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G. Vaijayanthi ¹, A. Cholarajan ², R. Vijayakumar ³

Affiliations
1 P.G. Department of Microbiology, Meenakshi Chandrasekaran College of Arts and Science, Pattukkottai – 614 626, Tamil Nadu, IN
2 P.G. Department of Microbiology, Meenakshi Chandrasekaran College of Arts and Science Pattukkottai-614 626, Tamil Nadu, IN
3 Department of Microbiology, Bharathidasan University College for Women, Orathanadu-614 625, Tamil Nadu, IN

Abstract

The terrestrial soil sample was collected from Orathanadu, Thanjavur (Dt.), Tamilnadu, India. Physical and chemical characteristics of soil samples such as pH, electrical conductivity (EC), calcium (Ca2+), magnesium (Mg2+), bicarbonate (HCO3^-), chloride (Cl^-), total organic carbon, available nitrogen (N), phosphorus (P₂O₅) and potassium (K₂O) were analyzed. A total of 10 actinobacteria were isolated from the terrestrial soil sample and they were characterized based on the morphological and biochemical properties. Among 10 isolates, each one was belonged to Actinobispora sp., Actinosynnema sp., Actinoplanes sp., Agromyces sp., Actinomadura sp., Jonesia sp., Catellatospora sp., Micromonospora sp., Microtetrospora sp. and Nocardia sp. Antibacterial activity of actinobacteria were tested against five different human pathogenic bacteria such as Salmonella typhi, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Enterobacter aerogenes. Further, the purification of antibacterial compound was performed by using thin layer chromatography and the functional groups of bioactive compounds were determined by FT-IR and UV spectral studies.

Keywords

Terrestrial Soil, Actinobacteria, Physico-Chemical Analysis, Antibacterial Activity, Spectral Studies.

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K. Kaviyarasi ¹, A. Cholarajan ², R. Bharathidasan ², P. Madhanraj ², A. Panneerselvam ²

Affiliations
1 P.G. and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi -613503, Thanjavur, Tamilnadu, IN
2 P.G. and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi -613503, Thanjavur, Tamilnadu, IN

Abstract

PGPR offers an environmentally sustainable approach to crop production and health. Many members of genera pseudomonas sp. perform beneficial effects on plants. Among them P. putida and P. fluorescens are fluorescent pseudomonas that represent Significant part of these Members. The rhizosphere soil was collected from cultivated plots. P. fluorescence was isolated and identified through morphologicaland hemical tests and it was treated against some plant pathogens. The biocontrol potential of P. fluorescens against plant pathogen such as A. terreus sp, Circinella sp, Verticillium sp, was assessed by dual plate technique. The seed treatment was made on four plants namely Hibiscus esculentus, Oryza sativa, Capsicum annum, Spinacia olerace. Pot culture techniques was applied for the growth of plants along with p. fluorescence inoculums (100ml) and chemical fertilizer (10g).The plant height, ischolar_main length, ischolar_main biomass, shoot biomass were also estimated. The results were discussed.

Keywords

PGPR, Pseudomonas fluorescence.

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R. Malathi ¹, G. Melchias ², S. Chandrasekar ¹, A. Cholarajan ³

Affiliations
1 Department of Biotechnology, Bharathidasan University College, Perambalur-621 212, IN
2 Department of Biotechnology, St. Joseph College, Trichy –620 002, IN
3 P.G. Department of Microbiology, Meenakshi Chandrasekaran College of Arts and Science, Pattukkottai – 614 626, Tamilnadu,, IN

Abstract

The role of aluminium and phosphorus on the secretion of acid phosphatase in the callus culture of Brassia juncea was studied. Callus culture derived from leaves of mustard (Brassica juncea) plants. Proliferated calluses were subjected to P-deficient as well as P-sufficient conditions in the presence and absence of aluminium. The 10 days old calluses were harvested and so the culture medium in which they were cultured, for analysis of acid phosphatase, protein and amino acid content.

Keywords

Tissue Culture, Brassica juncea, Callus.

