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- P. Deepesh
- G. Shanmuga Priya
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- Aileen Rose Edwin
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- A. Rubiga
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Das, Arunava
- Evaluation of Engineering Properties of Problematic Soils using Bacillus pasteurii
Abstract Views :165 |
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Authors
Affiliations
1 Department of Civil Engineering, Kongu Engineering College, Perundurai, Erode (dist.), T. N– 638060, IN
2 Department of Civil Engineering, Bannari Amman Institute of Technology, Sathyamangalam, Erode (Dist.), T.N– 638401, IN
3 Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode (Dist.), T.N-638401, IN
4 Department of Civil Engineering, Kongu Engineering College, Perundurai, Erode (Dist.), T.N– 638 060, IN
1 Department of Civil Engineering, Kongu Engineering College, Perundurai, Erode (dist.), T. N– 638060, IN
2 Department of Civil Engineering, Bannari Amman Institute of Technology, Sathyamangalam, Erode (Dist.), T.N– 638401, IN
3 Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode (Dist.), T.N-638401, IN
4 Department of Civil Engineering, Kongu Engineering College, Perundurai, Erode (Dist.), T.N– 638 060, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 5 (2019), Pagination: 2098-2102Abstract
Soil improvement method using enzymatic activity from bacteria Bacillus pasteurii that able to catalyse urea and calcium chloride enabling cementation solution has become an emerging approach to increase the shear strength of sand. The production of urease enzyme of B. pasteurii will be increased using specific medium. Determination of the volume of bacteria needed is based on the soil porosity is studied and analysed. The preliminary result of B. pasteurii that use specific medium could increase the urease enzyme activity and already verified using urease measurement kit. The treatment period required for the initiation of production of cementing material (calcite) has been reduced from one month to 3 days, while using this specific medium for enzyme urease production. This leads to the rapid filling of the soil pores that result in the hardening of soil. The bonding between the soil particles also increases that leads to increase in the density of soil. The final results of shear improvement are obtained by conducting Direct shear test and Unconfined compression test on sandy and silty soils respectively. Finally the cohesion between the soil particles was identified through the permeability test.Keywords
B. pasteurii, Direct Shear Test, Permeability, Soil Pores, Unconfined Compression Test.References
- Apichit Kampala and Suksun Horpibulsuk. Engineering Properties of Silty Clay Stabilized with Calcium Carbide Residue. Asce journal of materials in civil engineering. May 2013; 25(5): 632-644.
- Achal V., Pan X. Characterization of Urease and Carbonic Anhydrase Producing Bacteria and Their Role in Calcite Precipitation. Springer Science + Business Media. LLC.2010.
- Pham et.al,. Evaluating Strategies to Improve Process Efficiency of Denitrification -Based micp. Asce journal of geotechnical & geoenvironmental engineering. 2018; 144(8): 04018049. ISSN 1090-0241.
- Soon et.al,. Factors Affecting Improvement in Engineering Properties of Residual Soil through Microbial-Induced Calcite Precipitation. Asce Journal Of Geotechnical & Geoenvironmental Engineering. March- 2014; 140(5): 04014006.
- Sun et.al,. Improvement of Microbial-Induced Calcium Carbonate Precipitation Technology for Sand Solidification. ASCE Journal Of Materials In Civil Engineering. Aug-2018; 30(11): 04018301.
- Wang et.al,. Surficial Soil Stabilization against Water-Induced Erosion Using Polymer-Modified Microbially Induced Carbonate Precipitation. Asce Journal Of Materials In Civil Engineering. 2018; 30(10): 04018267.
- Ivanov V., Chu J. Applications of microorganisms to geotechnical engineering for bio-clogging and bio-cementation of soil in situ. Springer Science+Business Media B.V. 2008.
- Guzmán M, Jean GD, Stephan G; Synthesis of silver nano-particles by chemical reduction method and their antibacterial activity. International Journal of Chemical and Biomolecular Engineering 2009; 2: pg-104-111.
- S. Karthick Raja Namasivayam1, S. Nivash Kumar, Chandra Mohan. S, V. Gayathri. Biocompatible formulation of Probiotic bacteriaBacillus subtilis with agriculture based Products for the effective acid Tolerance Properties and Synergistic activity with Antibiotics. Research J. Pharm. and Tech. 2018; 11(1): 73-78
- Salwa M. Al-Thawadi. High strength Instiu biocementation of soil by calcite precipitating locally isolated ureolytic bacteria.Ph.d. thesis book. 2008.
