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Amaldas, Julius
- Artemesia Annua L. Nanoparticles Destabilize Membrane Integrity and Induce Apoptosis in a Caspase Mediated Pathway in MDA-MB-231 Cells
Authors
1 Department of Biochemistry, Bharathiar University, Coimbatore, Tamil Nadu, IN
2 Department of Biochemistry, Sree Balaji Dental College & Hospital, BIHER, Tamil Nadu, IN
Source
Indian Journal of Public Health Research & Development, Vol 10, No 12 (2019), Pagination: 923-927Abstract
Breast cancer exhibits diagnostic and therapeutic challenges due to tumour heterogenicity and poor efficacy of anti cancer drugs at the metastatic sites. Recently, nanomedicines provide a potential benefit to breast cancer treatment by their increased bioavailability, selective targeting, increased efficacy and lower toxicity. This work aimed to evaluate the cytotoxic potential of A. annua silver nanoparticles (AGNPs) on human triple-negative breast cancer cell lines. The mechanism by which A.annua AGNPs exhibit growth inhibition was analysed by using the status of LDH, Mitochondria Membrane potential [MMP], and ATP levels. Finally, the ability of A. annua AGNPs to mediate cell death was studied by protein expressions of Bax, BCL-2, Caspase 3and Caspase 9. We observed that A. annua AGNPs show a remarkable cytotoxic effect by increasing levels of LDH with loss of MMP and reduced ATP levels. Here in this study, protein expression of Bax, BCL-2 ratio, Cyt C and Caspase 9 protein expression level favors apoptotic induction during AGNPs treatment. Henceforth we prove that A.annua AGNPs can be useful as alleviative regiment against breast cancer and can be explored for other cancers and its related pathologies.Keywords
Breast Cancer, Bax, Bcl-2, Apoptosis, Mitochondria, Caspase 9.
- Oral Mucosal Lesions among Tobacco Users in Chennai, Tamil Nadu:A cross sectional study
Authors
1 Department of Oral Medicine and Radiology, Sree Balaji Dental College and Hospital, BIHER, Pallikaranai, Chennai, Tamil Nadu, IN
2 Department of Conservative Dentistry and Endodontics, Sathyabama Dental College and Hospital, Semmanchery, Chennai, Tamil Nadu, IN
3 Department of Orthodontics, Sree Balaji Dental College and Hospital, BIHER, Pallikaranai, Chennai, Tamil Nadu, IN
4 Department of Biochemistry, Sree Balaji Dental College and Hospital, BIHER, Pallikaranai, Chennai, Tamil Nadu, IN
5 Sree Balaji Dental College and Hospital, BIHER, Pallikaranai, Chennai, Tamil Nadu, IN
Source
Indian Journal of Public Health Research & Development, Vol 10, No 12 (2019), Pagination: 1026-1030Abstract
Background: Oral cancer is eighth most common cancer worldwide. They may arise from potentially malignant disorders1. The etiology of Oral cancer is multifactorial, the most common among those are the effects of tobacco usage7. The tobacco is used in various forms in our country Tobacco chewing and smoking are associated with oral mucosal lesions such as Leukoplakia, Oral submucous Fibrosis which are potentially malignant lesions9. This study was aimed at identifying the prevalence of these oral mucosal lesions and to motivate the tobacco users about the various deleterious effects associated with tobacco and to help them to quit their habits.
Aim: The aim of this study is to determine the prevalence of oral mucosal lesions among the tobacco users in Chennai.
Materials and Method: The study sample was randomly selected and comprised 1483 patients attending the Department of Oral Medicine and Radiology, Sree Balaji Dental College and hospital, Chennai between January 2017 and December 2018. The ages ranged from 18 to 65years and are tobacco users were eligible to participate in the study. The data collected was tabulated in Microsoft Excel and further analysed using SPSS Version 21.0(IBM), software for statistical analysis.
Results: A total of 1483 patients were examined to diagnose the lesions associated with the use of tobacco. The most frequently seen lesion was Smokers Melanosis [921(62.1%)]followed by Frictional Keratosis [696(46.9%)],Periodontal Inflammation [551(37.2%)], Leukoedema [304(20.5%)] and Stomatitis Nicotina Palatini [160(10.8%)].
Conclusion: The overall prevalence of potentially malignant disorders have slight variations when compared to other studies. This may be due to difference in habits, duration and frequency of use.