South Indian Journal of Biological Sciences

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Comparative Effects of Plant Growth Regulators on Callus Induction in Leaf Explants of Wedelia chinensis


Affiliations
1 KVM College of Engineering & Information Technology, Cherthala, Alappuzha, Kerala, India
2 Greenova Center for Botanical Innovation, Trivandrum, Kerala, India
3 Department of Biotechnology, Kamaraj college of Engineering and Technology, Virudhunagar, Tamilnadu, India
 

Wedelia chinensis, a perennial herb of the family Asteraceae, is used traditionally in ayurvedic, siddha and Unani system of medicines. The plant has been extensively used in inflammations, helmintic diseases and liver disorders. This excessive pharmaceutical application has led to serious shortage of the plant in natural habitat. In vitro culture technique has been proved to be very effective for rapid propagation of rare plant species including medicinal plants. Callus induction and study of callogenic response to different plant growth regulators is a major step in production of phytoconstituents using invitro techniques. The present study was therefore undertaken with a view to develop an efficient and repeatable protocol for rapid production of callus cultures for the production of these phytochemical constituents. Leaf segments were used as explants and were cultured on Murashige and Skoog's (MS) medium supplemented with different concentrations of auxins (2, 4‐dichlorophenoxyacetic acid (2,4‐D), indole acetic acid (IAA)) alone and in combination with different cytokinins (6‐benzylaminopurine (6‐BAP), kinetin (Kn)). The callus obtained were friable and greenish white in colour. Maximum rate of callus formation was observed in media containing 1 mg/L 2,4‐D and 0.5 mg/L 6‐BAP.

Keywords

Wedelia chinensis, Murashige And Skoog Medium, 6-Benzyl Amino Purine, Kinetin.
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  • Comparative Effects of Plant Growth Regulators on Callus Induction in Leaf Explants of Wedelia chinensis

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Authors

Brijit C. Dhas
KVM College of Engineering & Information Technology, Cherthala, Alappuzha, Kerala, India
Nimmi S. Simon
KVM College of Engineering & Information Technology, Cherthala, Alappuzha, Kerala, India
P. Austin
Greenova Center for Botanical Innovation, Trivandrum, Kerala, India
Sentamil Selvan
Department of Biotechnology, Kamaraj college of Engineering and Technology, Virudhunagar, Tamilnadu, India
Shyam Kumar Rajaram
Department of Biotechnology, Kamaraj college of Engineering and Technology, Virudhunagar, Tamilnadu, India
Ronaldo Anuf Alexander
Department of Biotechnology, Kamaraj college of Engineering and Technology, Virudhunagar, Tamilnadu, India

Abstract


Wedelia chinensis, a perennial herb of the family Asteraceae, is used traditionally in ayurvedic, siddha and Unani system of medicines. The plant has been extensively used in inflammations, helmintic diseases and liver disorders. This excessive pharmaceutical application has led to serious shortage of the plant in natural habitat. In vitro culture technique has been proved to be very effective for rapid propagation of rare plant species including medicinal plants. Callus induction and study of callogenic response to different plant growth regulators is a major step in production of phytoconstituents using invitro techniques. The present study was therefore undertaken with a view to develop an efficient and repeatable protocol for rapid production of callus cultures for the production of these phytochemical constituents. Leaf segments were used as explants and were cultured on Murashige and Skoog's (MS) medium supplemented with different concentrations of auxins (2, 4‐dichlorophenoxyacetic acid (2,4‐D), indole acetic acid (IAA)) alone and in combination with different cytokinins (6‐benzylaminopurine (6‐BAP), kinetin (Kn)). The callus obtained were friable and greenish white in colour. Maximum rate of callus formation was observed in media containing 1 mg/L 2,4‐D and 0.5 mg/L 6‐BAP.

Keywords


Wedelia chinensis, Murashige And Skoog Medium, 6-Benzyl Amino Purine, Kinetin.

References