- Soon et.al,. Improvements in engineering properties of soils through microbial-induced calcite precipitation. KSCE Journal of Civil Engineering. July- 2013; 17(4):718-728.
- Thullner M., et al. Modeling of a microbial growth experiment with bioclogging in a two-dimensional saturated porous media flow field. 2003.
- Tittelboom et al. Use of bacteria to repair cracks in concrete. Elsevier. 2009.
- Angayarkanni T., Anitha Subash, Tamilselvi V., Kamalakannan A. Antimicrobial Compounds Production by Pseudomonas fluorescens and Bacillus subtilis. Asian J. Research Chem. January 2012;5(1): 61-64
- Prerana H. Salodkar, Nikhil A. Maske, Dipali H. Chaudhari. Effect of Nylon Fiber, Fly Ash and Lime utilization for different Soil stabilization conditions: A Case Study. Research J. Science and Tech. Oct. – Dec. 2015;7(4):223-229.
- Advait N. Bhange, Nikhil A. Maske, P. Salodkar. Utilization of Fly Ash, Lime and Synthetic Bag Fiber for Soil Stabilization. Research J. Engineering and Tech. 2014; 5(4): 195-203.
- Govindasamy Vinodhini, et.al,. Assessment of Antimicrobial Property of a Secondary Metabolite Produced by an Enriched Bacterial Culture Isolated from Soil. Research J. Pharm. and Tech. Jan. 2015; 8(1): 51-53.
- vanPaassen L.A. et al. Potential Soil Reinforcement by Biological Denitrification. http://dx.doi.org/10.15242/IICBE.C514577 25.2009.
- Arya C F, et.al,. Microbial geotechnology: Evaluation of strength and structural properties of microbial stabilized mud block (MSMB). International Journal of Scientific & Engg Research. Jan.2016; Vol-7, issue-1. ISSN 2229-5518.
- Deblina D, et.al,. Evaluation of Biosurfactant producing activity of Bacillus thuringiensis-VITNDUVNB04 isolated from oil contaminated soil by various methods. Research J. Pharm. and Tech 2016; 9(12):2186-2190.
- K. Natarajan, et.al,.. A Study on Tri Calcium Phosphate Solubilisation Efficiency of Soil Microbes. Asian J. Research Chem. 3(4): Oct. - Dec. 2010; Page 1054-1057.
- Abhijit Ray. Isolation and screening of antibiotic producing Actinomyces species from soil sample. Research J. Engineering and Tech. 3(3): July-Sept. 2012 page 199-201.
- In Vitro Antibacterial and Anticancer Study of Bioactive Compounds Isolated from Punica Grantum Peel
Abstract Views :364 |
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Authors
Affiliations
1 Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
1 Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
Source
Indian Journal of Public Health Research & Development, Vol 11, No 1 (2020), Pagination: 45-52Abstract
Plants have been used as natural medicines since the dawn of human kind. This research was focused on phytochemical analysis of Punica grantum peel extract consist of ethanol: chloroform (99:1) as solvent and its constituents were molecularly characterized by GCMS analysis and its ability as an antioxidant, anticancer against K562 cell and antibacterial against some food borne pathogens which comprises of 5 gram-positive and 3-gram-negative bacteria. The phytochemical analysis was performed biochemically and results reveals the presence of secondary metabolites such as Flavonoids, Alkaloids, Tannins, Saponins, etc. and absence of glycosides. The extract was tested against both gram-positive and gram-negative bacteria by agar well diffusion method and its results have shown that the streptococcus faecalis have shown highest susceptibility against the extract. Its antioxidant potential was quantitatively determined by DPPH assay and results showed high range of antioxidant present in the extract with IC50 as 504.9μg/ml. The cytotoxicity assay was performed with peel extract of different concentration against K562 cancer lines which is a myelogenous leukemia cell line. However, the cytotoxicity is less when compared with cell viability and its effects were mild against the cancer cells.Keywords
Punicagranatum, DPPH, GCMS, K562 Cancer Cells (Myelogenous Leukemia).- In Vitro Anticancer Study of Bioactive Compound Isolated from Musa Extract (Musa acuminata)
Abstract Views :397 |
PDF Views:0
Authors
Affiliations
1 Associate Professor, Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
2 Research Associate cum Assistant Professor, Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
3 III Year Biotech Students, Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
1 Associate Professor, Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
