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Gopalakrishnan, S.
- Antimicrobial Activity of Silver Nanoparticles from Swietenia Mahagoni
Authors
1 Department of Medical Biochemistry, University of Madras, Chennai-600113, IN
2 Department of Biochemistry JBAS College, Chennai 6000 018, IN
3 CLRI, Chennai 600020, IN
4 PNG University of Technology, PG
5 Department of Plant Biology and Plant Biotechnology, Presidency College, Chennai-600005, IN
Source
Indian Journal of Medicine and Healthcare, Vol 3, No 1 (2014), Pagination: 310-313Abstract
Objective: To produce natural, non-toxic biologically synthesized silver nanoparticles (Ag NPs) with antimicrobial activity from plant source, Swietenia mahagoni as a remedy for multi-resistant drug microbes.
Methods: The antimicrobial activity of these Ag NPs from Swietenia mahagoni was tested against three bacterial species, E.coli, S. aureus, P. aeruginosa and a fungal species, C. albicans.
Results: The experimental results exhibited satisfactory inhibitions against all tested microorganisms when used at different concentrations viz., 1mM, 3 mM and 5 mM; 3 mM (20 mg/500 μl distilled water) offered the highest sensitivity for E.coli and P. aeruginosa.
Conclusion: Green-synthesized antibacterial agent from Swietenia mahagoni showed good antibacterial and antifungal activity.
Keywords
E. Coli, C. Albicans , P. Aeuruginosa, Pithecellobium Dulce, S. AureusFull Text
- Comparison of Antimicrobial Activities of Silver Nanoparticles Synthesized from Dysoxylum parasiticum
Authors
1 The Papua New Guinea University of Technology, Lae, Morobe Province, Private Mail Bag., PG
2 University of Madras, Taramani Campus, Chennai-600113, IN
3 JBAS College for Women, Teynampet, Chennai-600018, IN
Source
Indian Journal of Medicine and Healthcare, Vol 4, No 2 (2015), Pagination: 1-5Abstract
Background/Objectives: In current trend biological synthesis of metallic nanoparticles is gaining importance because it is reliable and eco-friendly.
Methods/Statistical analysis: The present work leads to the synthesis of nanoparticles from 3mM and 5mM AgNO3 solution through aqueous leaf extract of Dysoxylum parasiticum as reducing as well as capping agent by employing boiling method and microwave irradiation method. Synthesized nanoparticles are characterized under UV-Vis spectroscopy at the range of 200-800nm.
Findings: Green synthesized silver nanoparticle showed zone of inhibition against tested microorganisms. The leaf extract shows higher antimicrobial activity found by disc diffusion method. Furthermore, determination of elemental silver was done by the Atomic Absorption Spectroscopy (AAS). The phytochemical constituent determination shows the positive results expect for the test for terpenoids and test for the reducing sugar.
Improvements/Application: Both Boiling Method and Microwave Irradiation Method are effective in synthesizing of silver nanoparticles using the leaves extracts of plants.
Keywords
Dysoxylum parasiticum, Antimicrobial Activity, Silver Nanoparticles, Silver Nitrate, Microwave Irradiation and Boiling Method.Full Text
- Comparison of Antimicrobial Activities of Silver Nanoparticles Synthesized from Sphaerostephanos asplenioides J. Sm
Authors
1 The Papua New Guinea University of Technology, Lae, Morobe Province, Private Mail Bag, PG
2 University of Madras, Taramani Campus, Chennai-600113, IN
3 JBAS College for Women, Teynampet, Chennai-600018, IN
Source
Indian Journal of Medicine and Healthcare, Vol 4, No 2 (2015), Pagination: 1-6Abstract
Background/Objectives: Green nanotechnology is generating attention of researchers toward ecofriendly biosynthesis of nanoparticles. In this study, the possible role of Sphaerostephanos asplenioides J. Sm extract in reducing silver nitrate into silver nanoparticle is highlighted.
Methods/Statistical analysis: A comparative study was made in preparing the silver nanoparticles using the boiling method and microwave irradiation method for the S. asplenioides. The synthesis of silver nanoparticles were prepared by adding silver nitrate solution [3mM] and [5mM] to the plant extract. The silver nanoparticles were characterized using the UV-Visible Spectroscopy.
Findings: The antimicrobial assay was carried out using the disc diffusion method which showed promising antibacterial effects against the 15 tested microorganisms. The phytochemical constituent determination shows the positive results expect for the test for terpenoids and test for the reducing sugar.
Improvements/Application: It can be concluded that both methods (Boiling Method and Microwave Irradiation Method) are effective in synthesizing of silver nanoparticles using the leaves extracts of plants.
Keywords
Sphaerostephanos asplenioides J. Sm., Antimicrobial Activity, Silver Nanoparticles, Silver Nitrate, Microwave Irradiation And Boiling Method.Full Text