Indian Journal of Public Health Research & Development

Heri Irawan ¹, Romziah Sidik ¹, Tri Nurhajati ¹, Muchammad Yunus ¹, Arif Nur Muhammad Ansori ¹, Muhammad Khaliim Jati Kusala ¹, Naimah Putri ¹

Affiliations
1 Universitas Airlangga, Kampus C Universitas Airlangga, 60115, Surabaya, ID

Abstract

The aim of this study was to examine the effect of feeding using complete feed added with various formula toward the level of milk’s palmitic and palmitoleic acids in Holstein-Friesian cross cow. In this study, we used ten female Holstein-Friesian cross cows between four and seven years with two to six lactation periods. The substances were control feed P0 and complete feed with different formula P1, P2, P3, P4. The study was completely randomized design with five treatments and two replications. The data were analyzed using analysis of variance and the differences means between of the treatments were tested by Duncan’s multiple range tests. The software that was used to analyze the data was windows Statistical Program of Social Science 22 (SPSS 22). Interestingly, the result of the study showed that P3 complete feed containing the lowest crude fiber among the treatments could increase the levels of palmitic and palmitoleic acids in the milk of cow. In conclusion, fed dairy cows using complete feed with a different formula affect the levels of palmitic and palmitoleic acids in cow’s milk significantly.

Keywords

Palmitic Acid, Palmitoleic Acid, Complete Feed, Milk, Holstein-Friesian.

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Naimah Putri ¹, Rahaju Ernawati ², Suwarno ², Kusnoto ³, Wiwiek Tyasningsih ⁴, Fedik Abdul Rantam ⁵

Affiliations
1 Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115, ID
2 Laboratory of Virology and Immunology Department Microbiology, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115,, ID
3 Laboratory of Parasitology Department Parasitology, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115,, ID
4 Laboratory of Bacteriology and Micology Department Microbiology, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115, ID
5 Stem Cell Research and Development Center, Universitas Airlangga, ID

Abstract

This study were conducted to analyze the homology and the relation of nucleotidesencoding partial genomic sequence of Fusion (F) protein Newcastle Disease Virus (NDV) isolated from swanusing phylogenetic tree. Samples were collected from cloacal swabs of seventy one waterfowls. Consisting of twenty four Domestic swan, thirty three Muscovy ducks, and fourteen Domestic ducks form waterfowl in East Java Province, and also positive LaSota isolate (ATCC). Samples were isolated in embryonated chicken eggs(ECEs) with specific pathogen free (SPF)and identified by HA test and confirmed as NDV by HI test. Positive samples were performed by RT-PCR using a forward and reverse primer with a target of 699 bp.The nucleotide sequences from field isolates were compared with the gene of vaccine viruses isolates encoded F protein NDV that used in Indonesia and vaccine viruses encoded F protein NDV that spread across the globe obtained at GenBank data and had very close phylogenetic relation with those vaccine viruses LaSota.Analysis of homology and phylogenetic from isolates in this study relate to isolate of vaccine viruses.

Keywords

Newcastle Disease Virus, F Protein, Homology, Phylogenetic Tree, RT-PCR.

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