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A. S. Goswami-Giri ¹, Neha A. Sawant ¹

Affiliations
1 B.N. Bandodkar College of Science, Thane-400 601, IN

Abstract

Bioactive molecules of onion leaves exhibited potent anti polyphenol oxidase (PPO) activity; which was purified on silica gel by using solvents. Purification profile procured 3 and 2 active butanol and methanol fractions respectively. Reticence of PPO activity was observed within range of 12 to 14 min when it was treated with active fractions. Characterization of it was conceded by physical constant, UV, IR and TLC. It reputes that, active hydroxyl group attached to the ring structures of PPO, which quench free radical by donating hydrogen in the active hydroxyl group of PPO, confirming inhibition of pigmentation reaction in vitro. Bioactive molecules from onion leaves exhibited various types of inhibition against oxidation of L-DOPA and L-tyrosine and it was compared with classical inhibitors of PPO. Significance of bioactive molecules is to explore for drugs designing, understanding of ligand-receptor interactions, environmental problems and it is simple alternative for the inhibition of PPO.

Keywords

Onion Leaves, Quercetin, Oxazolone, Flavanoid, Cinnamic Acid, Inhibition.

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Saiprabha M. Mahale ¹, A. S. Goswami-Giri ¹

Affiliations
1 Department of Chemistry, B. N. Bandodkar College of Science, Chendani Bunder Road, Thane – 400 601, Maharashtra, IN

Abstract

Crude and refined seed kernel oil was extracted from Alphanso mango aiming the study of physico-chemical properties. The oil produced was refined through degumming, neutralization and bleaching process using local adsorbent (activated clay). The specific gravity, refractive index, acid value, saponification value and iodine value for both crude and refined mango seed kernel oil was determined. The solid form refined mango kernel fat resembles cocoa butter when compared with physical and chemical characteristics. They both are thus, used as a substitute for each other or just as extenders. Mango Fat can be used as edible oil apart from its general uses in manufacturing soap, cosmetic formulations and industrial applications.

Keywords

Seed Kernel, Mango, Oil, Soxhlet Extraction, Refining, Degumming, Bleaching etc.

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