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Genotyping of Virulent Escherichia coli Obtained from Poultry and Poultry Farm Workers Using Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction


Affiliations
1 Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Sri Venkateswara Veterinary University, Gannavaram, Andhra Pradesh, India
2 Department of Veterinary Microbiology, College of Veterinary Science, Tirupati, Andhra Pradesh, India
3 Department of Animal Genetics and Breeding, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India
 

Aim: The aim of this study was to characterize virulent Escherichia coli isolated from different poultry species and poultry farm workers using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) genotyping.
Materials and Methods: Fecal swabs from different poultry species (n=150) and poultry farm workers (n=15) were analyzed for E. coli and screened for virulence genes (stx1, stx2, eaeA, and hlyA) by multiplex PCR. Virulent E. coli was serotyped based on their “O” antigen and then genotyped using ERIC-PCR.
Results: A total of 134 E. coli isolates (122/150 from poultry and 12/15 from farm workers) were recovered. Virulence genes were detected in a total of 12 isolates. Serological typing of the 12 virulent E. coli revealed nine different serotypes (O2, O49, O60, O63, O83, O101, O120, UT, and Rough). ERIC-PCR genotyping allowed discrimination of 12 virulent E. coli isolates into 11 ERIC-PCR genotypes. The numerical index of discrimination was 0.999.
Conclusion: Our findings provide information about the wide genetic diversity and discrimination of virulent E. coli in apparently healthy poultry and poultry farm workers of Andhra Pradesh (India) based on their genotype.

Keywords

Escherichia coli, Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction, Genotype, Poultry, Serotype, Virulent.
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  • Genotyping of Virulent Escherichia coli Obtained from Poultry and Poultry Farm Workers Using Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction

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Authors

M. Soma Sekhar
Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Sri Venkateswara Veterinary University, Gannavaram, Andhra Pradesh, India
N. Mohammad Sharif
Department of Veterinary Microbiology, College of Veterinary Science, Tirupati, Andhra Pradesh, India
T. Srinivasa Rao
Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Sri Venkateswara Veterinary University, Gannavaram, Andhra Pradesh, India
M. Metta
Department of Animal Genetics and Breeding, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India

Abstract


Aim: The aim of this study was to characterize virulent Escherichia coli isolated from different poultry species and poultry farm workers using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) genotyping.
Materials and Methods: Fecal swabs from different poultry species (n=150) and poultry farm workers (n=15) were analyzed for E. coli and screened for virulence genes (stx1, stx2, eaeA, and hlyA) by multiplex PCR. Virulent E. coli was serotyped based on their “O” antigen and then genotyped using ERIC-PCR.
Results: A total of 134 E. coli isolates (122/150 from poultry and 12/15 from farm workers) were recovered. Virulence genes were detected in a total of 12 isolates. Serological typing of the 12 virulent E. coli revealed nine different serotypes (O2, O49, O60, O63, O83, O101, O120, UT, and Rough). ERIC-PCR genotyping allowed discrimination of 12 virulent E. coli isolates into 11 ERIC-PCR genotypes. The numerical index of discrimination was 0.999.
Conclusion: Our findings provide information about the wide genetic diversity and discrimination of virulent E. coli in apparently healthy poultry and poultry farm workers of Andhra Pradesh (India) based on their genotype.

Keywords


Escherichia coli, Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction, Genotype, Poultry, Serotype, Virulent.