Veterinary World

Open Access Open Access  Restricted Access Subscription Access

Semi-Nested Polymerase Chain Reaction-Based Detection of Babesia spp. in Small Ruminants from Northwest of Iran


Affiliations
1 Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran, Islamic Republic of
2 Department of Pathobiology, University of Tabriz, Tabriz, Iran, Islamic Republic of
3 Department of Clinical Sciences, University of Tabriz, Tabriz, Iran, Islamic Republic of
 

Aim: The present study aimed to detect Babesia ovis and Babesia motasi in the blood samples of sheep and goats from Northwest of Iran by the semi-nested polymerase chain reaction (PCR) technique.

Materials and Methods: A total of 166 whole blood samples (including 123 sheep and 43 goats) were collected. In the first stage, the PCR was performed to amplify a piece of 18S rRNA gene of Babesia and Theileria genera. Then, semi-nested PCR was carried out on all PCR products to differentiate B. ovis and B. motasi.

Results: The PCR indicated that totally, 19 (11.44%) out of 166 samples were positive for Babesia or Theileria spp. The semi-nested PCR showed that 38 samples (22.89%) were positive only for B. ovis. No significant association was found between the infection rate of B. ovis and age, gender and species of animals.

Conclusion: In the present study, there was no evidence for B. motasi infection in small ruminants from Northwest of Iran. Therefore, B. ovis was the main causative agent of ovine Babesiosis in this region.


Keywords

Babesia motasi, Babesia ovis, Iran, Semi-Nested Polymerase Chain Reaction, Small Ruminants.
User
Notifications
Font Size

Abstract Views: 145

PDF Views: 0




  • Semi-Nested Polymerase Chain Reaction-Based Detection of Babesia spp. in Small Ruminants from Northwest of Iran

Abstract Views: 145  |  PDF Views: 0

Authors

Ahad Bazmani
Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran, Islamic Republic of
Amir Abolhooshyar
Department of Pathobiology, University of Tabriz, Tabriz, Iran, Islamic Republic of
Imani-Baran Abbas
Department of Pathobiology, University of Tabriz, Tabriz, Iran, Islamic Republic of
Hamid Akbari
Department of Clinical Sciences, University of Tabriz, Tabriz, Iran, Islamic Republic of

Abstract


Aim: The present study aimed to detect Babesia ovis and Babesia motasi in the blood samples of sheep and goats from Northwest of Iran by the semi-nested polymerase chain reaction (PCR) technique.

Materials and Methods: A total of 166 whole blood samples (including 123 sheep and 43 goats) were collected. In the first stage, the PCR was performed to amplify a piece of 18S rRNA gene of Babesia and Theileria genera. Then, semi-nested PCR was carried out on all PCR products to differentiate B. ovis and B. motasi.

Results: The PCR indicated that totally, 19 (11.44%) out of 166 samples were positive for Babesia or Theileria spp. The semi-nested PCR showed that 38 samples (22.89%) were positive only for B. ovis. No significant association was found between the infection rate of B. ovis and age, gender and species of animals.

Conclusion: In the present study, there was no evidence for B. motasi infection in small ruminants from Northwest of Iran. Therefore, B. ovis was the main causative agent of ovine Babesiosis in this region.


Keywords


Babesia motasi, Babesia ovis, Iran, Semi-Nested Polymerase Chain Reaction, Small Ruminants.