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Toxoplasma gondii Type I, Predominant Genotype Isolated from Sheep in South of Iran


Affiliations
1 Department of Parasitology and Mycology, Jahrom University of Medical Sciences, Jahrom, Iran, Islamic Republic of
2 Department of Parasitic Disease, Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran, Islamic Republic of
3 Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran, Islamic Republic of
4 Department of Biology, Islamic Azad University, Jahrom Branch, Jahrom, Iran, Islamic Republic of
 

Aim: This study was performed to determine the genetic diversity of Toxoplasma gondii in sheep using nested-polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in Southern Iran.
Materials and Methods: The tissue samples of diaphragm and heart from 125 sheep were collected from the main slaughterhouses of Jahrom district in South of Fars province, Iran, between Aprils and June 2013. The DNA were extracted and analyzed by nested-PCR using specific primers for SAG2 and GRA6 loci. RFLP was used to classify strains into one of the three major lineages of T. gondii.
Results: T. gondii Type I was predominant in this area. The data obtained from both loci demonstrated that the frequency of each genotype was 72% Type I, 2.4% Type III, 7.2% mixed Type I and II, 16.8% mixed Type I and III, 0.8% mixed Type II and III, and 0.8% mixed Type I, II and III.
Conclusions: Although the previously published data indicated that Type II is the predominant T. gondii genotype in sheep in the other parts of the world, this study showed that genotype I is the dominant genotype of T. gondii in the southern Iran; however, other genotypes were detected. High diversity of T. gondii genotypes including mix genotypes in lambs is of importance for the public health. These studies depict a new mapping of T. gondii genotypes pattern which could be very helpful in toxoplasmosis control and prevention.

Keywords

Genotyping, GRA6 Gene, Iran, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, SAG2 Gene, Sheep, Toxoplasma gondii.
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  • Toxoplasma gondii Type I, Predominant Genotype Isolated from Sheep in South of Iran

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Authors

Belal Armand
Department of Parasitology and Mycology, Jahrom University of Medical Sciences, Jahrom, Iran, Islamic Republic of
Kavous Solhjoo
Department of Parasitology and Mycology, Jahrom University of Medical Sciences, Jahrom, Iran, Islamic Republic of
Manoochehr Shabani Kordshooli
Department of Parasitic Disease, Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran, Islamic Republic of
Mohammad Hasan Davami
Department of Parasitology and Mycology, Jahrom University of Medical Sciences, Jahrom, Iran, Islamic Republic of
Morteza Pourahmad
Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran, Islamic Republic of
Vahideh Orfaee
Department of Biology, Islamic Azad University, Jahrom Branch, Jahrom, Iran, Islamic Republic of

Abstract


Aim: This study was performed to determine the genetic diversity of Toxoplasma gondii in sheep using nested-polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in Southern Iran.
Materials and Methods: The tissue samples of diaphragm and heart from 125 sheep were collected from the main slaughterhouses of Jahrom district in South of Fars province, Iran, between Aprils and June 2013. The DNA were extracted and analyzed by nested-PCR using specific primers for SAG2 and GRA6 loci. RFLP was used to classify strains into one of the three major lineages of T. gondii.
Results: T. gondii Type I was predominant in this area. The data obtained from both loci demonstrated that the frequency of each genotype was 72% Type I, 2.4% Type III, 7.2% mixed Type I and II, 16.8% mixed Type I and III, 0.8% mixed Type II and III, and 0.8% mixed Type I, II and III.
Conclusions: Although the previously published data indicated that Type II is the predominant T. gondii genotype in sheep in the other parts of the world, this study showed that genotype I is the dominant genotype of T. gondii in the southern Iran; however, other genotypes were detected. High diversity of T. gondii genotypes including mix genotypes in lambs is of importance for the public health. These studies depict a new mapping of T. gondii genotypes pattern which could be very helpful in toxoplasmosis control and prevention.

Keywords


Genotyping, GRA6 Gene, Iran, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, SAG2 Gene, Sheep, Toxoplasma gondii.