Research Journal of Science and Technology

S. Deepa ¹, K. Kanimozhi ², A. Panneerselvam ²

Affiliations
1 P.G. Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous) Poondi-613503, Thanjavur Dist, Tamil Nadu, IN
2 P.G. Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous) Poondi-613503, Thanjavur Dist, Tamil Nadu, IN

Abstract

The soil sample was collected from the salt pan of Bay of Bengal at Vedaranyam,Nagapattinam (Dt.) Tamilnadu, India. The physico-chemical parameters at the sample were analyzed by using standard procedures.Totally 10 actinomycetes were isolated and identified from the salt pan soil sample. Two actinomycetes namely Jonesia denitrificans and Nocardia amarae were dominant in their growth out of 10 isolated actinomycetes.

Antibacterial activity of Jonesia denitrificans and Nocardia amarae were studied against five human bacterial pathogens such as Salmonella typhi, Bacillus subtilis, Staphylococcus aureus, Vibrio cholerae and Klebsiella pneumonia. The acetone extract of Nocardia amarae exhibited promising antibacterial activity against Bacillus subtilis (25 mm). The isolation, characterization and the study on actinomycetes can be useful in the discovery of antibiotics.

Keywords

Antibacterial Activity, Marine Actinomycetes, Jonesia denitrificans.

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S. Deepa ¹, P. Bhuvanehswari ², K. Kanimozhi ¹, A. Panneerselvam ¹

Affiliations
1 PG and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College, (Autonomous), Poondi, Thanjavur, IN
2 Department of Microbiology, A.V.C College (Autonomous), Mannampandal, Mayiladuthurai, IN

Abstract

Alcaligenes eutrophus was collected from MTCC bank and purity was checked according to the guidelines of Bergey's manual of bacteriology. The PHB production ability of the culture was tested by "Granular stains method". The test organism was grown in synthetic and various industrial waste based medium (Molasses, seasame oil waste) at different concentration (10%, 20%, 30% and 40%).The PHB producer grown in synthetic and various industrial waste based medium and PHB was extracte and estimated by using UV spectrophotometer. The pure form of PHB powder was collected and studied by Infrared analysis. In the present study, the high PHB production was seen in Alcaligenes eutrophus grown in 40% concentration of seasame oil waste when compared with other substrate and synthetic medium. The present study concludes that the seasame oil waste based medium and Alcaligenes eutrophus used for PHB production in large scale that minimize the cost of the product. Thus we can minimize the use of petroleum-derived plastics and by which we can live in a plastic pollution free earth.

Keywords

PHB, Molasses, Seasame Oil Waste, Alcaligenes eutrophus.

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