Indian Journal of Geo-Marine Sciences

Open Access Open Access  Restricted Access Subscription Access

In-Silico Analysis of Bioactive Compounds Extracted from Seaweed Amphiroa anceps on the Pathogenicity of Bacteria


Affiliations
1 Jindal Institute of Behavioural Sciences, O.P. Jindal Global University, Haryana – 131 001, India
 

Screening of biochemical compounds in marine algae is a gateway to understand the bioactive principles of antimicrobial activity and may be a significant tool in developing environment friendly herbicides and in drug discovery. In this study, the antibacterial activity of the seaweed Amphiroa anceps extracted with methanol and ethanol was determined against clinical isolates namely Vibrio cholerae 01 ogawa, Vibrio cholerae O139, Vibrio fluvialis, Vibrio parahaemolyticus, V. parahaemolyticus 81, V. parahaemolyticus O3:K6, salmonella enteritidis type 5, Enterotoxigenic E. coli, Bacillus cereus by disc diffusion method at different concentrations. Ethanolic extract showed the highest activity against V. parahaemolyticus, V. parahaemolyticus 81 and V. cholerae. The FTIR results showed the presence of major functional groups like amine, hydrogen bonded alcohols, alkanes and aromatic rings. Four major bioactive compounds were selected based on GC-MS data and used as ligands to dock in-silico against proteins of pathogenic bacteria that were used in antibiotic studies.

Keywords

Amphiroa anceps, Antibacterial Activity, Docking, Pathogens, Phytocompounds.
User
Notifications
Font Size

  • Shanmughapriya S, Manilal A, Sugathan S, Selvin J, Kiran G S, et al., Antimicrobial activity of seaweeds extracts against multi-resistant pathogens, Ann Microbiol, 58 (2008) 535-541.

  • Ryu M J, Kim A D, Kang K A, Chung H S, Kim H S, et al., The green algae Ulva fasciata Delile extract induces apoptotic cell death in human colon cancer cells, Biol Animations, 49 (2013) 74-81.

  • Park H K, Kim I H, Kim J & Nam T J, Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells, Int J Mol Med, 30 (2012) 734-738.

  • Gross H, Goeger D E, Hills P, Mooberry S L, Bellatine D L, et al., Bioactive alkaloids from the red alga Lophocladia species, J Nat Prod, 69 (2006) 640-644.

  • Schonfeld-Leber B, Marine algae as human food in Hawaii, with notes on other Polynesian islands, Ecol Food Nutr, 8 (2009) 221-228.

  • Mizuno Y, Tsukamura K, Hayakawa A & Tanuma S, Seaweed prevents breast cancer? Jpn J Cancer Res, 92 (2001) 483-487.

  • Chanda S, Dave R, Kaneria M & Nagani K, Seaweeds: A novel, untapped source of drugs from sea to combat infectious diseases, Curr Res Tech Edu Top App Microbiol Microbial Biotech, (2010) 473-480.

  • Kolanjinathan K & Saranraj P, Pharmacological efficacy of marine seaweed Gracilaria edulis, Global J Pharmac, 8 (2014) 268-

  • Ravikumar S, Ramanathan G, Gnanadesigan M, Ramu A & Vijayakumar V, In vitro antiplasmodial activity of methanolic extracts from seaweeds of Southwest Coast of India, Asian Pac J Trop Med, (2011) 862-865.

  • Nomura K, Nakamura H & Suzuki N, False fertilization in sea urchin eggs induced by diabolin, a 120K kelp protein, Biochem Biophys Res Comm, 273 (2000) 691-693.

  • Ponce N M A, Pujol C A, Damonte E B, Flores M L & Stortz C A, Fucoidans from the brown seaweed Adenocystis utricularis: extraction methods, antiviral activity and structural studies, Carbohyd Res, 338 (2003) 153-165.

  • Buckle P J, Baldo B A & Taylor K M, The anti-inflammatory activity of marine natural products–6-n-tridecylsalicylic acid, flexibilide and dendalone 3-hydroxybutyrate, Agents Actions, 10 (1980) 361-367.

