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Protective Effect of Rutin in Comparison to Silymarin Against Induced Hepatotoxicity in Rats


Affiliations
1 Department of Veterinary Pharmacology and Toxicology, P V Narsimha Rao Telangana Veterinary University, College of Veterinary Science, Rajendranagar, Hyderabad-500030, Telangana, India
2 Department of Veterinary Pathology, P V Narsimha Rao Telangana Veterinary University, College of Veterinary Science, Rajendranagar, Hyderabad-500030, Telangana, India
 

Aim: The aim of this study is to evaluate the hepatoprotective effect of rutin (RTN) in comparison to silymarin (SLM) against acetaminophen (APAP)-induced hepatotoxicity in rats.
Materials and Methods: Male Wistar albino rats (n=24) of 3 months age were equally divided into four groups. Group 1 served as normal control. Hepatotoxicity was induced in the remaining three groups with administration of 500 mg/kg po APAP from day 1-3. Groups 2, 3, and 4 were subsequently administered orally with distilled water, 25 mg/kg of SLM, and 20 mg/kg of RTN, respectively, for 11 days. The mean body weights and biomarkers of hepatotoxicity were estimated on day 0, 4 (confirmation of toxicity), and 15 (at the end of treatment). Hematological parameters were evaluated on day 4 and 15. Antioxidant profile and adenosine triphosphatases (ATPases) were assessed at the end of the experiment. Liver tissues were subjected to histopathology and transmission electron microscopy after the sacrifice on day 15.
Results: Antioxidant profile, ATPases, and hematological and sero-biochemical parameters were significantly altered, and histopathological changes were noticed in the liver of toxic control group. These changes were reversed in groups 3 and 4 that were administered with SLM and RTN, respectively.
Conclusion: The results of the present investigation enunciated that SLM has potent hepatoprotective activity though the RTN was found superior in restoring the pathological alterations in paracetamol-induced hepatotoxicity in Wistar albino rats.

Keywords

Acetaminophen, Hepatotoxicity, Rutin, Silymarin.
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  • Kim, M.T., Huang, R., Sedykh, A., Wang, W., Xia, M. and Zhu, H. (2016) Mechanism profiling of hepatotoxicity caused by oxidative stress using antioxidant response element reporter gene assay models and big data. Environ. Health Perspect., 124(5): 634-641.

  • Kosanam, S. and Boyina, R. (2015) Drug-induced liver injury: A review. Int. J. Pharmacol. Res., 5(2): 24-30.

  • Mroueh, M., Saab, Y. and Rizkallah, R. (2004) Hepatoprotective activity of Centaurium erythrea on acetaminophen-induced hepatotoxicity in rats. Phytother. Res., 18: 431-433.

  • Hinson, J.A., Roberts, D.W. and James, L.P. (2010) Mechanisms of acetaminophen-induced liver necrosis. Hand Exp. Pharmacol., 196: 369-405.

  • Aycan, I.O., Tufek, A., Tokgoz, O., Evliyaoglu, O., Fırat, U., Kavak, G.O., Turgut, H. and Yuksel, M.U. (2014) Thymoquinone treatment against acetaminophen-induced hepatotoxicity in rats. Int. J. Surg., 12: 213-218.

  • Zhang, S., Lu, B., Han, X., Xu, L., Qi, Y., Yin, L., Xu, Y., Zhao, Y., Liu, K. and Peng, J. (2013) Protection of the flavonoid fraction from Rosa laevigata Michx fruit against carbon tetrachloride-induced acute liver injury in mice. Food Chem. Toxicol., 55: 60-69.

  • Olaleye, M.T., Amobonye, A.E., Komolafe, K. and Akinmoladun, A.C. (2014) Protective effects of Parinari curatellifolia flavonoids against acetaminophen-induced hepatic necrosis in rats. Saudi J. Biol. Sci., 21: 486-492.

