Research Journal of Science and Technology

A. Vinoth ¹, V. Balakrishnan ¹, R. Kalaivani ¹, P. Madhanraj ², N. Nadimuthu ³

Affiliations
1 Department of Biotechnology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
2 Indian Biotrack Research Institute, Thanjavur, Tamil Nadu, IN
3 P.G. Department of Plant Science, Avvaiyar Government College for Women, Karaikal 609 602, Puducherry U.T., IN

Abstract

Oil pollution is worldwide major problem in every environment and hence the world is need of a perfect solution for prevention or recovery so, the present investigation was carried out to detect the spent lubricant oil degradation potentional of indigenous fungi in lab level by measuring the growth and analysis the treated sample using FTIR. Soil sample was randomly collected in the different location at railway tracks in Thanjavur junction soil sample are serially and 10^-2 is used for plating technique in PDA medium. After incubation the isolates were obtained and they are Aspergillus terreus, Aspergillus flavus and Aspergillus niger. Oil degrading ability was detected supplying raw spent oil in PDA medium and incoprporated in PD broth at the concentration of (5%.10%, 15% and 20%), detection shows that no fungal cored use raw oil as source of nutrient. In PDA plate's visual detection of zone due to the degradation was noticed. It was maximum by Aspergillus flavus follwed by Aspergillus terreus and Aspergillus niger. In PD broth supplemented with different spent oil concentration (5%,10%,15% and 20%), after 14 days of incubation (30°C), as a visual the biomass of each fungal culture was determined the biomass of different species of fungi in different concentration oil showed growth but with variation the higher mycelial biomass was recorded by Aspergillus flavus. The FT-IR analysis results shows that is major difference in the peak formation between the tested samples which shows that fungal species has utilized or degraded the oil hydrocarbon. Differently as per their metabolize activity.

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S. Suganya ¹, R. Bharathidasan ¹, G. Senthilkumar ¹, P. Madhanraj ², A. Panneerselvam ¹

Affiliations
1 PG and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi, Thanjavur, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN

Abstract

Extraction of essential oils from locally available plant Coriandrum sativam (L.) was carried out using steam distillation followed by ether extraction. Dried and purified extracted oils were screened for their antibacterial activity of both gram positive (Staphylococcus aureus) and gram negative (Enterobacter aerogenes, Klebsiella pneumoniae, Vibrio cholerae and Salmonella typhi) bacterial strains by Agar well diffusion method. Coriandrum sativam shows antibacterial activity against the above given five bacterial strains. GC-MS was done on Coriandrum sativam oil. These results support that this plant oil can be used to cure bacterial infections and may also have role as pharmaceuticals and preservatives.

Keywords

Coriandrum sativam, GC-MS, Essential Oil, Antibacterial Activity and Extraction.

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P. Madhanraj ¹, S. Manorajan ², N. Nadimuthu ³, A. Panneerselvam ⁴

Affiliations
1 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
2 Biology Education Centre, Uppsala University, SE-752 36 Uppsala, SE
3 Dept. of Plant Science, Avvaiyar Government College for Women, Karaikal – 609 602, Pondicherry, U.T., IN
4 Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613 503, Thanjavur (Dt.), Tamilnadu, IN

Abstract

In seasonally inundated sediments of Manianthivu of Nagapattinam district, southeast coast of India, a total number of 24 species belonged to 5 genera were recorded. They were assignable to Phycomycete (1 species) and Deuteromycetes (23 species). They exhibited spatio-temporal changes. The genus Aspergillus was constituted by the maximum of 19 species followed by Trichoderma (2 species). All other genera (Absidia, Botrytis and Fusarium) were represented by one species each. The soil characteristics such as pH (8.1 to 8.9), electrical conductivity (0.11 to 1.28 dSm^-1), cation exchange capacity (8.01 to 9.5 c.mol proton⁺/kg), organic carbon (0.05 to 0.18%), available nitrogen (0.012 to 0.018%), available phosphorus (0.002 to 0.005%), available potassium (0.013 to 0.047 ppm), available zinc (0.39 to 0.57 ppm), available iron (2.12 to 2.83 ppm), available copper (0.19 to 0.31 ppm), available manganese (1.41 to 1.52 ppm), calcium (3.2 to 5.2 mg/kg), magnesium(3.2 to 3.9 mg/kg), sodium (0.52 to 1.22 mg/kg) and potassium (0.02 to 0.07 mg/kg) were also showed variation during different seasons. The investigation was carried out by collections and examination of sediment samples during January 2007 - December 2008, at seasonal intervals.

Keywords

Fungi, Soil Characteristics, Inundated Sediment.

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K. Kaviyarasi ¹, A. Cholarajan ², R. Bharathidasan ², P. Madhanraj ², A. Panneerselvam ²

Affiliations
1 P.G. and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi -613503, Thanjavur, Tamilnadu, IN
2 P.G. and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi -613503, Thanjavur, Tamilnadu, IN

Abstract

PGPR offers an environmentally sustainable approach to crop production and health. Many members of genera pseudomonas sp. perform beneficial effects on plants. Among them P. putida and P. fluorescens are fluorescent pseudomonas that represent Significant part of these Members. The rhizosphere soil was collected from cultivated plots. P. fluorescence was isolated and identified through morphologicaland hemical tests and it was treated against some plant pathogens. The biocontrol potential of P. fluorescens against plant pathogen such as A. terreus sp, Circinella sp, Verticillium sp, was assessed by dual plate technique. The seed treatment was made on four plants namely Hibiscus esculentus, Oryza sativa, Capsicum annum, Spinacia olerace. Pot culture techniques was applied for the growth of plants along with p. fluorescence inoculums (100ml) and chemical fertilizer (10g).The plant height, ischolar_main length, ischolar_main biomass, shoot biomass were also estimated. The results were discussed.

Keywords

PGPR, Pseudomonas fluorescence.

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A. Prathiba ¹, P. Madhanraj ², A. Panneerselvam ¹

Affiliations
1 Department of Botany and Microbiology, A. V. V. M Sri Pushpam College, Poondi – 613 503, Thanjavur District, Tamil Nadu, IN
2 Department of Botany and Microbiology, A. V. V. M Sri Pushpam College, Poondi – 613 503, Thanjavur District, Tamil Nadu., IN

Abstract

In the present investigation, the mushroom yield, chemical composition, nutritional value of Pleurotus platypus cultivated in paddy straw, black gram stem and black gram pods were determined. Fresh mushroom yield amounts obtained from paddy straw, black gram stem and black gram pods substrate were 940, 560 and 595 g / kg respectively. Mushroom cultivated on different substrates were analyzed for protein, carbohydrate, amino acids and fatty acid content. The cellulolytic activity and coir pith compost was also investigated in Pleurotus platypus. Hence P. platypus was also selected for molecular studies. Therefore, the present results suggest it could be used to recycle the agro – waste materials.

Keywords

Pleurotus platypus, Chemical Composition, Nutritive Value, Cellulolytic Activity, Coir Pith Compost.

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