V. Veerabadran ¹, S. Nithya Balasundari ², D. Monica Devi ², D. J. Mukesh Kumar ³

Affiliations
1 Department of Plant Biology and Plant Biotechnology, Loganatha Narayanasamy Government College, Ponneri, TN, IN
2 Department of Biotechnology, KalasalingamUniversity, Virudhunagar, TN, IN
3 CAS in Botany, University of Madras, Guindy Campus, Chennai, TN, IN

Abstract

This study illustrated that optimization of inorganic nutrients, metal ions and various cost-effective substrates yield a higher amount of solubilised protein which can be recovered from chicken feather powder by proteolytic activity of Bacillus sp. MPTK6. The test organism used in this study was isolated from feather composting soil in keratin enrichment medium at Ph 8.5. The isolate Bacillus sp. MPTK6 showed a clear zone on the keratin enriched medium. Magnesium sulphate at 0.5% yielded high protease production and solubilisation where as KH₂PO₄ and K₂HPO₄ exhibited the same effect at 0.3%. Zinc sulphate and ferrous sulphate (15 μg/ml) significantly stimulated the enzyme production and protein yield. Corn steep liquor (0.3%) and peptone (0.3%) exert a very strong stimulatory effect on protease yield as well as protein solubilisation.

Keywords

Solubilised Protein,Bacillus Sp., Magnesium Sulphate, Zinc Sulphate, Ferrous Sulphate

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