  • Pattanayak, S., Nayak, S.S., Panda, D.P., Dinda, S.C., Shende, V. and Jadav, A. (2011) Hepatoprotective activity of crude flavonoids extract of Cajanus scarabaeoides (l) in paracetamol intoxicated albino rats. Asian J. Pharm. Biol. Res., 1: 22-27.

  • Luper, S. (1998) A review of plants used in the treatment of liver diseases: Part 1. Altern. Med. Rev., 3: 410-421.

  • Vargas-Mendoza, N., Madrigal-Santillan, E., MoralesGonzalez, A., Esquivel-Soto, J., Esquivel-Chirino, C., Gonzalez-Rubio, M.G.L., Gayosso-de-Lucio, J.A. and Morales-Gonzalez, J.A. (2014) Hepatoprotective effect of silymarin. World J. Hepatol., 6(3): 144-149.

  • Cacciapuoti, F., Scognamiglio, A., Palumbo, R., Forte, R. and Cacciapuoti, F. (2013) Silymarin in non-alcoholic fatty liver disease. World J. Hepatol., 5(3): 109-113.

  • Polyak, S.J., Ferenci, P. and Pawlotsky, J.M. (2013) Hepatoprotective and antiviral functions of Silymarin components in HCV infection. Hepatology, 57(3): 1262-1271.

  • Ashraf, J., Nagma, J.S., Nagma, M., Mirani, N. and Rub, A. (2012) Protective effect of rutin against carbon tetrachloride-induced hepatotoxicity in mice. Int. J. Drug. Dev. Res., 4(2): 352-357.

  • Luo, H., Jiang, B.H., King, S.M. and Chen, Y.C. (2008) Inhibition of cell growth and VEGF expression in ovarian cancer cells by flavonoids. Nutr. Cancer, 60: 800-809.

  • Sreedevi, C.D., Latha, P.G., Ancy, P., Suja, S.R., Shyamal, S., Shine, V.J., Sini, S., Anuja, G.I. and Rajasekharan, S. (2009) Hepatoprotective studies on Sida acuta Burn. J. Ethnopharmacol., 124: 171-175.

  • Madesh, M. and Balasubramanian, K.A. (1998) Micro titer plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J. Biochem. Biophys., 35(3): 184-188.

  • Asru, K.S. (1972) Colorimetric assay of catalase. Anal. Biochem., 47: 389-394.

  • Moron, M.S., Depierre, J.W. and Mannervik, B. (1979) Levels of glutathione, glutathione reductase and glutathione S transferase in rat lung and liver. Biochim. Biophys. Acta, 582: 67-68.

  • Paglia, D.E. and Valentine, W.N. (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med., 70: 158-169.

  • Balasubramanian, K.A., Manohar, M. and Mathan, V.I. (1988) An unidentified inhibitor of lipid peroxidation in intestinal mucosa. Biochim. Biophys. Acta, 962: 51-58.

  • Singh, U.B. and Sulochana, S. (1997) Handbook of Histopathological and Histochemical Techniques. 2nd ed. Premier Publishing House, Hyderabad, India. p5-41.

  • Bozzola, J.J. and Russell, L.D. (1999) Electron Microscopy: Principles and Techniques for Biologists. 2nd ed. Jones and Bartlett, Boston. p54-67.

  • Duncan, D.B. (1955) Multiple F test. Biometrics., 1: 142.

  • Senthilkumar, R., Chandran, R. and Parimelazhagan, T. (2014) Hepatoprotective effect of Rhodiola imbricata rhizome against paracetamol-induced liver toxicity in rats. Saudi J. Biol. Sci., 21(5): 409-416.

  • Mamat, S.S., Kamarolzaman, M.F.F., Yahya, F., Mahmood, N.D., Shahril, M.S., Jakius, K.F., Mohtarrudin, N., Ching, S.M., Susanti, D., Taher, M. and Zakaria, Z.M. (2013) Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats. BMC Complement. Altern. Med., 13: 326.

