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Authors
Affiliations
1 Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115, ID
2 Laboratory of Virology and Immunology Department Microbiology, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115,, ID
3 Laboratory of Parasitology Department Parasitology, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115,, ID
4 Laboratory of Bacteriology and Micology Department Microbiology, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115, ID
5 Stem Cell Research and Development Center, Universitas Airlangga, ID
Source
Indian Journal of Public Health Research & Development, Vol 10, No 11 (2019), Pagination: 1895-1899
Abstract
This study were conducted to analyze the homology and the relation of nucleotidesencoding partial genomic sequence of Fusion (F) protein Newcastle Disease Virus (NDV) isolated from swanusing phylogenetic tree. Samples were collected from cloacal swabs of seventy one waterfowls. Consisting of twenty four Domestic swan, thirty three Muscovy ducks, and fourteen Domestic ducks form waterfowl in East Java Province, and also positive LaSota isolate (ATCC). Samples were isolated in embryonated chicken eggs(ECEs) with specific pathogen free (SPF)and identified by HA test and confirmed as NDV by HI test. Positive samples were performed by RT-PCR using a forward and reverse primer with a target of 699 bp.The nucleotide sequences from field isolates were compared with the gene of vaccine viruses isolates encoded F protein NDV that used in Indonesia and vaccine viruses encoded F protein NDV that spread across the globe obtained at GenBank data and had very close phylogenetic relation with those vaccine viruses LaSota.Analysis of homology and phylogenetic from isolates in this study relate to isolate of vaccine viruses.
Keywords
Newcastle Disease Virus, F Protein, Homology, Phylogenetic Tree, RT-PCR.
