Indian Journal of Bioinformatics and Biotechnology

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Molecular Cloning and Analysis of Glycoprotein Genes C, D and I of an Isolated Strain of Marek's Disease Virus Type-3


Affiliations
1 Department of Biotechnology, University of Kerala,Thiruvananthapuram, Kerala, India
 

Marek's disease is the most commonly occurring neoplasm of any animal population and until recently it has caused extensive economic loss globally. Marek's virus, an actively evolving virus is the causative agent. Now with the virus's genetic code in hand we could easily characterise the immunogenic proteins of the closest relative of the virus, the Herpes virus of Turkey's.The important glycoprotein genes gC, gD,and gI of an isolated strain of Herpes virus of Turkey's were amplified, cloned in pGEM T-Easy vector and sequenced as an initial step in developing a recombinant vaccine against Marek's Disease. The recombinant genes were analysed and sequence data confirmed that the isolated strain was HVT (FC126), a vaccine strain.

Keywords

Marek's Disease, Oncogenic, Glycoproteins, Herpesvirus, Poultry
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  • Molecular Cloning and Analysis of Glycoprotein Genes C, D and I of an Isolated Strain of Marek's Disease Virus Type-3

Abstract Views: 510  |  PDF Views: 331

Authors

Smitha Sudhakar
Department of Biotechnology, University of Kerala,Thiruvananthapuram, Kerala, India
A. Jayakumaran Nair
Department of Biotechnology, University of Kerala,Thiruvananthapuram, Kerala, India

Abstract


Marek's disease is the most commonly occurring neoplasm of any animal population and until recently it has caused extensive economic loss globally. Marek's virus, an actively evolving virus is the causative agent. Now with the virus's genetic code in hand we could easily characterise the immunogenic proteins of the closest relative of the virus, the Herpes virus of Turkey's.The important glycoprotein genes gC, gD,and gI of an isolated strain of Herpes virus of Turkey's were amplified, cloned in pGEM T-Easy vector and sequenced as an initial step in developing a recombinant vaccine against Marek's Disease. The recombinant genes were analysed and sequence data confirmed that the isolated strain was HVT (FC126), a vaccine strain.

Keywords


Marek's Disease, Oncogenic, Glycoproteins, Herpesvirus, Poultry

References