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Rekha, A.
- Development of Normalized Cdna Library from Fusarium Wilt Infected Roots of a Tolerant Banana Genotype 'Calcutta-4' Musa acuminata ssp. burmannicoides
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Authors
Affiliations
1 Division of Biotechnology, ICAR - Indian Institute of Horticultural Research, Hesaraghatt Lake Post, Bengaluru - 560089, IN
2 Division of Fruit Crops, ICAR - Indian Institute of Horticultural Research, Bengaluru, IN
1 Division of Biotechnology, ICAR - Indian Institute of Horticultural Research, Hesaraghatt Lake Post, Bengaluru - 560089, IN
2 Division of Fruit Crops, ICAR - Indian Institute of Horticultural Research, Bengaluru, IN
Source
Journal of Horticultural Sciences, Vol 9, No 1 (2014), Pagination: 55-60Abstract
Management of the most devasting disease, Fusarium wilt of banana, caused by the fungus Fusarium oxysporum f. sp., cubense, is a challenge to the plant pathologist and the banana grower. Currently, genomics is providing the way for understanding plant defense mechanism, having acquired an important place in crop improvement. To identify the relevant genes and to understand the defense mechanism induced during Fusarium wilt infection, a normalized cDNA library was constructed from infected ischolar_main samples of a tolerant banana genotype, Musa acuminata spp. burmannicoides 'Calcutta-4', by duplex specific nuclease (DSN) based normalization, using the SMART (switching mechanism at 5' end of RNA transcript) full-length cDNA construction method. Sequencing and analysis of 600 clones revealed 392 non-redundant clones. In all, of 88% of the sequences were annotated using Musa genome database, and the remaining 12% were identified as novel loci not annotated. We observed several resistance genes, ROS scavenging genes and genes involved in ubiquitin-proteosome pathway in this study. These genes may have a possible role against Foc infection. These sequences would enrich the EST data developed against specific stress, which is an indispensable tool for predicting functional genes and understanding the defense mechanism.Keywords
Fusarium oxysporum f.sp. cubense, Banana, cDNA Library, Defense Response, Normalization.Full Text
- Genetic Correlation and Cluster Analysis in Sapota (Manilkara zapota)
Abstract Views :213 |
PDF Views:110
Authors
Affiliations
1 Division of Fruit Crops, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore – 560 089, IN
2 Section of Economics and Statistics, IN
1 Division of Fruit Crops, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore – 560 089, IN
2 Section of Economics and Statistics, IN
Source
Journal of Horticultural Sciences, Vol 6, No 2 (2011), Pagination: 101-104Abstract
Sapota is classified into two main types based on fruit shape as 'round' and 'oval'. However, there are several intermediates, as, it is a heterozygous tree crop. In this study, an effort was made to group available variability in sapota based on fruit characters and to analyze it. This helps in selection of parents for use in further breeding programmes. Cluster analysis revealed four definite clusters. High variability was observed for fresh-fruit weight, fruit length, fruit girth, fruit weight at ripening, pulp weight, peel weight, number of seeds and TSS. Correlation studies among fruit parameters indicated positive relationship between all the parameters studied, except TSS which had negative relationship with the rest of the fruit parameters. Selection of distantly placed cultivars in breeding programs stands to result in better progeny for further evaluation.Keywords
Sapota, Fruit Parameters, Variability, Correlation, Clusters.Full Text
- Chromosome Studies and Karyotype Analysis of some Triploid Banana (Musa Species) Cultivars of AAA Genomic Group
Abstract Views :248 |
PDF Views:144
Authors
A. Rekha
1,
S. C. Hiremath
2
Affiliations
1 Division of Fruit Crops, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore-560 089, IN
2 Department of Botany, Karnataka University, Dharwad - 580 003, IN
1 Division of Fruit Crops, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore-560 089, IN
2 Department of Botany, Karnataka University, Dharwad - 580 003, IN
