The Asian Journal of Horticulture

S. Malthi ¹, V. P. Singh ¹, S. S. Saraswati ¹, H. T. Sakhubai ¹, P. M. Preeti ¹

Affiliations
1 College of Horticulture, University of Horticultural Sciences, Bagalkot (Karnataka), IN

Abstract

An experiment on tissue culture studies in long pepper (Piper longum L.) was carried out in the year 1999-2001, to develop suitable protocol for plant multiplication technique at Green Earth Biotechnologies Ltd., Jigani Industrial Area, Bengaluru in collaboration with UAS, GKVK, Bengaluru. The concentration of 0.1 per cent mercuric chloride (HgCl₂) for 60 seconds to achieve disinfection of leaf segment explants was found effective, giving maximum survival (10.02 %) and minimum contamination (0.70 %). MS media containing BAP (1.5 mg/lit.) + 2,4-D (1.0 mg/lit.) from leaf explants gave higher (9.72 %) response for calli induction, 1.54 mg amount of callus with green colour and very good score of callus. BAP 3.0 mg/lit. was ideal for the better proliferation and regeneration of shoot (40 %).The Highest number of multiple shoots (2.00), Length of shoot (1.04 cm) and number of leaves per shoot (3.00), was produced using BAP (1.0 mg/lit.) + NAA(0.1 mg/lit.). Best results for shoot were observed with the MS media combination containing BAP at 1.5 mg/lit., Kinetin at 1.0 mg/lit. and IAA at 0.5 mg/lit., gave the maximum number of buds per calli (36.00) and number of shoots (5.20) and length of shoots (1.62 cm). The in vitro ischolar_maining was achieved with the application of NAA at 1.0 mg/l 70 per cent ischolar_maining, number of primary ischolar_mains (2.40) length of primary ischolar_main (2.00) and number of secondary ischolar_mains (2.00).

Keywords

Long Pepper, In Vitro, Micropropagation, Tissue Culture, MS Media.

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B. S. Harish ¹, V. P. Singh ², Y. C. Vishwanath ², H. T. Sakhubai ³, Ganesh N. Bhat ³

Affiliations
1 College of Horticulture, Mysore (Karnataka), IN
2 College of Horticulture, Bagalkot (Karnataka), IN
3 University of Horticultural Sciences, Bagalkot, Dyanagiri (Karnataka), IN

Abstract

Scented geranium (Pelargonium spp.: Geraniaceae) is an important, high value aromatic crop of South African origin. Due to high demand and price for the oil, an excellent potential exists for increasing cultivated area in India. An attempt was made to evaluate and characterize the available accessions of scented geranium based on their physico-chemical properties, chemical composition and odour assessment of the oil. There were seven treatments and four replications. All the accessions possessed light yellow coloured oil except PG-10, which possessed light green colour. The oil from PG-10 showed maximum acid value (3.02). The oil from PG-8 recorded the highest ester value (58.737). The oil form KB and CIMAP possessed maximum citronellol and geraniol content while, linalool was found to be maximum in case of PG-11. PG-10 contained maximum isomanthone (8.50%). The oil from PG-1 recorded the highest citronellyl formate content (15.83%). The essential oil was distilled during August from seven accessions upon GC analysis. The concentrations of isomenthone, citronellyl formate and citronellol were maximum in case of PF-10 (7.74, 23.18 and 37.07%, respectively). The major alcohols i.e., citronellol, geraniol, linalool and nerol were maximum in case of the oils of KB, CIMAP, PG-8 and PG-1 during May. Whereas, during August, PG-10 recorded the maximum nerol and cironellol whereas, PG-12 registered the highest concentration of geraniol and linalool. The rosy odour of different accessions is attributed to the presence of higher levels of geraniol, which is evident in the present case with the accession PG-12, during August. Hence, all the accessions differed significantly in all the parameters.

Keywords

Geranium, Accessions, Physico-Chemical Properties, Chemical Composition, Odour Assessment.

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