Refine your search
Collections
Co-Authors
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Kanungo, V. K.
- Study of In-vitro Micropropagation of a Medicinally Important Plant Adhatoda vasica Nees by Auxillary-bud Culture
Abstract Views :351 |
PDF Views:74
Authors
Affiliations
1 Department of Botany and Biotechnology, Laboratory of Plant Tissue Culture, Government E. Raghvendra Rao P. G. College of Science, Bilaspur - 495 006, Chhattisgarh, IN
2 Government Nagarjuna, P. G. College of science, Raipur - 492 010, Chhattisgarh, IN
1 Department of Botany and Biotechnology, Laboratory of Plant Tissue Culture, Government E. Raghvendra Rao P. G. College of Science, Bilaspur - 495 006, Chhattisgarh, IN
2 Government Nagarjuna, P. G. College of science, Raipur - 492 010, Chhattisgarh, IN
Source
Indian Journal of Innovations and Developments, Vol 1, No 4 (2012), Pagination: 199-202Abstract
The experiment was carried out at plant tissue culture laboratory, Department of Biotechnology, E.R.R.Govt Sci. Auto. P.G. College, Bilaspur, Chhattisgarh, during the period of Nov.08 2010 to May 09 2011. An efficient protocol was devised for rapid in vitro propagation through shoot bud culture of Adhatoda vasica. In present investigation the proliferating auxiliary shoot cultures were established on MS medium&Gamborg B5 medium supplemented with different concentrations of BAP, NAA, Kinetin&2, 4-D using nodal explants from the field grown mature healthy plant of Adhatoda vasica after 30 days of inoculation. In cultures raised from nodal explants of Adhatoda vasica maximum number of shoots was produced on MS medium supplemented with 1.0mg\l BAP. These explants developed greater than two shoots per nodes while in all other concentration of kn, NAA&2,4-D developed either two or less than two shoots\explants. Highest frequencies of shoot formation and maximum number of shoots per explants were obtained on MS medium supplemented with 1mg\l IBA.Keywords
Adhatoda vasica, Micropropagation, MS Medium, Nodal Explants, Shoot Bud CultureFull Text
References
- Aasim M, Khawar KM and Özcan S (2008) In Vitro micro Propagation from shoot meristems of Turkish Cowpea (Vigna unguiculata L.) cultivar Akkız. Bangladesh J. Bot., 37, 149-154.
- Bjaj M and Williams JT (1995) Healing Forests-Healing People (report on workshop on medicinal plants, 6-8 Feb., 1995, Calicut), IDRC, New Delhi, pp: 62.
- Che P, Gingerich DJ, Lall S and Howell SH (2002) Global and hormone induced gene expression changes during shoot development in Arabidopsis. Plant cell, 14, 2771-2785.
- Chopra RN (1982) Indigenous Drugs of India, Academic Publishers, Calcutta.
- Dastur JF (1985) Medicinal plants of India and Pakistan, DB Taraporevala Sons and co. pvt. Ltd., Bombay.
- Doshi JJ, Patel VK and Bhatt HV (1983) Effect of Adhatoda vasica massage in pyorrhea. Int. J. Crude Drug Res., 21, 173-176.
- Kirtikar KR and Basu BD (1994) Adhatoda Vasica Nees, India, 1899 -1902.
- Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays for tobacco tissue cultures, Physiol. Plant, 15, 431-491.
- Osman MG, Elhadi EA and Khalafalla MM (2010) Callus formation and organogenesis of tomato (Lycopersicon esculentum Mill, C.V. Omdurman) induced by thidiazuron. Afr. J. Biotechnol., 9, 4407-4413.
- Pandey R and Mishra A (2010) Antibacterial activities of crude extract of Aloe barbadensis to clinically isolated bacterial pathogens. Applied biochem. Biotechnol., 160, 1356-1361.
- Prajapati ND, Purohit SS, Sharma DD and Tarun K (2003) A Handbook of Medicinal Pants. 1st Edn., agrobiaos, Jodhpur, India, pp. 13-14.
- Prasad PJN, Chakradhar T and Pullaih T (2004) Micropropagation of Cryptolepsis buchanani roem. and schutt. Taiwania, 49, 57-65.
- Sugiyama M and Imamura K (2006) Dose time and tissue dependent effects of 5-bromo-2’-deoxyuridine on the in vitro organogenesis of Arabidopsis thaliana. Plant Cell Tissue Organ Culture, 87, 17-25.