2 Research Associate cum Assistant Professor, Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
3 III Year Biotech Students, Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
Source
Indian Journal of Public Health Research & Development, Vol 11, No 1 (2020), Pagination: 340-346Abstract
Banana tree being one of the most economically important tree worldwide is cut once the fruits are harvested. However other parts of the tree are used in medicine worldwide. In this study, the flower extract of Musa acuminatais extracted by maceration with methanol and the phenolic and aliphatic compounds present in the flower extract is identified using GC-MS analysis. Phytochemical screening of the banana extract showed the presence of alkaloids, phlobatanning, triterpanoids, flavanoids, lipids, steroids and terpenoids. Potent antibacterial activity is observed from the flower extract against the tested gram positive and negative bacteria. Anticancer activity of the flower extract is assessed on the cervical cancer cell line HeLa. MTT assay is used to evaluate the antiproliferative effects. This study concluded that the economically important Musa acuminatais widely used in anticancer studies worldwide.Keywords
Musa acuminata, Phenolic Compounds, GC-MS Analysis, Phytochemical Screening, Antibacterial Activity, MTT Assay.- Comparative Analysis of Bioactive Compounds and Anticancerous Activities in Leaf and Stem Extract of Physalis minima
Abstract Views :251 |
PDF Views:198
Authors
Affiliations
1 Molecular Diagnostics and Biomolecular Characterization Laboratory, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode District, Tamil Nadu, IN
1 Molecular Diagnostics and Biomolecular Characterization Laboratory, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode District, Tamil Nadu, IN
Source
Journal of Natural Remedies, Vol 20, No 1 (2020), Pagination: 42-52Abstract
Physalis minima, commonly known as native gooseberry is a perennial herb which grows mostly in the tropical regions and is used in the traditional medicines as a curative for gastric ulcers and also for respiratory tract related ailments. The leaf and stem crude extract was separated by soxhelet using chloroform as solvent followed by the phytochemical screening to identify the presence of secondary metabolites. The Fourier-transform infrared spectroscopy (FTIR) showed the presence of various functional group such as phenol, alkane, aldehyde, alkyls, amino acid, aromatic amino acids and secondary alcohol. The Gas chromatography-mass spectrometry (GC-MS) result showed the presence of total of 24 bioactive compounds in both extracts such as Heptacosane, 1-chloro, Heptacosane, Octadecanoic acid, N-hexadecanoic acid and Hexatriacontane. The extract was subjected to for its anti-cancerous activity using MTT assay which had a good result against HL60 cell line and antibacterial activities.Keywords
HL60, MTT Assay, Physalis minima, Phytochemical Screening, Soxhlet Extraction.References
- Mohana K, Purushothaman KK. Antifertility properties of Physalis minima. Bulletin of Medico-Ethno-Botanical Research. 1981; 2:135–43.
- Singh S, Ehana NM, Dhar MM. Solaplumbin: An anticancer glycoside from Nicotiana plumbaginifolia. Phytochemistry, 1974; 13:2020–2.
- Okwu DE, Josaiah C. Evaluation of the chemical composition of two Nigerian medicinal plants. African Journal of Biotechnology. 2006; 5:357–61.
- Dahanukar SA, Kulkarni RA, Rege NN. Pharmacology of medicinal plants and natural products. Indian Journal of Pharmacology. 2000; 32:S81–118.
- Nathiya M, Dorcus D. Preliminary phytochemical and antibacterial studies on Physalis minima Linn. International Journal of Current Science. 2012:24–30.
- Parmar C, Kaushal MK. Physalis minima. In: Wild Fruits. New Delhi, India: Kalyani Publishers; 1982.
- Tiger L. The natural guide to the medicinal herbs and plants (1st ed.), Tigerbooks, PLS, Twitchenhanze, UK; 1980. p. 12–15.
- Karpagasundari C, Kulothungan S. Analysis of bioactive compounds in Physalis minima leaves using GC MS, HPLC, UV-VIS and FTIR techniques. Journal of Pharmacognosy and Phytochemistry. 2014; 3(4):196–201.
- Vinson JA, Dabbagh YA, Serry MM, Jang J. Plant flavonoids, especially tea flavonoids are powerful antioxidant using an invitro antioxidant model for heart disease. Journal of Agricultural and Food Chemistry. 1995; 43:2800–2.
