Informatics Publishing Limited

Justina Daphne ¹, Asha Francis ¹, Ria Mohanty ¹, Nupur Ojha ¹, Nilanjana Das ¹

Affiliations
1 Bioremediation Laboratory, Department of Bio-Medical Sciences, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN

Abstract

The present study had investigated the role of yeasts, isolated from soil and food samples for its ability to synthesize silver nanoparticles. Serial dilution method followed by spread plate technique was used for isolation of yeasts on YEPD agar medium and the morphological characterization was done by simple staining technique. Primary screening was done by visual colour change followed by UV spectrophotometric analysis. Significant color change and prominent peaks around 420-450 nm were noted through UV spectrophotometric analysis in the cell free extract of Isolate 5, Isolate 8 and Isolate 13, revealed the synthesis of Ag-NPs. Optimization of growth parameters for the enhancement of Ag-NPs production was evaluated for each of the potent yeast isolates. Isolate 5 and 13 had showed highest Ag-NPs production after 24 h of incubation period, supplemented by fructose in the production medium of pH 9 along with 5mM substrate concentration of AgNO₃, at 45˚C, whereas in case of isolate 8, highest production was observed with 3mM concentration of Ag NO₃ at pH 8. FTIR analysis revealed the presence of significant peaks around 3441-3255, 2922-2964, 1576-1579, 1384-1415, 1276, 1153 and 1076cm^-1 of Ag-NPs synthesized by isolate 5,8 and 13 respectively, were corresponding to the functional groups produced during the synthesis of Ag-NPs. XRD results showed peaks at 2 theta values 27.29°, 31.76°, 45.91°, 54.26-56.77° and 75.56-75.99°, were corresponding to (111), (200), (222), (311), and (331) lattice of silver nanoparticles. Distinct surface topology of the AgNPs synthesised by the potent yeast isolates were observed by AFM analysis. Positive anti-oxidant and anti-bacterial activity were elucidated by AgNPs synthesised by the potent yeast isolates, wherein AgNPs were found to be bacteriostatic at low concentration (5ug/ml) and bactericidal at high concentrations (100ug/ml).

Keywords

Yeast Strains, Silver Nanoparticles, Optimization, FTIR, XRD, AFM, Antibacterial Activity.

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Feona George ¹, Greeshma Raju ¹, Jency Xavier ¹, Jenisha J. B. ¹, Shilpa S. ¹, Asha Francis ²

Affiliations
1 Third Year BSc Nursing Students, Bishop Benziger College of Nursing, Kollam, IN
2 Nursing Tutor, Department of Child Health Nursing, Bishop Benziger College of Nursing, Kollam, IN

Abstract

A study to assess the effectiveness of Self-Instructional Module on knowledge regarding Methicillin Resistant Staphylococcus aureus (MRSA) infection among staff nurses of a selected hospital, Kollam. The objectives of the study, a) To assess the knowledge of staff nurses regarding Methicillin Resistant Staphylococcus aureus (MRSA) infection. b) To find the association between knowledge of staff nurses regarding MRSA infection and selected demographic variables such as education, working unit, years of experience and membership in infection control team. c)To determine the effectiveness of Self-Instructional Module on knowledge regarding Methicillin Resistant Staphylococcus aureus infection among staff nurses. A quantitative approach was used with one group pretest posttest only design. Convenience sampling was used with a sample size of 50 staff nurses. The investigator assessed staff nurses knowledge using structured questionnaire regarding Methicillin Resistant Staphylococcus aureus (MRSA) infection. After conducting the pretest, self instructional module was introduced to the staff nurses. Posttest was conducted on fifth day using the same research tool. It is found that the calculated t value is greater than table value. There is significant difference between pretest and posttest scores of knowledge of staff nurse. There is no significant association between knowledge and year of experience, membership in infection control team. Whereas educational qualification and working unit had significant association between knowledge regarding Methicillin Resistant Staphylococcus aureus (MRSA) infection. The finding of the study suggests that the self instructional module was effective in increasing the knowledge of staff nurses regarding Methicillin Resistant Staphylococcus aureus (MRSA) infection.

Keywords

Assess, Effectiveness, Self Instructional Module, Knowledge, Methicillin Resistant Staphylococcus aureus (Mrsa) Infection, Staff Nurse.

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