Ramachandra Prabhu Shanmugam ¹, Saravanan Narayanasamy ¹, Rose Gregoria ¹, Madhu Magesh Kandan ¹, Moses Inbaraj Rajamanickam ¹

Affiliations
1 Department of Zoology, Madras Christian College, Tambaram, Chennai 600059, IN

Abstract

Background/Objectives: The estrogen mimics are predominantly come into the freshwater bodies and affecting the fish reproduction by increasing anthropological usages like cosmetics, pesticides, detergents, plastics and other pharmaceutical compounds. In the present study, an attempt has been made to study the effect of estrogen mimics, Bisphenol-A (BPA) and Butylhydroxyanisole (BHA) on the reproduction of Indian major carp L. rohita. Methods: Fishes were exposed to BPA and BHA at 5μM concentration for 30 days. The ovary, testis and liver was chosen to measure the Gonadosomatic index (GSI), Hepatosomatic index (HSI), histology, total protein, lipid, and acid and alkaline phosphatase enzymes activity. All the parameters showed the minimum of three to four fold reduction in the treated fishes when compare with control in its function. The estrogen mimics might play an effective role in the ovarian follicle steroid synthesis. The Present study carp oocytes were incubated with follicle growth inducing steroid precursor, pregnenolone with or without BPA and BHA to find the interference of estrogen mimics in the normal steroidogenesis. Results: The pregnenolone treated eggs showed the secretion of progesterone and 17α,20β-P production when the incubation medium analysed by TLC. The BPA and BHA treated group incubations recorded the 21-OHprogesterone. This changes might be due to the shift of enzyme from 17α- hydroxylase to 21-hydroxylase in the presence of BPA and BHA. Conclusion : From this study, it is confirm that the estrogen mimics adversely affect the process of reproduction in the Indian major carp L. rohita at various levels.

Keywords

Endocrine disruptors, Estrogen mimics, BPA, BHA, Indian carp

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