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Transcriptomic Comparison of Primary Bovine Horn Core Carcinoma Culture and Parental Tissue at Early Stage


Affiliations
1 Veterinary Officer (WBAH & VS), West Bengal Animal Resources Development Department, Bankura-772152, West Bengal, India
2 Department of Animal Genetics & Breeding, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
3 Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
4 Hester Biosciences Limited, Ahmedabad, Gujarat, India
5 Veterinary Officer, MVC Sarenga, Government of West Bengal, Bankura, West Bengal, India
 

Aim: Squamous cell carcinoma or SCC of horn in bovines (bovine horn core carcinoma) frequently observed in Bos indicus affecting almost 1% of cattle population. Freshly isolated primary epithelial cells may be closely related to the malignant epithelial cells of the tumor. Comparison of gene expression in between horn’s SCC tissue and its early passage primary culture using next generation sequencing was the aim of this study.
Materials and Methods: Whole transcriptome sequencing of horn’s SCC tissue and its early passage cells using Ion Torrent PGM were done. Comparative expression and analysis of different genes and pathways related to cancer and biological processes associated with malignancy, proliferating capacity, differentiation, apoptosis, senescence, adhesion, cohesion, migration, invasion, angiogenesis, and metabolic pathways were identified.
Results: Up-regulated genes in SCC of horn’s early passage cells were involved in transporter activity, catalytic activity, nucleic acid binding transcription factor activity, biogenesis, cellular processes, biological regulation and localization and the down-regulated genes mainly were involved in focal adhesion, extracellular matrix receptor interaction and spliceosome activity.
Conclusion: The experiment revealed similar transcriptomic nature of horn’s SCC tissue and its early passage cells.

Keywords

Cummerbund, Gene Ontology, Primary Culture, RNA-Sequencing, Squamous Cell Carcinoma of Horn, Transcriptome Profiling.
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  • Transcriptomic Comparison of Primary Bovine Horn Core Carcinoma Culture and Parental Tissue at Early Stage

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Authors

Sharadindu Shil
Veterinary Officer (WBAH & VS), West Bengal Animal Resources Development Department, Bankura-772152, West Bengal, India
R. S. Joshi
Department of Animal Genetics & Breeding, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
C. G. Joshi
Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
A. K. Patel
Hester Biosciences Limited, Ahmedabad, Gujarat, India
Ravi K. Shah
Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
Namrata Patel
Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
Subhash J. Jakhesara
Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
Sumana Kundu
Veterinary Officer, MVC Sarenga, Government of West Bengal, Bankura, West Bengal, India
Bhaskar Reddy
Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
P. G. Koringa
Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
D. N. Rank
Department of Animal Genetics & Breeding, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India

Abstract


Aim: Squamous cell carcinoma or SCC of horn in bovines (bovine horn core carcinoma) frequently observed in Bos indicus affecting almost 1% of cattle population. Freshly isolated primary epithelial cells may be closely related to the malignant epithelial cells of the tumor. Comparison of gene expression in between horn’s SCC tissue and its early passage primary culture using next generation sequencing was the aim of this study.
Materials and Methods: Whole transcriptome sequencing of horn’s SCC tissue and its early passage cells using Ion Torrent PGM were done. Comparative expression and analysis of different genes and pathways related to cancer and biological processes associated with malignancy, proliferating capacity, differentiation, apoptosis, senescence, adhesion, cohesion, migration, invasion, angiogenesis, and metabolic pathways were identified.
Results: Up-regulated genes in SCC of horn’s early passage cells were involved in transporter activity, catalytic activity, nucleic acid binding transcription factor activity, biogenesis, cellular processes, biological regulation and localization and the down-regulated genes mainly were involved in focal adhesion, extracellular matrix receptor interaction and spliceosome activity.
Conclusion: The experiment revealed similar transcriptomic nature of horn’s SCC tissue and its early passage cells.

Keywords


Cummerbund, Gene Ontology, Primary Culture, RNA-Sequencing, Squamous Cell Carcinoma of Horn, Transcriptome Profiling.

References