- Agbor AG, Talla L, Ngogang JY. The Antidiarrhoeal activity of Alchornea cordifolia leaf extract. Phytotherapy Research: PTR. 2004; 18(11):873–6.
- Chothani DL, Vaghasiya HU. A phyto-pharmacological overview on Physalis minima Linn. Indian Journal of Natural Products and Resources. 2012; 3:477–82.
- Ramkumar KM, Rajaguru IP, Ananthan ZR. Antimicrobial properties and phytochemical constituents of an antidiabetic plant Gymnema montanum. IDOSI publications. Advances in Biological Research. 2007; 1(1–2):67–71.
- Aqil F, Ahmed I, Mehmood Z. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turkish Journal of Biology. 2006; 30:177–83.
- Sumathy V, Lachumy SJ, Zakaria Z, Sasidharan S. In vitro bioactivity and phytochemical screening of Musa acuminata flower. Pharmacologyonline. 2011; 2:118–27.
- Antibacterial and in vitro Anticancer Study of Methanol Extracts of Clitoria ternatea Leaves
Abstract Views :310 |
PDF Views:401
Authors
Arunava Das
1,
G. Shanmuga Priya
1,
S. Soundariya
1,
P. Deepesh
1,
Aileen Rose Edwin
1,
E. Vihashinee
1,
A. Rubiga
1,
S. Megavarthini
1,
R. Eswaran
1,
J. Bindhu
1
Affiliations
1 Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode, IN
1 Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode, IN
Source
Journal of Natural Remedies, Vol 20, No 2 (2020), Pagination: 96-102Abstract
Clitoria ternatea is often known as butterfly pea plant by the natives and is one of the major plants used in Ayurveda and traditional medicine worldwide. It is also used in food and cosmetic industries for various purposes. In this study, methanol leaf extract of Clitoria ternatea was studied for its antibacterial and in vitro anticancer study against human promyelocytic leukemia cells (HL60). Secondary metabolites like alkaloids, phlobatannin, triterpenoids, flavonoids, lipids, steroids, terpenoids, tannins and glycosides are identified in the extract by phytochemical screening. Leaf extract showed the presence of ethers and carboxylic acid groups (GCMS analysis). The leaf extract showed resistance against Salmonella typhi. Thus, from this study it can be concluded Clitoria ternatea leaves methanol extract has bioactive compounds showing anticancer activity against HL60 cells.Keywords
Antibacterial Activity, Anticancer Activity, Clitoria ternatea, GC-MS Analysis, Phytochemical Screening, MTT Assay.References
- Balaji KS, Priyanka S, Preethi SD, Chandrashekara KT, Lokesh S. Angio-suppressive effect of Clitoria ternatea flower extracts is mediated by HIF-1α and down-regulation of VEGF in Murine carcinoma model. Medicinal Chemistry (Los Angeles). 2016; 6:515–20. https://doi.org/10.4172/2161-0444.1000392
- Chakraborthy GS, Kumar V, Gupta S, Kumar A, Gautam N, Kumari L. Phytochemical and pharmacological aspects of Clitoria ternatea-a review. Journal of Applied Pharmaceutical Sciences and Research. 2018:3–9. https://doi.org/10.31069/apsr.v1i2.13061
- Deepika S, Selvaraj I, Anbalagan M. Phytochemical characterization and cancer cell line cytotoxicity of Clitoria ternatea. Bangladesh Journal of Pharmacology. 2018; 13(4):349–52. https://doi.org/10.3329/bjp.v13i4.38612
- Divya A, Anbumalarmathi J, Sharmili SA. Phytochemical analysis, antimicrobial and antioxidant activity of Clitoria ternatea blue and white flowered leaves. Advances in Research. 2018:1–13. https://doi.org/10.9734/AIR/2018/39030
- Lakshmi NDM, Mahitha B, Madhavi T, Sushma NJ. Phytochemical screening and ftir analysis of Clitoria ternatea leaves. International Journal of Scientific and Engineering Research. 2015; 6(2):287–90.
- Jacob L, Latha MS. Anticancer activity of Clitoria ternatea Linn. against Dalton’s lymphoma. International Journal of Pharmacognosy and Phytochemical Research. 2012; 4(4):207–12.
- Jamil N, Zairi MNM, Nasim NAIM, Pa’ee F. Influences of environmental conditions to phytoconstituents in Clitoria ternatea (Butterfly Pea Flower)-A Review. Journal of Science and Technology. 2018; 10(2).