  • Maruyama H, Taumachi H, Lizuka M & Nakano T, The role of NK cells in antitumor activity of dietary fucoidan from Undaria pinnatifida sporophylls (Mekabu), Planta Medica, 72 (2006) 1415-1417.

  • Nishino T & Nagumo T, Anticoagulant and antithrombin activities of over sulfated fucans, Carbohyd Res, 229 (1992) 355-362.

  • Hellio C, De La Broise D, Dufosse L, Le Gal Y & Bourgougnon N, Inhibition of marine bacteria by extracts of macroalgae: potential use for environmentally friendly antifouling paints, Mar Environ Res, 52 (2001) 231-247.

  • Lamia M, Amel M, Jacques R & Abderrahman B, Antioxidant, anti-inflammatory and antiproliferative effects of aqueous extracts of three Mediterranean brown seaweeds of the Genus Cystoseira, Iran J Pharm Res, 13 (2014) 207-220.

  • Nwosu F, Morris J, Victoria A, Derek S, Heather A R, et al., Anti-proliferative and potential anti-diabetic effects of phenolic rich extracts from edible marine algae, Food Chem, 126 (2011) 1006-1012.

  • Abirami R G & Kowsalya S, Antidiabetic activity of Ulva fasciata and its impact on carbohydrate enzymes in alloxan induced diabetic in rats, Int J Res Phytochem Pharmaco, 3 (2013) 136-141.

  • Chakraborty K, Praveen N K, Vijayan K K & Rao G S, Evaluation of phenolic contents and antioxidant activities of brown seaweeds belonging to Turbinaria spp. (Phaeophyta, Sargassaceae) collected from Gulf of Mannar, Asian Pac J Trop Biomed, 3 (2013) 8-16.

  • Smit A J, Medicinal and pharmaceutical uses of seaweed natural products: A review, J Appl Phycol, 16 (2004) 245-262.

  • Ana M, Helena V, Helena G & Susana S, Marketed marine natural products in the pharmaceutical and cosmeceutical industries: Tips for success, Mar Drugs, 12 (2014) 1066-1101.

  • Maschek J A & Baker B J, The Chemistry of Algal Secondary Metabolism, In: Algal Chemical Ecology, edited by C D Amsler, (Springer), 2008, pp. 313.

  • Lamouroux J V F, Extrait d’um mémoire sur la classification des Polypiers Coralligenes non entierement pierreux. Nouvaux Bulletin des Sciences, Publiqué par la Societé Philomatique de., 3 (1812) 181-188.

  • Antonisamy J & Sankara Raj E D, UV–VIS and HPLC studies on Amphiroa anceps (Lamarck) Decaisne, Arabian J Chem, 9 (1) (2016) S907-S913.

  • Grosdidier S & Fernandez-Recio J, Protein-protein docking and hot-spot prediction for drug discovery, Curr Pharm Des, 18 (2012) 4607-4618.

  • Avram S, Funar-Timofei S, Borota A, Chennamaneni S R, Manchala A K, et al., Quantitative estimation of pesticide-likeness for agrochemical discovery, J Cheminfo, 6 (42) (2014) pp. 11.

  • Bauer A W, Kirby W M, Sherris J C & Turck M, Antibiotic susceptibility testing by a standardized single disk method, Am J Clin Pathol, 45 (1966) 493-496.

  • Kim M M, Mendis E & Kim S K, Laurencia okamurai extract containing laurinterol induces apoptosis in melanoma cell, J Med Food, 11 (2008) 260-266.

  • El-Gamal A A, Biological importance of marine algae, Saudi Pharm J, 18 (2010) 1-25.

  • Haefner B, Drugs from the deep: marine natural products as drug candidates, Drug Discov Today, 8 (2003) 536-544.

  • Silva P C, Basson P W & Moe R L, Catalogue of the benthic marine algae of the Indian Ocean, University of California Publications in Botany, 79 (1996) 1-1259.

