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Detection of Toxoplasma gondii in Cat’s Internal Organs by Immunohistochemistry Methods Labeled With-[Strept] Avidin-Biotin


Affiliations
1 Parasitology Laboratory, Syiah Kuala University, Banda Aceh, Indonesia
2 Department of Parasitology, Gadjah Mada University, Yogyakarta, Indonesia
3 Veterinary Public Health of Laboratory, Diseases Investigation Center, Wates, Yogyakarta, Indonesia
4 Department of Clinic, Gadjah Mada University, Yogyakarta, Indonesia
5 Department of Pathology, FKH UGM, Sekip Unit II Yogyakarta 55281, Yogyakarta, Indonesia
6 Pathology Laboratory, Syiah Kuala University Banda Aceh, Indonesia
 

Aim: The aims of the study are to detect the presence of Toxoplasma gondii antigen and to determine its distribution location in several organs of domestic cat using immunohistochemistry (IHC) method with Labeled-[Strept] Avidin-Biotin (LAB-SA).
Material and Methods: Four domestic cats aged 1-2 years were used as sample in this research. The sample divided into two groups with two cats each. Cats in Group I were positive Toxoplasma based on serologically screening test, while cats in Group II were orally infected with 1×106 Toxoplasma oocyst. All samples then necropsied, and the organs including brain, liver, kidney, duodenum, jejunum, ileum, lungs, and spleen were collected for IHC method with LAB-SA.
Result: The result showed that Toxoplasma antigens were detected in ileum of both serologically positive domestic cat and the experimentally infected cats. Toxoplasma was also observed in kidney of serologically positive domestic cat. In the serologically positive domestic cat, necrotic lesions were found on ileum, kidney, and liver, whereas in experimentally infected cat, the lesion was only found on ileum.
Conclusion: The presence of Toxoplasma antigen is successfully detected in several organs of domestic cat using IHC method with the LAB-SA.

Keywords

Cat, Immunohistochemistry, Labeled-[Strept] Avidin-Biotin, Toxoplasma gondii.
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  • Detection of Toxoplasma gondii in Cat’s Internal Organs by Immunohistochemistry Methods Labeled With-[Strept] Avidin-Biotin

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Authors

Muhammad Hanafiah
Parasitology Laboratory, Syiah Kuala University, Banda Aceh, Indonesia
Raden Wisnu Nurcahyo
Department of Parasitology, Gadjah Mada University, Yogyakarta, Indonesia
Rika Yuniar Siregar
Veterinary Public Health of Laboratory, Diseases Investigation Center, Wates, Yogyakarta, Indonesia
Joko Prastowo
Department of Parasitology, Gadjah Mada University, Yogyakarta, Indonesia
Sri Hartati
Department of Clinic, Gadjah Mada University, Yogyakarta, Indonesia
Bambang Sutrisno
Department of Pathology, FKH UGM, Sekip Unit II Yogyakarta 55281, Yogyakarta, Indonesia
Dwinna Aliza
Pathology Laboratory, Syiah Kuala University Banda Aceh, Indonesia

Abstract


Aim: The aims of the study are to detect the presence of Toxoplasma gondii antigen and to determine its distribution location in several organs of domestic cat using immunohistochemistry (IHC) method with Labeled-[Strept] Avidin-Biotin (LAB-SA).
Material and Methods: Four domestic cats aged 1-2 years were used as sample in this research. The sample divided into two groups with two cats each. Cats in Group I were positive Toxoplasma based on serologically screening test, while cats in Group II were orally infected with 1×106 Toxoplasma oocyst. All samples then necropsied, and the organs including brain, liver, kidney, duodenum, jejunum, ileum, lungs, and spleen were collected for IHC method with LAB-SA.
Result: The result showed that Toxoplasma antigens were detected in ileum of both serologically positive domestic cat and the experimentally infected cats. Toxoplasma was also observed in kidney of serologically positive domestic cat. In the serologically positive domestic cat, necrotic lesions were found on ileum, kidney, and liver, whereas in experimentally infected cat, the lesion was only found on ileum.
Conclusion: The presence of Toxoplasma antigen is successfully detected in several organs of domestic cat using IHC method with the LAB-SA.

Keywords


Cat, Immunohistochemistry, Labeled-[Strept] Avidin-Biotin, Toxoplasma gondii.