Indian Journal of Forensic Medicine & Toxicology

Mariam T. Abbdul-Kaliq ¹, Khetam H. Rasool ¹, Rajwa Hasen Essa ¹

Affiliations
1 Department of Biology, College of Science, Mustansiriyah University, Baghdad, IQ

Abstract

Lactococcus lactis is a Gram-positive bacterium used widely in the production of cheese and buttermilk, but has moreover become well-known as the first genetically modified organism to be used alive for the treatment of human diseases. This study was designed to display the efficacy of lactococcus lactis, which was isolated from raw cow’s milk isolated by using the liquid MRS agar, culture, and microscopically was tested moreover, chemically was also tested to check the presence of (catalase, oxidase, protease production, gelateinase, clot formation). The isolates were confirmed using molecular diagnosis. The Bacterial isolates were developed on the M17 liquid medium at 30 °C for 24 hrs to obtain the raw bacteriocin after separation by centrifuge. The toxicity and bacterial susceptibility was tested as well the effect of Bacteriocin on E. coli, P. aerugionsa, staph. areus. The results showed a clear inhibitory effect of bacteriocin on pathogenic bacteria. The toxic effect of crude bacteriocin was investigated and its effect on cancer cell lines of breast cancer cells (MCF-7) lymphoid cell line (CCL-119) using different concentrations of the extract. The toxic effect was also examined on normal liver cell lines (WRL) with a 72 hrs incubation period. The results show a clear and high-significant effect of crude bacteriocin on cancer cells. It was also observed that the severity of toxicity increased with the increases of concentration with no effect on normal cell lines.

Keywords

Lactococcus lactis, Cow Milk, Bacteriocin, Cancer Cell Lines.

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Qusay Abdul-Wahab Khalaf ¹, Rajwa Hasen Essa ¹, Khetam Habeeb Rasool ¹

Affiliations
1 Department of Biology, College of Science, Mustansiriyah University, Baghdad, IQ

Abstract

Type 2 diabetes mellitus (T2DM) is one of the metabolic diseases characterized by hyperglycemia and includes individuals who have relatively insulin deficiency and insulin resistance. Helicobacter pylori (H. pylori) is a spiral-shaped bacterium, infects 50% of the world’s people, and inhabits in the human gastric epithelium, causing many diseases. It had been suggested that H. pylori infection is more frequent among T2DM patients. Aim of this study is to evaluate the levels of interferon gamma (IFN-γ) in Iraqi patients with type 2 diabetes mellitus infected by H. pylori bacteria. Anti-H.pylori IgG and IFN-γ concentrations were measured by enzyme-linked immunosorbent assay (ELISA). The study includes 140 patients, divided into 3 groups, which are a group of patients infected by T2DM and H.pylori (T2DMHp+ve), a group of patients infected by T2DM but not by H. pylori group (T2DMHp-ve), and the third group includes patients infected with H. pylori only (Hp), in addition to apparently healthy control group (AHC). Our study revealed significantly decreasing in serum IFN-γ in both T2DMHp+ve group (P<0.05) and T2DMHp-ve group (P<0.01), compared with an apparently healthy control group(AHC). Also, this study shows decreased IFN-γ level in patients with Hp group compared with healthy control but without significant difference. Also, the concentration of IFN-γ in T2DMHp+ve is slightly more than that in T2DMHp-ve group.

Keywords

T2DM, Anti-H. Pylori IgG, IFN-γ

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