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B. G. Gaikwad ¹

Affiliations
1 C.E. Division, National Chemical Laboratory, Pune-411008, IN

Abstract

Bacterial strains and yeast strains were screened for treatment of black liquor. 17 bacterial cultures of genus - arthrobacter, cellulomonas, chainia, pseudomonas and glucanobacter were used. 19 yeast cultures having genuskluyveromyces, pachysolen, pichia, rhodotorula, saccharomyces, candida and yerrowia were used for study. At pH 8.0, 58.33% sugar utilization, 16.14% phenol degradation, 37.81% decolorization was obtained by using Candida catanula NCIM 3337, C. brumptii 3402, Pseudomonas fluorescens 2653 respectively. 45.63% sugar utilization, 29.48% phenol degradation , 20.97% decolorization was observed by using Yerrowia lipolytica NCIM 3472, Pseudomonas fluorescens 2173, Yerrowia lipolytica 3589 respectively at pH 6.5. 63.49% sugar utilization was observed by Pseudomonas fluorescens 2141 at pH 4.5. 28.85% phenol degradation was done by Pachysolen tannophilus 3445 at pH 4.5. 63.26% decolorization was observed for Candida lambica 3532 at pH 4.5. Use of biomass for decolorization was attempted in this study. Fermentation of fruits was carried out. Sapota, fig, papaya, pineapple, banana, guava and tamarind were used for fermentation. Fermentation was done by using baker's yeast. Residue from ethanol fermentation was used for decolorization of black liquor. Decolorization was obtained by residue from banana and tamarind fermentation residue was higher. It was 63.79 and 65.62% decolorization for banana and tamarind fermentation residue respectively.

Keywords

Decolorization, Candida, Sugar, Phenol, pH, Pseudomonas etc.

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B. G. Gaikwad ¹, S. H. Pisal ²

Affiliations
1 C.E. Division, National Chemical Laboratory, Pune-411008, IN
2 Y.M.College, Bharati Vidyapeeth Deemed University, Kothrud, IN

Abstract

Biodegradation of spent wash was studied. Different pH used for biodegradation of spent wash. Total 30 microbial cultures (17 bacterial and 13 yeast) used for screening of spent wash (SW) biodegradation. 16 cultures have not shown decolorization of spent wash. P. testosteronii NCIM 5098 and S. cerevisiae NCIM 3077 have shown 17.69 and 16.52 % decolorization respectively. P. testosteronii NCIM 5098 has shown maximum 17.69 % decolorization at pH 4.5. Various chemicals used for precipitation of spent wash. 1.5% Ca(OH)2 found useful for precipitation. The solution obtained after separation of precipitate used for screening. 23 cultures (12 bacterial and 11 yeast) were used for biodegradation of spent wash at pH 8. Pseudomonas syringae NCIM 5102 and Pseudomonas sp. NCIM 2223 have shown 36.72% and 36.25% decolorization respectively. From the study of screening at pH 8.0, four cultures were selected for further work at pH 6.0. Candida utilis NCIM 3055 and Pseudomonas sp. 2223 have shown 26.78 % and 27.56% decolorization at pH 6.0 respectively where the colour of spent wash decreased substantially. Candida utilis NCIM 3055 has shown sugar consumption but Pseudomonas sp. NCIM 2223 has not. Therefore, Candida utilis NCIM 3055 is the best culture for biodegradation of spent wash.

Keywords

Bacteria, Yeast, Spent Wash, Sugar, Decolorization, Fermentation Etc.

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