Research Journal of Science and Technology

Afaque Quraishi ¹

Affiliations
1 School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, IN

Abstract

Acacia mangium, is a fast growing leguminous tree. It forms hybrids with A. auriculiformis, by air pollination; thus, micropropagation may provide genetically pure quality planting material. Therefore in vitro cultures were initiated from nodal explants of 1-year-old Mangium plant, on Driver and Kuniyuki medium with 6-benzyl adenine and polyvinyl pyrrolidone. Shoot cultures were further proliferated using on very low level of 6-benzyl adenine. Different adjuvant were tested for shoot multiplication. Shoot cultures were maintained up to 1 ½ years with retaining the shoot proliferation capacity, by normal subculture cycles. These 1 ½ years old shoot cultures were successfully ischolar_mained in vitro on half strength medium in presence of auxin, to regenerate complete plants.

Keywords

Micropropagation, DKW Medium, Propagule Proliferation, Adjuvant, Subculture, Rooting.

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