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Immunoglobulin Subclass in Experimental Murine Toxocara cati Infection


Affiliations
1 Department of Parasitology, Universitas Airlangga, Surabaya, East Java, Indonesia
 

Aim: The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human.
Materials and Methods: The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs of Toxocara cati. The optical density values of anti-T. cati mice serum IgG subclasses were analyzed by applying ANOVA factorial.
Results: The specific IgG subclass in mice infected with T. cati mice was found to be IgG2β.
Conclusion: Subclass of IgG, especially IgG2β, can provide leads about the use of the monoclonal antibody in conjugate making an effort for the indirect ELISA diagnostic kit.

Keywords

Diagnostic Kit, Enzyme-Linked Immunosorbent Assay, Human, Immunoglobulin G Subclass, Monoclonal Antibody, Toxocariasis, Toxocara cati.
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  • Immunoglobulin Subclass in Experimental Murine Toxocara cati Infection

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Authors

Kusnoto
Department of Parasitology, Universitas Airlangga, Surabaya, East Java, Indonesia
Setiawan Koesdarto
Department of Parasitology, Universitas Airlangga, Surabaya, East Java, Indonesia
Sri Mumpuni
Department of Parasitology, Universitas Airlangga, Surabaya, East Java, Indonesia
R. Heru Prasetyo
Department of Parasitology, Universitas Airlangga, Surabaya, East Java, Indonesia

Abstract


Aim: The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human.
Materials and Methods: The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs of Toxocara cati. The optical density values of anti-T. cati mice serum IgG subclasses were analyzed by applying ANOVA factorial.
Results: The specific IgG subclass in mice infected with T. cati mice was found to be IgG2β.
Conclusion: Subclass of IgG, especially IgG2β, can provide leads about the use of the monoclonal antibody in conjugate making an effort for the indirect ELISA diagnostic kit.

Keywords


Diagnostic Kit, Enzyme-Linked Immunosorbent Assay, Human, Immunoglobulin G Subclass, Monoclonal Antibody, Toxocariasis, Toxocara cati.