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Thangabalan, B.
- Development of Validated RP-HPLC Method for the Estimation of Itraconazole in Pure and Pharmaceutical Dosage Form
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1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
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Asian Journal of Pharmaceutical Analysis, Vol 3, No 4 (2013), Pagination: 119-123Abstract
A simple, fast and precise RP-HPLC method was developed for the quantification of Itraconazole in pure and pharmaceutical dosage form. The quantification was carried out using Dionex C18 4.6 X 250mm, 5μm enhanced polar selectivity column and mobile phase comprised of methanol and pH 7.5 potassium dihydrogen phosphate in the ratio of 40:60 and degassed under ultrasonication. The flow rate was 1.5ml/min and the effluent was monitored at 306nm. The retention time of Itraconazole was found to be 5.2 min. The method was validated in terms of linearity, precision, accuracy, specificity, robustness, limit of detection and limit of quantitation in accordance with ICH guidelines. Linearity of Itraconazole was in the range of 200-600 μg/mL. The percentage recoveries of Itraconazole were 99.33% to 99.66% from the capsule formulation. The proposed method is suitable for determination of Itraconazole in pharmaceutical dosage form.Keywords
Chromatography, Itraconazole, Method Validation.- Development and Validation of RP–HPLC Method for Simultaneous Estimation of Cefepime and Tazobactam in Injection Formulation
Abstract Views :207 |
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Authors
Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 3, No 4 (2013), Pagination: 131-137Abstract
A new, rapid, highly sensitive, economical and accurate RP-HPLC method was developed for simultaneous estimation of Cefepime and Tazobactam in injection formulation. The separation was achieved by C18 column (150 × 4.6 mm, 5 μ particle size) with mobile phase consisting of phosphate buffer (pH 2.4, diluted with orthophosphoric acid), methanol and acetonitrile in the ratio of 90:5:5 v/v, using flow rate 1.1 mL/min and eluents monitored at 260 nm. The developed method was validated as per ICH guidelines for specificity, linearity, precision, accuracy, robustness, limit of detection and limit of quantification. The retention times of cefepime and tazobactam were 2.236 and 4.498 min respectively. The linearity was found to be in the range of 250-750 μg/mL and 31.25-93.75 μg/mL for cefepime and tazobactam sodium respectively, had regression coefficients (R2) 0.999. The proposed method was successfully applied for simultaneous estimation of both drugs in injection formulation.Keywords
RP-HPLC, Cefepime, Tazobactam, Injection, Validation.- Stability Indicating RP–HPLC Method for the Estimation of Acamprosate in Pure and Tablet Dosage Form
Abstract Views :204 |
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Authors
Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 3, No 4 (2013), Pagination: 141-146Abstract
A simple, fast and precise stability indicating RP - HPLC method was developed for the separation and quantification of Acamprosate in pharmaceutical dosage form. The quantification was carried out using Hypersil C18 4.6X150 mm, 5 μm enhanced polar selectivity column and mobile phase comprised of 0.2M Ammonium acetate and acetonitrile in proportion of ratio 40:60 and degassed under ultrasonication. The flow rate was 1mL/min and the effluent was monitored at 220 nm. The retention time of Acamprosate was 4.187min. The method was validated in terms of linearity, precision, accuracy, specificity, limit of detection and limit of quantitation in accordance with ICH guidelines. Linearity of Acamprosate was in the range of 5 - 30 μg/mL. The percentage recoveries of Acamprosate was 99.00% from the tablet formulation. The stability - indicating capability was established by forced degradation experiments. The proposed method is suitable for determination of Acamprosate in pharmaceutical dosage form.Keywords
Chromatography, Acamprosate, Forced Degradation, Method Development, Method Validation.- Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Ketorolac Tromethamine and Olopatadine Hydrochloride in Pure and Pharmaceutical Formulation
Abstract Views :176 |
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Authors
Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
Source
Research Journal of Pharmaceutical Dosage Form and Technology, Vol 6, No 1 (2014), Pagination: 37-43Abstract
A simple, fast, precise, selective and accurate RP-HPLC method was developed and validated for the simultaneous determination of Ketorolac tromethamine and olopatadine HCl from bulk and formulations. Chromatographic separation was achieved isocratically on a Inertsil ODS C18 column (250×4.6 mm, 5 μ particle size) using a mobile phase 0.1 M Sodium di hydrogen orthophosphate: Acetonitrile in the ratio of 55:45. The flow rate was 1 ml/min and effluent was detected at 235nm. The retention time of Ketorolac and olopatadine were 2.507 min and 4.933 min. respectively. Linearity was observed in the concentration range of 4-24μg/ml and 12-72μg/ml for Ketorolac and olopatadine respectively with correlation coefficient of 0.999 for both the drugs. Percent recoveries obtained for ketorolac and olopatadine were 100.03% and 100.04%, respectively. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The method developed can be used for the routine analysis of Ketorolac and olopatadine from their combined dosage form.Keywords
RP-HPLC Method, UV-VIS Detection, Keterolac and Olopatadine Ophthalmic Formulation.- Stability Indicating RP-HPLC Method for the Estimation of Memantine Hydrochloride in Pure and Pharmaceutical Dosage Form
Abstract Views :171 |
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Authors
Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN