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Choudhary, Ashish
- Anti-Oxidant Activity of Novel 5-Substituted Arylidene-3-Substituted- Benzyl-Thiazolidine-2, 4-Diones
Authors
1 Faculty of Pharmacy, Babu Banarasi Das National Institute of Technology and Management, Lucknow 227105 (UP), IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 1 (2011), Pagination: 120-122Abstract
A series of 5-substituted-arylidene-3-substituted-benzyl-thiazolidine-2, 4-dione derivatives were synthesized from thaizolidinedione and substituted benzyl chloride followed by the addition of substituted aromatic aldehydes. All the compounds 1(a)-1(e) and 2(a)-2(c) were screened for their in vitro antioxidant activity using DPPH method. The anisaldehyde based thiazolidinedione compounds 1(a) and 2(a) displayed good activity comparable to those of standard.
Keywords
Thiazolidine-2, 4-Dione, Heterocyclic Compounds, Antioxidant Activity.- Assessment of Heavy Metal Toxicity in Four Species of Freshwater Ciliates (Spirotrichea:Ciliophora) from Delhi, India
Authors
1 Acharya Narendra Dev College, University of Delhi, Delhi 110 019, IN
2 Maitreyi College, University of Delhi, Delhi 110 021, IN
3 Department of Life Sciences, Natural History Museum, London, SW7 5BD, GB
Source
Current Science, Vol 113, No 11 (2017), Pagination: 2141-2150Abstract
In vitro laboratory experiments were conducted to determine the toxicity (per cent survival and LC50) of essential and non-essential heavy metals (cadmium, copper, nickel, lead and zinc) in four spirotrich ciliates: Euplotes sp., Notohymena sp., Pseudourostyla sp. and Tetmemena sp. isolated from three different freshwater ecosystems in the Delhi region, India. The toxicity of the heavy metals was found to vary among the different ciliates. Copper was most toxic (24 h-LC50 value ranged between 0.125 and 0.74 mg/l) and zinc was least toxic (24 h LC50 value ranged between 46.98 and 144.32 mg/l) to each of the ciliates. Of the four ciliates, Notohymena sp. had the highest tolerance limit to three heavy metals (Cu, Cd and Pb) out of the five tested. This study shows the high potentiality of using freshwater ciliates for monitoring the intensity and potency of ecological damage caused by heavy metals in aquatic ecosystems.Keywords
Ciliates, Freshwater, Heavy Metals, Toxicity.References
- Malik, D., Singh, S., Thakur, J., Singh, R. K., Kaur, A. and Nijhawan, S., Heavy metal pollution of the Yamuna River: an introspection. Int. J. Curr. Microbiol. Appl. Sci., 2014, 3, 856–863.
- Bhattacharya, A. K., Mandal, S. N. and Das, S. K., Heavy metals accumulation in water, sediment and tissues of different edible fishes in upper stretch of Gangetic West Bengal. Trends Appl. Sci. Res., 2008, 3, 61–68.
- Karbassi, A. R., Bayati, I. and Moattar, F., Origin and chemical partitioning of heavy metals in riverbed sediments. Int. J. Environ. Sci. Technol., 2006, 3, 35–42.
- Sirohi, S., Sirohi, S. P. S. and Tyagi, P. K., Impact of industrial effluents on the water quality of Kali River in different locations of Meerut, India. J. Eng. Technol. Res, 2014, 6, 43–47.
- Cui, B., Zhang, Q., Zhang, K., Liu, X. and Zhang, H., Analyzing trophic transfer of heavy metals for food webs in the newly-formed wetlands of the Yellow River Delta, China. Environ. Pollut., 2011, 159, 1297–1306.
- Ghorade, I. B., Lamture, S. V. and Patil, S. S., Assessment of heavy metal content in Godavari river water. Int. J. Res. Appl. Nat. Soc. Sci., 2014, 2, 23–26.
- Lovley, D. R., Environmental Microbe–Metal Interactions, ASM Press, Washington, DC, USA, 2000.
- Madoni, P., The acute toxicity of nickel to freshwater ciliates. Environ. Pollut., 2000, 109, 53–59.
- Madoni, P. and Romeo, M. G., Acute toxicity of heavy metals towards freshwater ciliated protists. Environ. Pollut., 2006, 141, 1–7.
- Wanick, R. C., da Paiva, T. S., de Carvalho, C. N. and da Silva-Neto, I. D., Acute toxicity of cadmium to freshwater ciliate Paramecium bursaria. Biociências (Porto Alegre), 2008, 16, 104–109.
- Twagilimana, L., Bohatier, J., Groliere, C. A., Bonnemoy, F. and Sargos, D., A new low-cost microbiotest with the protozoan Spirostomum teres: culture conditions and assessment of sensitivity of the ciliate to 14 pure chemicals. Ecotoxicol. Environ. Saf., 1998, 41, 231–244.
- Gutierrez, J. C., Amaro, F. and Martin-Gonzalez, A., From heavy metal-binder to biosensor: ciliate metallothionein discussed. Bioessays, 2009, 31, 805–816.
- Rao, A. N. and Hussain, M. M., Ecophysiological and cytopathological impact of deflin insecticide (Bacillus thuringiensis) to an unicellular ciliate protozoan, Euplotes patella. Res. J. Recent Sci., 2012, 1, 64–67.
- Turkewitz, A. P., Orias, E. and Kapler, G., Functional genomics: the coming of age for Tetrahymena thermophila. Trends Genet., 2002, 18, 35–40.
- Gilron, D. L. and Lynn, D. H., Ciliated protozoa as test organisms in toxicity assessments. In Microscale Testing in Aquatic Toxicology: Advances, Techniques and Practice (eds Wells, P. G. et al.), CRC Press, Boca Raton, FL, USA, 1998, pp. 323–336.
- Gutierrez, J. C., Martín-Gonzalez, A., Diaz, S. and Ortega, R., Ciliates as a potential source of cellular and molecular biomarkers/biosensors for heavy metal pollution. Eur. J. Protistol., 2003, 39, 461–467.
- Payne, R. J., Seven reasons why protists make useful bioindicators. Acta Protozool., 2013, 52, 105–113.
- Berger, H., Monograph of the oxytrichidae (Ciliophora, Hypotrichia). In Monographie Biologicae, Kluwer, Dordrecht, The Netherlands, 1999.
- Chapman-Andresen, C., Pinocytosis of inorganic salts by Amoeba proteus (Chaos diffluens). C. R. Trav. Lab. Carlsberg Chim., 1958, 31, 77–92.
- Dias, N. and Lima, N., A comparative study using a fluorescence-based and a direct count assay to determine cytotoxicity in Tetrahymena pyriformis. Res Microbiol., 2002, 153, 313–322.
- Diaz, S., Martin-Gonzalez, A. and Gutierrez, C. J., Evaluation of heavy metal acute toxicity and bioaccumulation in soil ciliated protozoa. Environ. Int., 2006, 32, 711–717.
- Finney, D. J., Probit Analysis, Cambridge University Press, Cambridge, 1971, 3rd edn.
- Sokal, R. R. and Rohlf, F. J., Biometry: the Principles and Practice of Statistics in Biological Research, W.H. Freeman and Company, New York, USA, 1995, 3rd edn.
- Dunnett, C. W., A multiple comparison procedure for comparing several treatments with a control. J. Am. Stat. Assoc., 1955, 50, 1096–1121.
- Nilsson, J. R., Phagotrophy in Tetrahymena. In Biochemistry and Physiology of Protozoa (eds Levandowsky, M. and Hunter, S. H.), Academic Press, New York, 1979, pp. 339–379.
- Nilsson, J. R., Effects of copper on phagocytosis in Tetrahymena. Protoplasma, 1981, 109, 359–370.
- Nilsson, J. R., How cytotoxic is zinc? A study on effects of zinc on cell proliferation, endocytosis and fine structure of the ciliate Tetrahymena. Acta Protozool., 2003, 42, 19–29.
- Chasapis, C. T., Andreini, C., Georgiopolou, A. K., Stefanidou, M. E. and Vlamis-Gardikas, A., Identification of the zinc, copper and cadmium metalloproteome of the protozoon Tetrahymena thermophila by systematic bioinformatics. Arch. Microbiol., 2017, doi:10.1007/s00203-017-1385-y.
- Dunlop, S. and Chapman, G., Detoxification of zinc and cadmium by freshwater protozoan Tetrahymena pyriformis. II. Growth experiments and ultrastructural studies on sequestration of heavy metals. Environ. Res., 1981, 24, 264–274.
- Krawczynska, W., Pivovarova, N. N. and Sobota, A., Effects of cadmium on growth, ultrastructure and content of chemical elements in Tetrahymena pyriformis and Acanthamoeba castellanii. Acta Protozool., 1989, 28, 245–252.
- Makhija, S., Gupta, R., Toteja, R., Abraham, J. S. and Sripoorna, S., Cadmium induced ultrastructural changes in the ciliate, Stylonychia mytilus (Ciliophora, Hypotrichida). J. Cell Tissue Res., 2015, 15, 5151–5157.
- Martin-Gonzalez, A., Diaz, S., Borniquel, S., Gallego, A. and Gutierrez, J. C., Cytotoxicity and bioaccumulation of heavy metals by ciliated protozoa isolated from urban wastewater treatment plants. Res. Microbiol., 2006, 157, 108–118.
- Boldrin, F., Santovito, G., Irato, P. and Piccinni, E., Metal interaction and regulation of Tetrahymena pigmentosa metallothionein genes. Protist, 2002, 153, 283–291.
- Fu, C. and Miao, W., Cloning and characterization of a new multi-stress inducible metallothionein gene in Tetrahymena pyriformis. Protist, 2006, 157, 193–203.
- Guirola, M., Perez-Rafael, S., Capdevila, M., Palacios, O. and Atrian, S., Metal dealing at the origin of the chordata phylum: the metallothionein system and metal overload response in Amphioxus. PLoS ONE, 2012, 7, e43299.
- Gutierrez, J. C., Amaro, F. and Martin-Gonzalez, A., Heavy metal whole biosensors using eukaryotic microorganisms: an updated critical review. Front. Microbiol., 2015, 6, 1–8.
- Torreggiani, A., Chatgilialoglu, C., Ferreri, C., Melchiorre, M., Atrian, S. and Capdevila, M., Non-enzymatic modifications in metallothioneins connected to lipid membrane damages: structural and biomimetic studies under reductive radical stress. J. Proteomics, 2013, 92, 204–215.
- Klaassen, C. D., Liu, J. and Choudhuri, S., Metallothionein: an intracellular protein to protect against cadmium toxicity. Annu. Rev. Pharmacol. Toxicol., 1999, 39, 267–294.
- Brady, D., Letebele, B., Duncan, J. R. and Rose, P. D., Bioaccumulation of metals by Scenedesmus, Selenastrum and Chlorella algae. Water SA, 1994, 20, 213–218.
- Vymazal, J., Toxicity and accumulation of cadmium with respect to algae and cyanobacteria: a review. Environ. Toxicol., 1987, 2, 387–415.
- Madoni, P., Davoli, D. and Gorbi, G., Acute toxicity of lead, chromium, and other heavy metals to ciliates from activated sludge plants. Bull. Environ. Contam. Toxicol., 1994, 53, 420–425.
- Martin-Gonzalez, A., Benítez, L., Soto, T., Rodriguez de Lecea, J. and Gutierrez, J. C., A rapid bioassay to detect mycotoxins using a melanin precursor overproducer mutant of the ciliate Tetrahymena thermophila. Cell Biol. Int., 1997, 21, 213–221.
- Nicolau, A., Martins, M. J., Mota, M. and Lima, N., Effect of copper in the protistan community of activated sludge. Chemosphere, 2005, 58, 605–614.
- Amin, N. M., Techniques for assessment of heavy metal toxicity using Acanthamoeba sp., a small, naked and free-living amoeba. In The Functioning of Ecosystems (eds Ali, M.), InTech, 2012, pp. 199–212.
- Madoni, P., Esteban, G. and Gorbi, G., Acute toxicity of cadmium, copper, mercury, and zinc to ciliates from activated sludge plants. Bull. Environ. Contam. Toxicol., 1992, 49, 900–905.
- Rico, D., Martin-Gonzalez, A., Diaz, S., de Lucas, P. and Gutierrez, J. C., Heavy metals generate reactive oxygen species in terrestrial and aquatic ciliated protozoa. Comp. Biochem. Physiol – Part C, 2009, 149, 90–96.
- Nicolau, A., Mota, M. and Lima, N., Physiological responses of Tetrahymena pyriformis to copper, zinc, cycloheximide and Triton X-100. FEMS Microbiol. Ecol., 1999, 30, 209–216.
- Chaudhry, R. and Shakoori, A. R., Characterization of copper resistant ciliates: potential candidates for consortia of organisms used in bioremediation of wastewater. Afr. J. Biotechnol., 2011, 10, 9101–9113.
- Gallego, A., Martín-González, A., Ortega, R. and Gutiérrez, J. C., Flow cytometry assessment of cytotoxicity and reactive oxygen species generation by single and binary mixtures of cadmium, zinc and copper on populations of the ciliated protozoan Tetrahymena thermophila. Chemosphere, 2007, 68, 647–661.
- Parker, J. G., Toxic effects of heavy metals upon cultures of Uronema marinum (Ciliophora: Uronematidae). Mar. Biol., 1979, 54, 17–24.
- Kim, S. H., Jung, M. Y. and Lee, Y. M., Effect of heavy metals on the antioxidant enzymes in the marine ciliate Euplotes crassus. Toxicol. Environ. Health Sci., 2011, 3, 213–219.
- Coppellotti, O., Sensitivity to copper in a ciliate as a possible component of biological monitoring in the Lagoon of Venice. Arch. Environ. Contam. Toxicol., 1998, 35, 417–425.
- Self-Perceived Competence of Contemporary Restorative Practices of Dental Students in North India
Authors
1 Department of Conservative Dentistry and Endodontics, IGGDC JAMMU, Jammu, Jammu & Kashmir – 180005, IN
2 Registrar, Department of Conservative Dentistry and Endodontics, IGGDC JAMMU, Jammu, Jammu & Kashmir – 180005, IN
3 Private Practitioner, General Dentistry, New Delhi –100008, Delhi, IN
4 Department of Periodontology, Teerthanker Mahaveer Dental College and Research Centre, Moradabad–244003, Uttar Pradesh, IN
Source
International Journal of Medical and Dental Sciences, Vol 10, No 1 (2021), Pagination: 1938-1944Abstract
Background: There has been a paradigm shift in the restorative dentistry practiced in the clinics with an increased share of resin-composites in contrast to dental amalgam due to various factors, however, this has not found its way into the Indian dental school curriculum. Aim: To evaluate the self-perceived competence of contemporary restorative practices of dental students in North India. Materials and Methods: 232 dental students from 4 colleges in North India completed a survey comprising 11 polar questions about their perception of contemporary restorative dental practice and their curriculum update regarding the same. Further, the collected data was tabulated and finally extracted data was subjected to descriptive analysis using Chi-square test. Results: All 11 questions showed statistically significant responses. Only 15.5% of students thought that the current curriculum of restorative dentistry was adequate. Moreover, only 20.7% of the respondents were confident about using rubber dam for isolation. About 93.1% felt that the quality of their composite restorations was not at par with those seen on social media. A clear majority of 91.4% felt that the maximum uploads of restorative dentistry involved composite restorations. A huge number of 91.45% did not use rubber dam for isolation in composite restorations. 79.3% students felt that the current curriculum is not teaching them about the various instruments, and developing their skills about instrumentation and operative procedures of composite restorations matching the trending composite practices. Conclusion: Considering the results of this study as the student’s mandate will help shape the guidelines for possibly a new curriculum of conservative dentistry in India.
Keywords
Conservative dentistry, restorations, dental education, teachingConservative dentistry, restorations, dental education, teaching, resin-base composites, dental amalgam.References
- Lagali-Jirge V, Umarani M. Evaluation of readiness to practice among interns at an Indian dental school. J Contemp Med Edu. 2014; 2(4): 227–231. https://doi.org/10.5455/jcme.20141105071940
- Kaul V, Kaul R, Ahmed R, Singh S, Khateeb SU. Stress, stressors and psychological disturbances in undergraduate students at a dental college in Jammu and Kashmir: A crosssectional study. Int Dent J Stu Res. 2017; 5(4): 100–108.
- McComb D. Class I and Class II Silver Amalgam and Resin Composite Posterior Restorations: Teaching Approaches in Canadian Faculties of Dentistry. J Can Dent Assoc 2005; 71(6): 405–6.
- Sadeghi M, Lynch CD, Wilson NH. Trends in dental education in the Persian Gulf--an example from Iran: contemporary placement of posterior composites. Eur J Prosthodont Restor Dent. Dec 2009; 17(4): 182–7.
- Roeters J J M, Shortall A C C, Opdam N J M. Can a single composite resin serve all purposes? Br Dent J 2005; 199: 73–79. https://doi.org/10.1038/sj.bdj.4812520
- Burke F J T, McHugh S, Hall A C et al. Amalgam and composite use in UK general dental practice in 2001. Br Dent J 2003; 194: 613–618. https://doi.org/10.1038/sj.bdj.4810258
- Brown L J, Wall T, Wassenaar J D. Trends in resin and amalgam usage as recorded on insurance claims submitted by dentists from the early 1990s and 1998. J Dent Res 2000; 79: 461.
- Jirau-Colón H, González-Parrilla L, Martinez-Jiménez J, Adam W, Jiménez-Velez B. Rethinking the Dental Amalgam Dilemma: An Integrated Toxicological Approach. Int J Environ Res Public Health. 22 Mar 2019; 16(6). pii: E1036. https://doi.org/10.3390/ijerph16061036
- Lynch CD, McConnell RJ, Wilson NH. Challenges to teaching posterior composites in the United Kingdom and Ireland. Br Dent J. 23 Dec 2006; 201(12): 747–50. https://doi.org/10.1038/sj.bdj.4814348
- Mala S, Lynch CD, Burke FM, Dummer PM. Attitudes of final year dental students to the use of rubber dam. Int Endod J. Jul 2009; 42(7): 632–8. https://doi.org/10.1111/j.1365-2591.2009.01569.x
- Roeters F J, Opdam N J, Loomans B A. The amalgamfree dental school. J Dent 2004; 32: 371–377. https://doi.org/10.1016/j.jdent.2004.02.008
- AL-Rabab’ah MA, Bustani MA, Khraisat AS, Sawair FA. Phase down of amalgam: awareness of Minamata convention among Jordanian dentists. Saudi Med J. Dec 2016; 37(12): 1381. https://doi.org/10.15537/smj.2016.12.16163
- G S, Jena A, Maity AB, Panda PK. Prevalence of Rubber Dam Usage During Endodontic Procedure: A Questionnaire Survey [Internet]. June 2014 [Cited January 7, 2021]; 8(6): ZC01–ZC03