Source
Journal of Horticultural Sciences, Vol 3, No 1 (2008), Pagination: 30-34Abstract
Bananas are the highly evolved, oldest fruits known to mankind. The Cavendish group cultivars are popular commercial varieties. AAA genomic group cultivars are said to have evolved from the wild AA Musa acuminata species by natural hybridization and polyploidization and these vigorous triploids were selected by man for cultivation. Basic cytological studies on banana are comparatively few due to the plant's complex nature. In this report, karyo-morphological studies on five AAA Cavendish group cultivars i.e. Robusta, Dwarf Cavendish, Grand Naine, Gros Michel and Red banana are reported. All the five cultivars had similar karyotype, except cv. Robusta. Total chromosome length was highest in Red banana and lowest in cv. Gros Michel.Keywords
Cavendish Banana, Triploid, Karyo-Morphology.Full Text
- Physiological and Biochemical Changes during Moisture Stress in Banana
Abstract Views :205 |
PDF Views:0
Authors
Affiliations
1 Department of Biotechnology, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), IN
2 Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru (Karnataka), IN
1 Department of Biotechnology, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), IN
2 Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru (Karnataka), IN
Source
Asian Journal of Bio Science, Vol 7, No 1 (2012), Pagination: 1-4Abstract
Present investigation was carried out to study the physiological and biochemical changes during moisture stress by identifying two contrasting genotypes from earliar studies i.e. M. acuminata. ssp burmaniceoides commonly recognised as 'Calcutta-4' belonging to AA genomic group and 'Bee hee kela' belonging to BB genomic group were used. The per cent reduction in photosynthetic rate (PN) between control and stressed plants was 45.28 (%) for 'Calcutta-4' and 36.01(%) for 'Bee hee kela', Transpiration rate (E) was 30.24 (%) for 'Calcutta-4' and 22.36 (%) for 'Bee hee kela' and Stomatal conductance (gs) was 60.30 (%) for Calcutta-4 and 56.10 (%) for 'Bee hee kela', indicating BB genotypes are tolerant to water deficit conditions. Leaf water potential (Ø) was higher in 'Bee hee kela' (BB) both in watered (-0.913 MPa) and under stress (-1.518 MPa) situations when compared with 'Calcutta-4' (AA) control (-1.35 Mpa) and stressed (-1.824 MPa) plants. Malondialdehyde content was estimated to be high in 'Calcutta-4' than that of 'Bee hee kela' indicating higher degree of membrane damage in Calcutta-4. The two antioxidant enzymes namely Super oxide dismutase (SOD) and Catalase activities were found to be higher in Bee hee kela stressed samples than 'Calcutta- 4' indicating better oxidative damage withstanding capacity. 'Bee hee kela' (BB) showed higher Ø, gs, and antioxidant enzyme (SOD and Catalase) activities. 'Bee hee kela' genotype is more drought tolerant than 'Calcutta-4'. Identified drought tolerant genotype can be further used as a donor for drought tolerance.Keywords
Banana, Drought, Photosynthesis, Melondialdehyde, Antioxidants.
- Eulophid Seed Borer, Anselmella kerrichi (Narayanan et al.; Hymenoptera), An Emerging Pest of Jamun
Abstract Views :314 |
PDF Views:75
Authors
Affiliations
1 Division of Entomology and Nematology, ICAR-Indian Institute of Horticultural Research, Hesseraghatta Lake PO, Bengaluru 560 089, IN
2 Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research, Hesseraghatta Lake PO, Bengaluru 560 089, IN
1 Division of Entomology and Nematology, ICAR-Indian Institute of Horticultural Research, Hesseraghatta Lake PO, Bengaluru 560 089, IN
2 Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research, Hesseraghatta Lake PO, Bengaluru 560 089, IN
Source
Current Science, Vol 117, No 6 (2019), Pagination: 922-924Abstract
Jamun, Syzygium cumini L. (Myrtaceae) is a common evergreen perennial fruit tree of tropical and subtropical regions. Being native to India and Myanmar, it is found throughout Southeast Asia and the Pacific regions1,2. The tree bears annually, sweetish and sour, dark-purple berries that are much sought after for their antioxidant, anti-inflammatory, anti-microbial and free-radical-scavenging properties3. The existing literature provides an exhaustive list of various insect pests (~78 species) that could infest S. cumini in India4. However, none of these studies reported seed borer, Anselmella kerrichi (Narayanan et al., 1958) (Hymenoptera: Eulophidae) as an insect pest of jamun.Full Text
