Open Access Open Access  Restricted Access Subscription Access

Production of Aldosterone in Cardiac Tissues of Healthy Dogs and with Dilated Myocardiopathy


Affiliations
1 Meritorious Autonomous University of Puebla, Tecamachalco Puebla, Mexico
 

Background and Aim: Aldosterone is a hormone, belonging to the group of mineralocorticoids, mainly synthesized in the adrenal cortex, basically its function is to regulate blood pressure and sodium‑potassium levels in the body; high levels of this hormone have harmful effects in the organism and mainly in the heart in chronic form. Dilated cardiomyopathy is a progressive disease of heart muscle that is characterized by ventricular chamber enlargement and contractile dysfunction, is one of the most common cardiac conditions in dogs of medium and large breeds. The aim of the study was to determine and quantify if a dog’s cardiac cells possess the capacity to synthesize aldosterone, as well as, the differences that appear between a healthy heart and with dilated myocardiopathy (DMC).
Materials and Methods: Cardiac tissues were used from six healthy dogs and six with DMC. Enzyme‑linked immunosorbent assay was performed to determine if the dog’s heart cells synthesized this mineralocorticoid in a similar way to rat, rabbit, and human tissues, as well as quantitative differences between the healthy heart and DMC.
Results: In healthy dog hearts, aldosterone values were 62.5 pG for both the atria and right ventricle and 125 pG for the left ventricle. As for dog hearts’ with DMC, results were 125 pG in all four cavities.
Conclusion: Both the healthy and DMC dog hearts produce aldosterone in all four cavities, observing that production increases in the atria and right ventricle of those hearts with DMC, as an intrinsic mechanism of cardiac remodeling.

Keywords

Cardiac Insufficiency, Dog Heart, Enzyme‑Linked Immunosorbent Assay, Mineralocorticoids.
User
Notifications
Font Size

Abstract Views: 178

PDF Views: 0




  • Production of Aldosterone in Cardiac Tissues of Healthy Dogs and with Dilated Myocardiopathy

Abstract Views: 178  |  PDF Views: 0

Authors

Alejandro Reynoso‑Palomar
Meritorious Autonomous University of Puebla, Tecamachalco Puebla, Mexico
Georgina Mena‑Aguilar
Meritorious Autonomous University of Puebla, Tecamachalco Puebla, Mexico
Marisol Cruz‑Garcia
Meritorious Autonomous University of Puebla, Tecamachalco Puebla, Mexico
Cesar Pastelin‑Rojas
Meritorious Autonomous University of Puebla, Tecamachalco Puebla, Mexico
Abel Villa‑Mancera
Meritorious Autonomous University of Puebla, Tecamachalco Puebla, Mexico

Abstract


Background and Aim: Aldosterone is a hormone, belonging to the group of mineralocorticoids, mainly synthesized in the adrenal cortex, basically its function is to regulate blood pressure and sodium‑potassium levels in the body; high levels of this hormone have harmful effects in the organism and mainly in the heart in chronic form. Dilated cardiomyopathy is a progressive disease of heart muscle that is characterized by ventricular chamber enlargement and contractile dysfunction, is one of the most common cardiac conditions in dogs of medium and large breeds. The aim of the study was to determine and quantify if a dog’s cardiac cells possess the capacity to synthesize aldosterone, as well as, the differences that appear between a healthy heart and with dilated myocardiopathy (DMC).
Materials and Methods: Cardiac tissues were used from six healthy dogs and six with DMC. Enzyme‑linked immunosorbent assay was performed to determine if the dog’s heart cells synthesized this mineralocorticoid in a similar way to rat, rabbit, and human tissues, as well as quantitative differences between the healthy heart and DMC.
Results: In healthy dog hearts, aldosterone values were 62.5 pG for both the atria and right ventricle and 125 pG for the left ventricle. As for dog hearts’ with DMC, results were 125 pG in all four cavities.
Conclusion: Both the healthy and DMC dog hearts produce aldosterone in all four cavities, observing that production increases in the atria and right ventricle of those hearts with DMC, as an intrinsic mechanism of cardiac remodeling.

Keywords


Cardiac Insufficiency, Dog Heart, Enzyme‑Linked Immunosorbent Assay, Mineralocorticoids.