- In Vitro Multiplication and Phyto-chemical Analysis of a Valuable Medicinal Plant Withania Somnifera L. Dunal
Abstract Views :348 |
PDF Views:68
Authors
Affiliations
1 Department of Botany and Biotechnology, Laboratory of Plant Tissue Culture, Government E. Raghvendra Rao P. G. College of Science, Bilaspur - 495006, Chhattisgarh, IN
2 Government Nagarjuna, P. G. College of science, Raipur - 492010, Chhattisgarh, IN
1 Department of Botany and Biotechnology, Laboratory of Plant Tissue Culture, Government E. Raghvendra Rao P. G. College of Science, Bilaspur - 495006, Chhattisgarh, IN
2 Government Nagarjuna, P. G. College of science, Raipur - 492010, Chhattisgarh, IN
Source
Indian Journal of Innovations and Developments, Vol 1, No 4 (2012), Pagination: 225-228Abstract
Withania somnifera, a popular Indian medicinal plant has long been used commonly in ayurvedic system of medicine. All the parts of the plants have shown remarkable importance in the field of pharmacology. The present paper gives an account of information on in-vitro micro propagation of medicinally important plant for the generation of multiple shoots and ischolar_mains of Withania somnifera and its phyto-chemical activities. Shoots were induced from the axillary buds of Withania somnifera on Murashige and Skoogs's (MS) basal media supplemented with different concentrations of BAP (0.10mg/l, 0.25mg/l, 0.50mg/l, 0.75mg/l, 1.00mg/l). Axillary bud explants showed initiation of shoots with the concentrations of 1mg/l and it is found to be most effective for multiple shoot generation within minimum time period. The leaf, stem and ischolar_main of Withania somnifera were analyzed for phyto-chemical screening. The micro propagated plants were transplanted to pots for hardening.Keywords
In-vitro Multiplication, MS Medium, Withania somnifera, Shoot Bud Culture, Benzyl Amino Purine, AxiliaryFull Text
References
- Bapat VA, Yadav SR and Dixit GB (2008) Rescue of endangered plants through biotechnological applications, Natt, Acad. Sci. Lett., 31, 201-210.
- Bhattacharya R and Bhattacharya S (2001) High frequency of in vitro propagation of Phyllanthus amarus by shoot tip culture. Indian J. Exp. Biol., 39, 1184-1187.
- Catapan E, Otuki MF and Viana AM (2000) In vitro culture of Phyllanthus caraliniensis. Plant Cell Tiss. Organ Cult. 62, 195-202.
- Dube P, Gangopadhyay M, Deewanjee S and Ali MN (2011) Establishment of a rapid multiplication protocol of Coleus forskohlii Briq and in vitro conservation by reduced growth. Indian J. Biotechnol., 10, 228-231.
- Fay MF (1992) Conservation of rare and endangered plants using in vitro methods. In Vitro Cell Dev. Biol. Plant. 28, 1-4.
- Kirtikar KR and Basu BD (2001) In: Basu LM ed. Indian Medicinal Plants. Oriental Enterprises, Allahabad, India, Vol. 8, 2445-49
- Laxshami GN and Seeni S (2001) Rapid in vitro multiplication and restoration of Celastrus paniculatus Willd. Subsps. Paniculatus (Celastraceae), a medicanal woody climber. Indian J. Exp. Biol. 39, 697-704.
- Mohamed IE, Nur El and Abdelrahman ME (2010) The antibacterial, antiviral activities and phytochemical screening of some Sudanese medicinal plants, Journal BioSci, 4, 8-16.
- Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiology Plant. 15, 473-97.
- Nagesh KS and Shanthamma C (2011) Micropropagation and antioxidant activity of Mollugo nudicaulis lam. J. Med. Plants Res. 5(6), 895-902.
- Sahoo Y, Pattnaik SK and Chand PK (1997) In vitro clonal propagation of an aromatic medicinal herb Ocimum basilicum L. (Sweet basil) by axillary shoots proliferation. In vitro cell, Devel. Biol. Plant Largo, 33, 293-296.
- Siddique I and Anis M (2007) Rapid micropropagation of Ocimum basilicum using shoot tip explants pre-cultured in thidiazuron supplemented liquid medium. Biol. Plant. 51, 787-790.
- Tripathy AK, Shukla YN and Kumar S (1996) Ashwagandha [Withania somnifera Dunal (Solanaceae)]: A status report. J. Med. & Aromat. Plant Sci., 18, 46-62.