References
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- Management of Eulophid Seed Borer, Anselmella kerrichi (Narayanan et al.) (Hymenoptera : Chalcidoidea : Eulophidae) on Jamun
Abstract Views :167 |
PDF Views:97
Authors
Affiliations
1 Division of Entomology and Nematology, ICAR- Indian Institute of Horticultural Research, Hesaraghatta Lake PO, Bengaluru - 560 089, IN
2 Division of Fruit crops, ICAR- Indian Institute of Horticultural Research, Hesaraghatta Lake PO, Bengaluru - 560 089, IN
1 Division of Entomology and Nematology, ICAR- Indian Institute of Horticultural Research, Hesaraghatta Lake PO, Bengaluru - 560 089, IN
2 Division of Fruit crops, ICAR- Indian Institute of Horticultural Research, Hesaraghatta Lake PO, Bengaluru - 560 089, IN
Source
Journal of Horticultural Sciences, Vol 14, No 2 (2019), Pagination: 166-168Abstract
A field experiment was conducted at ICAR-Indian Institute of Horticultural Research, Bengaluru during 2019 to evaluate certain insecticides and botanicals against jamun seed borer, Anselmella keriichi (Naryananet al.). The results revealed that the seed borer infestation was significantly low in λ-cyhalothrin (4.20%) and cypermethrin (5.77%) treatments followed by spinosad (6.36%), deltamethrin (6.40%) and imidacloprid (6.71%) (F=7.9; df=11; P<0.0001). Among the organic insecticides viz., spinosad @ 0.2 mL/L showed significant reduction in jamun seed borer infestation.Keywords
Syziumcumini, Chemical Insecticides, Botanical Insecticides, Hymenoptera and Insect Pest.Full Text
References
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- Do Conspecific Herbivores Track Resource Depletion through Host Phenology-Specific HIPVs?
Abstract Views :228 |
PDF Views:95
Authors
Affiliations
1 Division of Entomology and Nematology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bengaluru 560 089, India, IN
2 Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bengaluru 560 089, India, IN
1 Division of Entomology and Nematology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bengaluru 560 089, India, IN
2 Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bengaluru 560 089, India, IN
Source
Current Science, Vol 121, No 2 (2021), Pagination: 286-293Abstract
Conspecific herbivores use herbivore-induced plant volatiles (HIPVs) as cues while selecting an optimal site for oviposition. This is to ascertain the availability of nourishment for their progeny so that they get the best chance at survival. In the present study, phytophagous eulophid seed-borer Anselmella kerrichi was significantly (time spent: F3,40 = 13.12, P < 0.0001; number of entries: F2,30 = 4.21; P = 0.02) attracted to HIPVs from immature fruits of Java plum, Syzygium cuminii (time spent: 4.77 ± 0.40 min; number of entries: 2.27 ± 0.24) when also given the choice of mature fruits (time spent: 1.76 ± 0.32 min; number of entries: 1.46 ± 0.16), indicating that herbivores can assess re-source depletion from host phenology-specific HIPVs. The chemical cues like α-pinene, β-pinene, (z)-ocimene, undecane, 3,7-dimethyl decane, neo-allo-ocimene, allo-ocimene, ethyl benzoate, 2,6,11-trime¬thyldodecane, α-copaene and β-caryophyllene, which are present in immature fruit volatiles elicited antennal response in a GC-EAD analysis. Olfactometer analyses with the synthetic compounds also revealed that A. kerrichi was significantly attracted to these cues and the synthetic blend composed of the above compounds proved to be much more efficient in attracting female wasps when compared to a natural blend. Field evaluations using the synthetic blend showed that it could attract a significant number of A. kerrichi, indicating the scope of using this blend of synthetic HIPVs as a sustainable IPM toolKeywords
Anselmella kerrichi, Herbivore-Induced Plant Volatiles, Host Phenology, Resource Depletion, Syzygium cuminii.Full Text
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