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Authors
Affiliations
1 Department of Chemistry & Biochemistry, CCS Haryana Agricultural University, Hisar, Haryana, IN
2 Department of GPB, CoA, CCS Haryana Agricultural University, Hisar, Haryana, IN
Source
International Journal of Science, Engineering and Computer Technology, Vol 6, No 2 (2016), Pagination: 155-157
Abstract
In the present investigation, two varieties viz. DH-5 and DH-36 of Coriandrum sativum leaves were assayed for their phytochemical profile using different solvents and at different temperature namely, water, hot water(100oC) and methanol. These extracts were also evaluated for antibacterial activity by well diffusion method. The results revealed that among the two varieties; variety DH-5 contained higher, flavonoids, chlorophyll a and chlorophyll b contents in comparison to variety DH-36. Amongst the different extracts, methanol extract of both varieties contained highest flavonoids content. The antibacterial activities of different solvent were tested against the bacterial strains Micrococcus luteus (Gram-positive) and Escherichia coli (Gram negative) by observing the zone of inhibition. The methanol extract of Coriandrum sativum leaves extracts of variety DH-5 showed more activity against E.coli, zone of diameter 18.21 ± 0.35 mm, water extract showed more activity against E.coli (16.40 ± 0.70 mm) and methanol extract of variety DH-36 showed more activity against E.coli (15.23 ± 0.10 mm), water extract showed more activity against E.coli (14.38 ± 0.86 mm).
Keywords
Coriandrum sativum, Flavonoids, Chlorophyll A, Chlorophyll B, Antibacterial Activity.