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R. Malathi ¹, A. Cholarajan ², K. Karpagam ¹, K. R. Jaya ¹, P. Muthukumaran ³

Affiliations
1 Department of Biotechnology, Bharathidasan University College (w), Orathanadu, Thanjavur, Tamilnadu, IN
2 Department of Microbiology, Meenakshi Chandrasekaran College of Arts and Science, Pattukkottai – 614 626, Thanjavur, Tamilnadu, IN
3 Department of Biochemistry, Meenakshi Chandrasekaran College of Arts and Science, Pattukkottai – 614 626, Thanjavur, Tamilnadu, IN

Abstract

Medicinal plants contribute in human health care system. Most of the plants utilizes by village people as a folk medicine. Now we are turned in to medicinal plant analysis of active compounds and conservation aspect. In the present study we had select the three important medicinal plants. We have collected three medicinally important medicinal plants such as Coleus amboinicus, Phyla nodiflora and Vitex negundo for antimicrobial studies. The experiment carried out in the selected medicinal plants leaves. The results are discussed with the available literature.

Keywords

Antimicrobial Activity, Pathogens, Coleus amboinicus, Phyla Nodiflora and Vitex Negundo.

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R. Malathi ¹, S. Ahamed John ², A. Cholarajan ³

Affiliations
1 Department of Biotechnology, Bharathidasan University College (W), Orathanadu, Thanjavur, Tamil Nadu, IN
2 Department of Botany, Jamal Mohamed College, Trichy, Tamil Nadu, IN
3 Department of Microbiology, Meenakshi Chandrasekaran College of Arts and Science, Pattukkottai – 614 626, Thanjavur, Tamil Nadu, IN

Abstract

Extract from the leaves of Tylophora asthmatica were investigated for antioxidant activity. The methanolic extract of T.asthmatica had a DPPH scavenging activity of 84.6% at 250μg/ml and a reductive potential of 0.77 at 100μg/ml. These values were comparable with those of Gallic acid, 91.4% at 250μg/ml and Ascorbic acid, 0.79 at 60μg/ml as standards for DPPH scavenging activity and reductive potential, respectively. These findings suggest that the rich phytochemical content of T.asthmatica and its good antioxidant activity may be responsible for its popular and wide traditional use. The experiment was carried out with the leaves of the selected medicinal plants. The results are discussed with the available literature.

Keywords

Tylophora asthmatica, Antioxidant Activity, Reductive Potential, DPPH.

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R. Malathi ¹, S. Ahamed John ², A. Cholarajan ³

Affiliations
1 Department of Biotechnology, Bharathidasan University College (w), Orathanadu, Thanjavur, Tamilnadu, IN
2 Department of Botany, Jamal Mohamed College, Trichy, Tamilnadu, IN
3 Department of Microbiology, Meenakshi Chandrasekaran College of Arts and Science, Pattukkottai – 614 626, Thanjavur, Tamilnadu, IN

Abstract

Methanolic extract of Tylophora asthmatica leaves were prepared and tested for its antilipidperoxidation effect against acetaminophen induced hepato toxicity in rats. Alteration in the levels of lipid peroxide, iron, ferritin, total cholesterol, total lipid and phospholipids were tested in both experiment and control group of rats. Acetaminophen [1gm/kg] has showed a significant increase [p<0.05] in experimental rats except the level of phospholipids, which showed a significant decrease [p<0.05] compared to control rats. Treatment with methanolic extract of Tylophora asthmatica leaves [200mg/kg] has brought back the altered levels of this parameter to the near normal levels in the dose dependent manner.

Keywords

Acetaminophen, Hepatoprotective, Tylophora asthmatica.

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J. Kasthuri ¹, A. Cholarajan ¹, R. Vijayakumar ², P. Muthukumaran ³

Affiliations
1 P.G Department of Microbiology, Meenakshi Chandrasekaran College of Arts and Science Karambayam - 614 626, Thanjavur Dt., Tamilnadu, IN
2 Department of Microbiology, Bharathidasan University College for Women, Orathanadu-614 625, Thanjavur District, Tamil Nadu, IN
3 P.G Department of Biochemistry, Meenakshi Chandrasekaran College of Arts and Science ,Karambayam - 614 626, Thanjavur Dt., Tamilnadu, IN

Abstract

The coir industry is one of the major agro-based industries contributing significantly to the national income. This work deals with the characteristics of the waste water discharged from the coir industry. The results presented here are the physical, chemical and microbiological analysis of the waste water samples collected from the coir industry. The analysis indicates the Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD) and nutrient such as nitrate, nitrite, ammonia, calcium and magnesium were recorded high amount and a microbial populations including bacteria, fungi and algae in the waste water.

Keywords

Coir Pith, Physico-Chemical Properties, Bacteria, Fungi, Algae.

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