Research Journal of Science and Technology

A. Vinoth ¹, V. Balakrishnan ¹, R. Kalaivani ¹, P. Madhanraj ², N. Nadimuthu ³

Affiliations
1 Department of Biotechnology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
2 Indian Biotrack Research Institute, Thanjavur, Tamil Nadu, IN
3 P.G. Department of Plant Science, Avvaiyar Government College for Women, Karaikal 609 602, Puducherry U.T., IN

Abstract

Oil pollution is worldwide major problem in every environment and hence the world is need of a perfect solution for prevention or recovery so, the present investigation was carried out to detect the spent lubricant oil degradation potentional of indigenous fungi in lab level by measuring the growth and analysis the treated sample using FTIR. Soil sample was randomly collected in the different location at railway tracks in Thanjavur junction soil sample are serially and 10^-2 is used for plating technique in PDA medium. After incubation the isolates were obtained and they are Aspergillus terreus, Aspergillus flavus and Aspergillus niger. Oil degrading ability was detected supplying raw spent oil in PDA medium and incoprporated in PD broth at the concentration of (5%.10%, 15% and 20%), detection shows that no fungal cored use raw oil as source of nutrient. In PDA plate's visual detection of zone due to the degradation was noticed. It was maximum by Aspergillus flavus follwed by Aspergillus terreus and Aspergillus niger. In PD broth supplemented with different spent oil concentration (5%,10%,15% and 20%), after 14 days of incubation (30°C), as a visual the biomass of each fungal culture was determined the biomass of different species of fungi in different concentration oil showed growth but with variation the higher mycelial biomass was recorded by Aspergillus flavus. The FT-IR analysis results shows that is major difference in the peak formation between the tested samples which shows that fungal species has utilized or degraded the oil hydrocarbon. Differently as per their metabolize activity.

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P. Madhanraj ¹, S. Manorajan ², N. Nadimuthu ³, A. Panneerselvam ⁴

Affiliations
1 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
2 Biology Education Centre, Uppsala University, SE-752 36 Uppsala, SE
3 Dept. of Plant Science, Avvaiyar Government College for Women, Karaikal – 609 602, Pondicherry, U.T., IN
4 Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613 503, Thanjavur (Dt.), Tamilnadu, IN

Abstract

In seasonally inundated sediments of Manianthivu of Nagapattinam district, southeast coast of India, a total number of 24 species belonged to 5 genera were recorded. They were assignable to Phycomycete (1 species) and Deuteromycetes (23 species). They exhibited spatio-temporal changes. The genus Aspergillus was constituted by the maximum of 19 species followed by Trichoderma (2 species). All other genera (Absidia, Botrytis and Fusarium) were represented by one species each. The soil characteristics such as pH (8.1 to 8.9), electrical conductivity (0.11 to 1.28 dSm^-1), cation exchange capacity (8.01 to 9.5 c.mol proton⁺/kg), organic carbon (0.05 to 0.18%), available nitrogen (0.012 to 0.018%), available phosphorus (0.002 to 0.005%), available potassium (0.013 to 0.047 ppm), available zinc (0.39 to 0.57 ppm), available iron (2.12 to 2.83 ppm), available copper (0.19 to 0.31 ppm), available manganese (1.41 to 1.52 ppm), calcium (3.2 to 5.2 mg/kg), magnesium(3.2 to 3.9 mg/kg), sodium (0.52 to 1.22 mg/kg) and potassium (0.02 to 0.07 mg/kg) were also showed variation during different seasons. The investigation was carried out by collections and examination of sediment samples during January 2007 - December 2008, at seasonal intervals.

Keywords

Fungi, Soil Characteristics, Inundated Sediment.

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