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Ankola, Anil V.
- Expression of Perlecan (Heparan Sulphate Proteoglycan) in Oral Squamous Cell Carcinoma
Authors
1 Department of Oral Pathology, KLE VK Institute of Dental Sciences, Belgaum, IN
2 Department of Oral Pathology, ITS Dental College & Hospital, Noida, IN
3 Department of Pathology, JN Medical College, Belgaum, IN
4 Department of Public Health Dentistry, KLE VK Institute of Dental Sciences, Belgaum, IN
Source
Indian Journal of Public Health Research & Development, Vol 3, No 1 (2012), Pagination: 185-188Abstract
Background
Perlecan is a basement membrane heparan sulfate proteoglycan. It plays a vital role in cell- cell adhesion, cell-matrix adhesion and is associated with several growth factors. Recently its role has been found in many pathological and physiological conditions. Aim of this study was to understand the immunolocalisation of perlecan in normal mucosa and oral squamous cell carcinoma and also to derive a correlation between perlecan expression and various grades of carcinoma.
Method
A total of thirty tissue blocks comprising of ten normal mucosa and twenty oral squamous cell carcinoma were included in the study. They were examined for the presence of perlecan protein core by immunohistochemistry using monoclonal antibody (Anti-Basement Membrane-type heparan sulfate proteoglycan core protein. Specificity: Mouse monoclonal anti human Perlecan Clone No: 85-9). Interpretation of staining was done and the observations statisticaly analysed by Fisher exact tests to evaluate the expression of perlecan within and around the tumor islands.
Results
In normal epithelium perlecan expression was limited to basal layer. In oral squamous cell carcinoma, perlecan was present in surface epithelium, stroma as well as tumor islands. Perlecan expression within tumor islands became scarce with the higher grades of carcinoma.
Conclusions
It was deduced from the results that with the increase in degree of carcinoma; more heparanase enzyme acts on perlecan and from the breakdown of perlecan; fibroblast growth factor, transforming growth factor-β and other growth factors are released. All these factors are known to promote tumor growth.
Keywords
Perlecan, Immunohistochemistry, Heparan Sulphate Proteoglycan, Oral Cancer, Growth Factors, Squamous Cell CarcinomaReferences
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- Effect of Two Tongue Cleaning Methods on Oral Mutans Streptococci Level
Authors
1 Department of Preventive and Community Dentistry, Bapuji Dental College and Hospital, Davangere-577004, IN
2 Department of Preventive and Community Dentistry, KLES’s Institute of dental sciences, Nehru Nagar, Belgaum, IN
3 Professor, Department of Forensic Medicine & Toxicology, S.S. Institute of Medical Sciences & Research Centre, Davangere-577005, Karnataka, IN
Source
Indian Journal of Public Health Research & Development, Vol 3, No 2 (2012), Pagination: 68-71Abstract
Background
Tongue scraping and brushing have been appreciated for hundreds of years but are still appreciated or used by the public. Scientific evidence has validated the need to practice habitual and tongue cleaning as part of daily home oral hygiene procedures.
Objective
To assess and compare the effect of tongue scraping and tongue brushing on oral Mutans streptococci level.
Methods
20 healthy subjects aged 14 to 15 years were randomly selected. Flat plastic tongue scraper and Nylon multitufted small headed tooth brush are the two tongue cleaning devise used. Unstimulated salivary samples were obtained at4 intervals from each individual. Salivary samples were inoculated on Mitis Salivary Agar Plate and Sorbital Broth was used for identification of Mutans streptococci group.
Results
Paired and unpaired 't' test were employed. Reduiction in the Mutans streptococci level from 48.4X104 CFU and 38.3X104 CFU at baseline in tongue scraping and tongue brushing group respectively to 0.34X104 and 0.39X104 CFU after 7th day.
Conclusion
Both tongue coating removal methods evaluated were efficient in reducing Mutans streptococci level. This implies that physical removal of the coating on the dorsum of the tongue is important and not the method used for the same.
Keywords
Tongue Scraping, Tongue Brushing, Tongue Cleaning, Mutans StreptococciReferences
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