  • Lubobi S F, Matunda C, Kumar V & Omboki B, Isolation of Bioactive Secondary Metabolites from Seaweeds Amphiroa anceps against Chicken Meat Associated Pathogens, J Antimicro, 2 (2016) 113.

  • Taskin E, Ozturk M & Taskin E K, Antibacterial activities of some marine algae from the Aegean Sea (Turkey), Afr J Biotechnol, 6 (2007) 2746-2751.

  • Elsie B H & Dhana Rajan M S, Evaluation of antimicrobial activity and phytochemical screening of Gelidium acerosa, J Pharm Sci & Res, 2 (2010) 704-707.

  • Kolanjinathan K & Stella D, Pharmacological Effect of Gracilaria corticata Solvent extracts against human pathogenic bacteria and fungi, Int J Pharm, 6 (2011) 1722-1728.

  • Vijayabaskar P & Shiyamala V, Antibacterial activities of brown marine algae (Sargassum wightii and Turbinaria ornata) from the Gulf of Mannar Biosphere Reserve, Adv Biol Res, 5 (2011) 99-102.

  • Lincoln R A, Strupinski K & Walker J M, Bioactive compounds from algae, Life Chem Rep, 8 (1991) 97-183.

  • Alagic S, Stancic I, Palic R, Stojanovic G & Lepojevic Z, Chemical composition of the supercritical CO2 extracts of the Yaka, Prilep and Otlja tobaccos, J Essential Oil Res, 18 (2006) 185-188.

  • Cowan M M, Plant Products as Antimicrobial Agents, Clin Microbiol Rev, 12 (1999) 564-582.

  • Pfefferle C, Kempter C, Metzger J W & Fiedler H P, E-4-oxonon-2-enoic acid, an antibiotically active fatty acid produced by Streptomyces olivaceus Tu 4018, J Antibiot, 49 (1996) 826-828.

  • Zheng C J, Yoo J S, Lee T G, Cho H Y, et al., Fatty acid synthesis is a target for antibacterial activity of unsaturated fatty acids, FEBS Lett, 579 (2005) 5157-5162.

  • Rathinavelan T, Tang C, De Guzman R N J, Characterization of the interaction between the Salmonella type III secretion system tip protein SipD and the needle protein PrgI by paramagnetic relaxation enhancement, Biol Chem, 286 (2010) 4922-4930.


Abstract Views: 86

PDF Views: 54




  • In-Silico Analysis of Bioactive Compounds Extracted from Seaweed Amphiroa anceps on the Pathogenicity of Bacteria

Abstract Views: 86  |  PDF Views: 54

Authors

D. Madathil
Jindal Institute of Behavioural Sciences, O.P. Jindal Global University, Haryana – 131 001, India
R. Chidambaram
Jindal Institute of Behavioural Sciences, O.P. Jindal Global University, Haryana – 131 001, India

Abstract


Screening of biochemical compounds in marine algae is a gateway to understand the bioactive principles of antimicrobial activity and may be a significant tool in developing environment friendly herbicides and in drug discovery. In this study, the antibacterial activity of the seaweed Amphiroa anceps extracted with methanol and ethanol was determined against clinical isolates namely Vibrio cholerae 01 ogawa, Vibrio cholerae O139, Vibrio fluvialis, Vibrio parahaemolyticus, V. parahaemolyticus 81, V. parahaemolyticus O3:K6, salmonella enteritidis type 5, Enterotoxigenic E. coli, Bacillus cereus by disc diffusion method at different concentrations. Ethanolic extract showed the highest activity against V. parahaemolyticus, V. parahaemolyticus 81 and V. cholerae. The FTIR results showed the presence of major functional groups like amine, hydrogen bonded alcohols, alkanes and aromatic rings. Four major bioactive compounds were selected based on GC-MS data and used as ligands to dock in-silico against proteins of pathogenic bacteria that were used in antibiotic studies.

Keywords


Amphiroa anceps, Antibacterial Activity, Docking, Pathogens, Phytocompounds.

References