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Gopi Reddy, A.
- Evaluation of Anticancer Activity of Ethanolic and Ethylacetoacetate Extracts of Sweet Cherry against Human Breast Cancer Cell Line MCF-7
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Authors
Affiliations
1 Department of Pharmaceutical Chemistry, Teja College of Pharmacy, Kodad, Nalgonda-508206, Telangana State, IN
2 Department of Pharmaceutical Chemistry, SANA College of Pharmacy, Kodad, Nalgonda-508206, Telangana State, IN
1 Department of Pharmaceutical Chemistry, Teja College of Pharmacy, Kodad, Nalgonda-508206, Telangana State, IN
2 Department of Pharmaceutical Chemistry, SANA College of Pharmacy, Kodad, Nalgonda-508206, Telangana State, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 8, No 2 (2016), Pagination: 65-70Abstract
MCF-7 is a breast cancer cell line isolated in 1970 from a 69-year-old Caucasian woman. MCF-7 is the acronym of Michigan Cancer Foundation-7, referring to the institute in Detroit where the cell line was established in 1973 by Herbert Soule and co-workers. The MCF7 line retains several characteristics of differentiated mammary epithelium including ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes. The cells express the WNT7B oncogene. Growth of MCF7 cells is inhibited by tumor necrosis factor alpha (TNF alpha). Secretion of IGFBP's can be modulated by treatment with anti-estrogens. PIK3CA helical mutations were identified in MCF-7, but with low AKT activation. The main objective of the present research work is to isolate the bioactive molecules and evaluate the in vitro anticancer activity of ethanolic and ethyl acetoacetate extracts of sweet cherry (EEC and EAAEC) of Prunus avium. The in vitro anticancer activity was carried out against human breast cancer cell line MCF-7 by MTT assay. The results obtained from the in-vitro studies performed by MTT assay by using human breast cancer cell line MCF-7 displayed that the various extracts of sweet cherry (EEC and EAAEC) possessed a very good anticancer activity. From the present studied it had been concluded that EEC and EAAEC, all were exhibiting the potential capability to inhibit the cancer cell when compared with standard drug doxorubicin and the cell growth inhibition of EEC and EAAEC was found to be the highest 92.90% growth inhibition at 10 μg (IC50 = 2.4 μg/ml) and EAAEC with the 92.49% growth inhibition at 10 μg (IC50 = 2.9 μg/ml).Keywords
MCF-7, WNT7B Oncogene, TNF Alpha, anticancer, MTT Assay, IC50 etc.
- Extraction and Isolation of Bioactive Molecule Lycopene from Water Melon and Evaluation of Anti Diabetic Activity Against STZ Induced Rats
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Authors
Affiliations
1 Department of Pharmacy, Meera Bai Institute of Technology, Maharani Bagh, New Delhi-110065., IN
2 Department of Pharmaceutical Chemistry, Teja College of Pharmacy, Kodad, Nalgonda-508206, Telangana State, IN
3 Department of Pharmaceutical Chemistry, Sana College of Pharmacy, Kodad, Nalgonda-508206, Telangana State, IN
4 Department of Pharmacology, Avanthi Institute of Pharmaceutical Sciences, Guthapally, Hyderabad-501512, IN
5 Department of Pharmaceutical Analysis and Quality Assurance, Avanthi Institute of Pharmaceutical Sciences, Guthapally, Hyderabad-501512, IN
1 Department of Pharmacy, Meera Bai Institute of Technology, Maharani Bagh, New Delhi-110065., IN
2 Department of Pharmaceutical Chemistry, Teja College of Pharmacy, Kodad, Nalgonda-508206, Telangana State, IN
3 Department of Pharmaceutical Chemistry, Sana College of Pharmacy, Kodad, Nalgonda-508206, Telangana State, IN
4 Department of Pharmacology, Avanthi Institute of Pharmaceutical Sciences, Guthapally, Hyderabad-501512, IN
5 Department of Pharmaceutical Analysis and Quality Assurance, Avanthi Institute of Pharmaceutical Sciences, Guthapally, Hyderabad-501512, IN
Source
Research Journal of Pharmacy and Technology, Vol 11, No 1 (2018), Pagination: 101-106Abstract
Despite the popular belief that watermelon is just water and sugar, watermelon is actually a nutrient dense food. It provides high levels of vitamins, minerals, and antioxidants and just a small number of calories. Water melons have become synonymous with summer and picnics, and for good reason. Their refreshing quality and sweet taste help to combat the heat and provide a guilt-free, low maintenance dessert. Along with cantaloupe and honeydew, watermelons are a member of the botanical family Cucurbitaceae. There are five common types of watermelon: seeded, seedless, mini (also known as personal), yellow, and orange. The objective of the present research work was to evaluate in vivo anti diabetic activity of ethanolic extract of water melon (EE-WMN).The present experimental data displayed the blood glucose level 283.5±0.4238–284.2±0.02217 mg/dL mg/dL in STZ induced diabetic rats, which was considered as severe diabetes in the glibenclamide (5 mg/kg) and EE-WMN (100, 200 and 300 mg/kg) treated groups, the peak values of blood sugar and cholesterol and triglycerides significantly reduced on the 21st day, respectively. Hence, this experimental data displayed that the EE-WMN significantly decrease the blood glucose level in diabetic rats but values did not return to those of normal controls. Therefore, the EE- WMN executed anti diabetic activity, when compared with diabetic control. From the present experimental data, here we concluded that EE-WMN displayed potential anti diabetic activity against streptozocin induced rats at 200 and 300 mg/kg. b. wt which was proved by assessment of bioanalytes from serum and urine sample and this anti diabetic activity was mainly due to the presence of carotenoids lycopene which was characterized by UV, FT-IR, 1H and 13C-NMR, APCI, HPLC spectrometry.Keywords
Anti Diabetic, STZ, Triglycerides, FT-IR and APCI etcReferences
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