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Kshirsagar, Ajay
- Hepatoprotective and Antioxidant Potential of Calotropis gigantea in Cyclosporine-An Induced Hepatotoxicity
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Authors
Ajay Kshirsagar
1,
Deepa Ingawale
2,
Purnima Ashok
3,
Vrushali Thorve
2,
Tanmay Dodal
2,
Anurag Dodal
2,
Mahesh Kahane
2,
Bharat Zope
2
Affiliations
1 Pad. Dr. D. Y. Patil Institute of Pharmaceutical sciences and research, Pimpari, Pune-411 018, India, IN
2 AISSMS College of Pharmacy, Near RTO, Kennedy road, Pune-411 001, IN
3 Department of Pharmacology, K.L.E.S's College of Pharmacy, Bangalore-560010, IN
1 Pad. Dr. D. Y. Patil Institute of Pharmaceutical sciences and research, Pimpari, Pune-411 018, India, IN
2 AISSMS College of Pharmacy, Near RTO, Kennedy road, Pune-411 001, IN
3 Department of Pharmacology, K.L.E.S's College of Pharmacy, Bangalore-560010, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 2, No 5 (2010), Pagination: 343-347Abstract
The ethanolic fraction of Calotropis gigantea flowers (CGFE) was evaluated for its possible hepatoprotective potential in Wistar rats. The CGFE (250mg/kg and 500mg/kg, bw p.o.) showed a remarkable hepatoprotective activity against cyclosporine-A induced hepatotoxicity as judged from the level of serum markers for liver damage. Cyclosporine-A induced a significant rise in serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP) and lipid profile levels. The cotreatment of animals with CGFE (250mg/kg and 500mg/kg, p.o.) significantly decreased the elevated serum marker enzyme and lipid profile levels near to normal. The activity of the CGFE was comparable to the standard drug, silymarin (100mg/kg, p.o.). Further histopathological studies support the above finding.Keywords
Antioxidant, Calotropis gigantea, Cyclosporine-A, Hepatotoxicity.References
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- Stability Indicating Hplc Method Development and Validation For Simultaneous Estimation of Metformin and Empagliflozin in Bulk And Pharmaceutical Dosage Form
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Authors
Affiliations
1 School of Pharmacy, Swami Ramanand Teerth Marathwada University, Vishnupuri, Nanded 431606, (M.S.), IN
2 D. K. Patil Institute of Pharmacy, Loha 431708, Dist-Nanded, (M.S.), IN
1 School of Pharmacy, Swami Ramanand Teerth Marathwada University, Vishnupuri, Nanded 431606, (M.S.), IN
2 D. K. Patil Institute of Pharmacy, Loha 431708, Dist-Nanded, (M.S.), IN
Source
Research Journal of Pharmacy and Technology, Vol 15, No 2 (2022), Pagination: 830-836Abstract
In the present work, simple, accurate and rapid stability indicating RP-HPLC method has been developed for the simultaneous estimation of Metformin and Empagliflozin in bulk and tablet dosage form. Separation of analytes was carried out on Ascentis C18 HPLC column (4.6 x 150 mm, 5 μm) using a mobile phase of 0.1% orthophosphoric acid and acetonitrile (60:40, v/v), at a flow rate of 1.0mL/min and measurement carried out at UV 260 nm. The validation parameters namely linearity, sensitivity, selectivity, specificity. robustness, precision and accuracy were determined. The peak area response-concentration curve was linear, over the concentration range of 125-750 μg/mL for Metformin and 1.25-7.50 μg/mL for Empagliflozin with quantitation limits of 4.59 μg/mL for Metformin, and 0.08 μg/mL for Empagliflozin. The proposed method was successfully validated for the determination of Metformin and Empagliflozin simultaneously in combined tablet dosage form. The performance of the present method was compared with available RP-HPLC methods and found to be rapid and economical. The proposed stability-indicating RP-HPLC method can be successfully applied to quality control and drug analysis.Keywords
Metformin, Empagliflozin, RP-HPLC, ICH Guidelines, Stability-indicating method.References
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