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Badirzadeh, Alireza
- Immunization of Sheep Against Echinococcus granulosus With Protoscolex Tegumental Surface Antigens
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Materials and Methods: Ten lambs which were infected with CE (positive control), 10 negative control, and 10 test groups of sheep were included in the study. 300 μg emulsion of purified-PSTSA was injected intramuscularly in a two-step immunization on the first and 30 days. Sera were collected immediately before immunization and 6 times with 10-day intervals until 60 days post immunization. Thereafter, the sera were tested for antibodies by indirect hemagglutination test in microtiter plate.
Results: After two immunizations, all the infected animals in test group showed substantial increases in antibody titer. Statistical analysis showed a significant difference between the titer obtained in the test and negative control groups in both phases of immunization (p<0.05).
Conclusion: The results showed that the PSTSA is a promising immunogenic compound for immunization of sheep against CE.
Authors
Manouchehr Valizadeh
1,
Behzad Haghpanah
2,
Alireza Badirzadeh
1,
Elham Roointan
1,
Shirzad Fallahi
3,
Saber Raeghi
4
Affiliations
1 Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR
2 Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR
3 Department of Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, IR
4 Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, IR
1 Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR
2 Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR
3 Department of Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, IR
4 Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, IR
Source
Veterinary World, Vol 10, No 8 (2017), Pagination: 854-858Abstract
Aim: Cystic echinococcosis (CE) has potential economic effects to both animal products and human health. A vaccine to protect livestock against CE can be effective in reducing economic costs and increasing the livestock products. Protoscolex tegumental surface antigens (PSTSA) used to induce the production of specific antibodies against Echinococcus granulosus in sheep. The tegumental antigens were extracted from viable protoscolices by solubilization in sterile phosphate-buffered saline containing decanoyl-N-methylglucamine.Materials and Methods: Ten lambs which were infected with CE (positive control), 10 negative control, and 10 test groups of sheep were included in the study. 300 μg emulsion of purified-PSTSA was injected intramuscularly in a two-step immunization on the first and 30 days. Sera were collected immediately before immunization and 6 times with 10-day intervals until 60 days post immunization. Thereafter, the sera were tested for antibodies by indirect hemagglutination test in microtiter plate.
Results: After two immunizations, all the infected animals in test group showed substantial increases in antibody titer. Statistical analysis showed a significant difference between the titer obtained in the test and negative control groups in both phases of immunization (p<0.05).
Conclusion: The results showed that the PSTSA is a promising immunogenic compound for immunization of sheep against CE.