- Kamilla L, Mansor SM, Ramanathan S, Sasidharan S. Effects of Clitoria ternatea leaf extract on growth and morphogenesis of Aspergillus niger. Microscopy and Microanalysis. 2009; 15(4):366–72. https://doi.org/10.1017/S1431927609090783. PMid:19575837
- Kumar D, Dhobi M. Antianxiety and antioxidant profile of blue and white variety of Clitoria ternatea L. Indian Journal of Research in Pharmacy and Biotechnology. 2016; 4(3):90.
- Lijon MB, Meghla NS, Jahedi E, Rahman MA, Hossain I. Phytochemistry and pharmacological activities of Clitoria ternatea. International Journal of Natural and Social Sciences. 2017; 4(1):1–10.
- Mathew N, Anitha MG, Bala TSL, Sivakumar SM, Narmadha R, Kalyanasundaram M. Larvicidal activity of Saracaindica, Nyctanthesarbor-tristis, and Clitoria ternatea extracts against three mosquito vector species. Parasitology Research. 2009; 104(5):1017–25. https://doi.org/10.1007/s00436-008-1284-x. PMid:19039604
- Nguyen KNT, Nguyen GKT, Nguyen PQT, Ang KH, Dedon PC, Tam JP. Immunostimulating and Gram‐negative‐specific antibacterial cyclotides from the butterfly pea (Clitoria ternatea). The FEBS Journal. 2016; 283(11):2067–90. https://doi.org/10.1111/febs.13720. PMid:27007913
- Pasukamonset P, Kwon O, Adisakwattana S. Alginatebased encapsulation of polyphenols from Clitoria ternatea petal flower extract enhances stability and biological activity under simulated gastrointestinal conditions. Food Hydrocolloids. 2016; 61:772–9. https://doi.org/10.1016/j.foodhyd.2016.06.039
- Kumar TR, Kumar S, Anju VS. Phytochemical and antibacterial activities of crude leaf and ischolar_main extracts of Clitoria ternatea varieties (Fabaceae). Journal of Pharmacognosy and Phytochemistry. 2017; 6(6):1104–8.
- Sarumathy K, Rajan MD, Vijay T, Jayakanthi J. Evaluation of phytoconstituents, nephro-protective and antioxidant activities of Clitoria ternatea. Journal of Applied Pharmaceutical Science. 2011; 1(5):164–72.
- Antimicrobial, Antioxidant and Anticancer Activity of the Ethanol Extract of Pleurotus ostreatus
Abstract Views :281 |
PDF Views:243
Authors
J. Bindhu
1,
Arunava Das
1,
E. Vihashinee
1,
A. Rubiga
1,
Aswin Edward
1,
R. Eswaran
1,
G. Shanmuga Priya
1,
S. Soundariya
1,
Aileen Rose Edwin
1
Affiliations
1 Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode District, Tamil Nadu, IN
1 Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode District, Tamil Nadu, IN
Source
Journal of Natural Remedies, Vol 20, No 2 (2020), Pagination: 110-115Abstract
The current study evaluates antimicrobial, antioxidant and anticancer activity of the ethanol extract of Pleurotus ostreatus. Chemical analysis showed the presence of alcohol, amides, alkanes, ether, phenol and sulphides. Most of the chemical constituents were found to be esters and carboxylic acid derivatives. The extract showed highest resistant against Bacillus cereus. P. ostreatus ethanol extract showed comparable antioxidant activity with ascorbic acid in both DPPH and FRAP method and also showed an IC50 of 39.29 μg/ml against HL60 cells.Keywords
MTT, DPPH, HL-60 Cells, FRAP, GC-MS.References
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- Singh V, Pandey R, Vyas D. Antioxidant potentiality of Pleurotus ostreatus (MTCC142) cultivated on different agro wastes. Asian Journal of Plant Science and Research. 2015; 5:22-7.
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- Authenticating the Anti-cancer Properties of Couroupita guianensis in Western Ghats using HL60 Humanleukemia Cell Line
Abstract Views :247 |
PDF Views:122
Authors
J. Bindhu
1,
R. Felicia Roshini
1,
M. Monica Devi
1,
Arunava Das
1,
R. Balakrishna Raja
1,
S. Tamilselvi
1
Affiliations
1 Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Tamil Nadu, IN
1 Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Tamil Nadu, IN