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Effects of Hyperbaric Therapy on Liver Morphofunctional of Rabbits (Oryctolagus cuniculus) After Hind Limb Ischemia-Reperfusion Injury


Affiliations
1 Department of Veterinary Clinic, Universitas Airlangga, Jl. Mulyorejo, Kampus C, Surabaya-60115, Indonesia
2 Department of Surgery, Division of Thoracic, Cardiac, and Vascular Surgery, Dr. Soetomo General Hospital, Surabaya-60115, Indonesia
 

Aim: The objective of this research was to study and to prove the effectiveness of hyperbaric oxygen therapy (HBOT) starting time on liver morphofunctional changes after ischemia-reperfusion in the hind limb of rabbits.
Materials and Methods: This research used a complete randomized design with 4 groups and 6 repetitions on each. After 6 h artery femoral is ligation, reperfusion was performed for 100 min (G1), HBOT for 90 min after 10 min reperfusion (G2), 10 min reperfusion (G3), and HBOT 90 min after 60 min reperfusion (G4). Then, all of the rabbits were sacrificed. The liver and blood were taken for histopathological changes examination as well as for measuring the level of aspartate aminotransferase (AST) and alanine aminotransferase (ALT). The statistical test using Kruskal-Wallis and Mann-Whitney showed that the score of degeneration, necrosis, and portal inflammation in groups without HBOT (G1 and G3) were not significantly different, as well as in group with HBOT (G2 and G4) (p>0.05). However, the scores of histopathological changes in G1 and G3 were significantly different from those in G2 and G4 (p<0.05). The levels of AST and ALT in the groups without hyperbaric therapy (G1 and G3) were not significantly different from those in the groups treated with hyperbaric therapy (G2 and G4) (p>0.05).
Results: Hind limb ischemia injury reperfusion can trigger damage for liver morphology, but not lead to liver dysfunction. Reperfusion can trigger increased activity of neutrophils, while neutrophil infiltration in the organ will lead to dysfunction. HBOT can inhibit the activity of neutrophils and the dysfunction of organs caused by ischemic reperfusion.
Conclusion: HBOT for 90 min, both 10 and 60 min after the reperfusion, can protect hepatocytes from damage.

Keywords

Hyperbaric Oxygen Therapy, Ischemia, Liver, Morphofunctional, Reperfusion.
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  • Effects of Hyperbaric Therapy on Liver Morphofunctional of Rabbits (Oryctolagus cuniculus) After Hind Limb Ischemia-Reperfusion Injury

Abstract Views: 168  |  PDF Views: 0

Authors

Bambang Sektiari Lukiswanto
Department of Veterinary Clinic, Universitas Airlangga, Jl. Mulyorejo, Kampus C, Surabaya-60115, Indonesia
Wiwik Misaco Yuniarti
Department of Veterinary Clinic, Universitas Airlangga, Jl. Mulyorejo, Kampus C, Surabaya-60115, Indonesia
Y. Yosis Motulo
Department of Surgery, Division of Thoracic, Cardiac, and Vascular Surgery, Dr. Soetomo General Hospital, Surabaya-60115, Indonesia

Abstract


Aim: The objective of this research was to study and to prove the effectiveness of hyperbaric oxygen therapy (HBOT) starting time on liver morphofunctional changes after ischemia-reperfusion in the hind limb of rabbits.
Materials and Methods: This research used a complete randomized design with 4 groups and 6 repetitions on each. After 6 h artery femoral is ligation, reperfusion was performed for 100 min (G1), HBOT for 90 min after 10 min reperfusion (G2), 10 min reperfusion (G3), and HBOT 90 min after 60 min reperfusion (G4). Then, all of the rabbits were sacrificed. The liver and blood were taken for histopathological changes examination as well as for measuring the level of aspartate aminotransferase (AST) and alanine aminotransferase (ALT). The statistical test using Kruskal-Wallis and Mann-Whitney showed that the score of degeneration, necrosis, and portal inflammation in groups without HBOT (G1 and G3) were not significantly different, as well as in group with HBOT (G2 and G4) (p>0.05). However, the scores of histopathological changes in G1 and G3 were significantly different from those in G2 and G4 (p<0.05). The levels of AST and ALT in the groups without hyperbaric therapy (G1 and G3) were not significantly different from those in the groups treated with hyperbaric therapy (G2 and G4) (p>0.05).
Results: Hind limb ischemia injury reperfusion can trigger damage for liver morphology, but not lead to liver dysfunction. Reperfusion can trigger increased activity of neutrophils, while neutrophil infiltration in the organ will lead to dysfunction. HBOT can inhibit the activity of neutrophils and the dysfunction of organs caused by ischemic reperfusion.
Conclusion: HBOT for 90 min, both 10 and 60 min after the reperfusion, can protect hepatocytes from damage.

Keywords


Hyperbaric Oxygen Therapy, Ischemia, Liver, Morphofunctional, Reperfusion.