Research Journal of Science and Technology

S. Sangeetha ¹, R. Anuradha ²

Affiliations
1 PG and Research Department of Biochemistry, S.T.E.T. Women’s College, Sundarakkottai, Mannargudi -614016, Tamilnadu, IN
2 PG and Research Department of Biochemistry, S.T.E.T. Women’s College, Sundarakkottai, Mannargudi-614016, Tamil Nadu, IN

Abstract

The present study was carried out to determine the production of the amylase enzyme from Aspergillus niger under submerged fermentation method. Amylase containing protein content was estimated. Effect of Ph, temperature and substrate concentration of amylase activity were also monitered during amylase production. The enzyme was active at pH 4 and 40°C. A maximum amylase production 15.20 IU/ml in rice bran and 13.05 IU/ml in potato skin waste medium was obtained at 48 hours under optimum conditions.

Keywords

Aspergillus niger, Rice Bran, Potato Skin, P^H, Temperature, Protein.

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