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Authors
Affiliations
1 Department of Biotechnology, Vels University,
Pallavaram, Chennai – 117, IN
2 Department of Biotechnology, Vels University, Pallavaram, Chennai – 117, IN
Source
Research Journal of Science and Technology, Vol 3, No 1 (2011), Pagination: 33-43
Abstract
Enzymes found in nature have been used since ancient times in various fields. Overall, the estimated value of the worldwide use of industrial enzymes has grown from $ 1 to $ 1.5 billion during 1995 to 2000. In this group, Manganese Peroxidase belong to Oxido-reductase group.White rot fungi are believed to be the only microorganisms that are able to selectively and efficiently degrade the total component ligninolytic enzyme. In the present study the dye degradation potential of the fungus Phanerochaete chrysosporium studied on the liquid medium. The fungus was able to decolorize azo dyes. However, the extent of color removal was not consistent with the two dyes. The manganese peroxidase shows positive reaction in the liquid culture. This indicates that the dyes degradation occurred via enzymatic oxidation. The culture condition like incubation period, pH temperature, carbon and nitrogen sources greatly influence the growth and enzyme production of lignin degrading fungi. The pH of the culture medium is critical to the growth, ligninolytic enzyme production and xenobiotics degradation. In the present study, pH 6 was found suitable for the maximum growth and manganese peroxidase production of Phanerochaete chrysosporium. Most of the white rot fungi produce lignolytic enzyme in response to carbon, nitrogen and sulphur limitations In accordance with earlier findings, in present study Phanerochaete chrysosporium shows maximum manganese peroxidase production at higher concentration of glucose 20g/l Nitrogen source exerts a great influence on the extracellular lignolytic enzyme production of wood rotting Basidiomycetes. In the present study, among the different nitrogen sources Ammonium tartrate favored maximum manganese peroxidase enzyme production. In the present study production of manganese peroxidase was enhanced by several folds by solid state fermentation than submerged cultures. Among the two different substrate used, wheat bran highly supported the mycelial growth and manganese peroxidase production than other substrate such as rice straw.
Keywords
Phanerochaete chrysosporium, Azo Dye, Solid State Fermentation, Submerged Culture, Manganese peroxidase.