  • Kiran, P.M., Raju, A.V. and Rao, B.G. (2012) Investigation of hepatoprotective activity of Cyathea gigantea (Wall. ex. Hook.) leaves against paracetamol-induced hepatotoxicity in rats. Asian Pac. J. Trop. Biomed., 2(5): 352-356.

  • Saito, C., Zwingman, C. and Jaeschke, H. (2010) Novel mechanisms of protection against acetaminophen hepatotoxicity in mice by glutathione and N-acetyl cysteine. Hepatology, 51: 246-254.

  • Wendel, A., Feuerstein, S. and Konz, K.H. (1979) Acute paracetamol intoxication of starved mice leads to peroxidation in vivo. Biochem. Pharmacol., 28: 2051-2055.

  • Mandade, R.J. (2011) Hepatoprotective activity of ethanolic extract of Caesalpinia bonduc (L.) in paracetamol intoxicated Albino rats. Int. J. PharmTech. Res., 3(1): 430-437.

  • Singh, A., Bhat, T.K. and Sharma, O.P. (2011) Clinical biochemistry of hepatotoxicity. J. Clin. Toxicol., 4: 1-19.


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  • Protective Effect of Rutin in Comparison to Silymarin Against Induced Hepatotoxicity in Rats

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Authors

M. Kasi Reddy
Department of Veterinary Pharmacology and Toxicology, P V Narsimha Rao Telangana Veterinary University, College of Veterinary Science, Rajendranagar, Hyderabad-500030, Telangana, India
A. Gopala Reddy
Department of Veterinary Pharmacology and Toxicology, P V Narsimha Rao Telangana Veterinary University, College of Veterinary Science, Rajendranagar, Hyderabad-500030, Telangana, India
B. Kala Kumar
Department of Veterinary Pharmacology and Toxicology, P V Narsimha Rao Telangana Veterinary University, College of Veterinary Science, Rajendranagar, Hyderabad-500030, Telangana, India
D. Madhuri
Department of Veterinary Pathology, P V Narsimha Rao Telangana Veterinary University, College of Veterinary Science, Rajendranagar, Hyderabad-500030, Telangana, India
G. Boobalan
Department of Veterinary Pharmacology and Toxicology, P V Narsimha Rao Telangana Veterinary University, College of Veterinary Science, Rajendranagar, Hyderabad-500030, Telangana, India
M. Anudeep Reddy
Department of Veterinary Pharmacology and Toxicology, P V Narsimha Rao Telangana Veterinary University, College of Veterinary Science, Rajendranagar, Hyderabad-500030, Telangana, India

Abstract


Aim: The aim of this study is to evaluate the hepatoprotective effect of rutin (RTN) in comparison to silymarin (SLM) against acetaminophen (APAP)-induced hepatotoxicity in rats.
Materials and Methods: Male Wistar albino rats (n=24) of 3 months age were equally divided into four groups. Group 1 served as normal control. Hepatotoxicity was induced in the remaining three groups with administration of 500 mg/kg po APAP from day 1-3. Groups 2, 3, and 4 were subsequently administered orally with distilled water, 25 mg/kg of SLM, and 20 mg/kg of RTN, respectively, for 11 days. The mean body weights and biomarkers of hepatotoxicity were estimated on day 0, 4 (confirmation of toxicity), and 15 (at the end of treatment). Hematological parameters were evaluated on day 4 and 15. Antioxidant profile and adenosine triphosphatases (ATPases) were assessed at the end of the experiment. Liver tissues were subjected to histopathology and transmission electron microscopy after the sacrifice on day 15.
Results: Antioxidant profile, ATPases, and hematological and sero-biochemical parameters were significantly altered, and histopathological changes were noticed in the liver of toxic control group. These changes were reversed in groups 3 and 4 that were administered with SLM and RTN, respectively.
Conclusion: The results of the present investigation enunciated that SLM has potent hepatoprotective activity though the RTN was found superior in restoring the pathological alterations in paracetamol-induced hepatotoxicity in Wistar albino rats.

Keywords


Acetaminophen, Hepatotoxicity, Rutin, Silymarin.

References