for 90 days’ treatment by measurement of mean hair count, Pull test, Wash test, Comb and count test. In this pilot study, Volunteers expressed best results and the scores of self-assessment questionnaire were high for Overall hair volume, Scalp coverage, Thickness of hair body, Softness of hair body, Hair shine, Number of Hairs Lost onAverage Day. Oral Trichovitals® consumption was associated with significant improvement in count of terminal hairs and reduction in hair loss after 90 days of treatment. Trichovitals® achieved significant change improvement in hair than placebo group (without oral supplements intake) by 90 days of the study. From the result analysis, Trichovitals® is deemed to be one of the most effective anti-hair fall supplements. For healthy beautiful hair, enhance the appearance of hair with precision of Trichovitals® and its unique composition helps to protect hair damage, prevents hair fall and promotes hair growth. The study confirms and concludes that the Oral supplementation of 500mg/day of Trichovitals® for 12 weeks is associated with significant impact on hair thickness, reduces split ends, hair loss, hair breakage and improvement in hair growth after 90 days of treatment.Keywords
Trichovitals®, Natural Egg Shell Membrane, Collagen Type (I, III, V, X), Elastin, Oral supplement, Pilot study, Hair growth, Hair thicknessReferences
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- Yu JY et al. Preclinical and Clinical Studies Demonstrate That the Proprietary Herbal Extract DA-5512 Effectively Stimulates Hair Growth and Promotes Hair Health. Evidence-Based Complementary and Alternative Medicine. 2017; Article ID 4395638, 11 pages. https://doi.org/10.1155/2017/4395638
- Patel DP, Swink SM, Soccio LC. A Review of the Use of Biotin for Hair Loss. Skin Appendage Disord. 2017; 3 :166–169. DOI: 10.1159/000462981
- Pumthong G, Asawanonda P, Varothai S. Curcuma aeruginosa, a novel botanically derived 5a-reductase inhibitor in the treatment of male-pattern baldness: a multicenter, randomized, double-blind, placebo-controlled study. Journal of Dermatological Treatment. 2012; 23: 385–392
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