Research Journal of Science and Technology

G. Vaijayanthi ¹, A. Cholarajan ², R. Vijayakumar ³

Affiliations
1 P.G. Department of Microbiology, Meenakshi Chandrasekaran College of Arts and Science, Pattukkottai – 614 626, Tamil Nadu, IN
2 P.G. Department of Microbiology, Meenakshi Chandrasekaran College of Arts and Science Pattukkottai-614 626, Tamil Nadu, IN
3 Department of Microbiology, Bharathidasan University College for Women, Orathanadu-614 625, Tamil Nadu, IN

Abstract

The terrestrial soil sample was collected from Orathanadu, Thanjavur (Dt.), Tamilnadu, India. Physical and chemical characteristics of soil samples such as pH, electrical conductivity (EC), calcium (Ca2+), magnesium (Mg2+), bicarbonate (HCO3^-), chloride (Cl^-), total organic carbon, available nitrogen (N), phosphorus (P₂O₅) and potassium (K₂O) were analyzed. A total of 10 actinobacteria were isolated from the terrestrial soil sample and they were characterized based on the morphological and biochemical properties. Among 10 isolates, each one was belonged to Actinobispora sp., Actinosynnema sp., Actinoplanes sp., Agromyces sp., Actinomadura sp., Jonesia sp., Catellatospora sp., Micromonospora sp., Microtetrospora sp. and Nocardia sp. Antibacterial activity of actinobacteria were tested against five different human pathogenic bacteria such as Salmonella typhi, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Enterobacter aerogenes. Further, the purification of antibacterial compound was performed by using thin layer chromatography and the functional groups of bioactive compounds were determined by FT-IR and UV spectral studies.

Keywords

Terrestrial Soil, Actinobacteria, Physico-Chemical Analysis, Antibacterial Activity, Spectral Studies.

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K. Kaviyarasi ¹, A. Cholarajan ², R. Bharathidasan ², P. Madhanraj ², A. Panneerselvam ²

Affiliations
1 P.G. and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi -613503, Thanjavur, Tamilnadu, IN
2 P.G. and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi -613503, Thanjavur, Tamilnadu, IN

Abstract

PGPR offers an environmentally sustainable approach to crop production and health. Many members of genera pseudomonas sp. perform beneficial effects on plants. Among them P. putida and P. fluorescens are fluorescent pseudomonas that represent Significant part of these Members. The rhizosphere soil was collected from cultivated plots. P. fluorescence was isolated and identified through morphologicaland hemical tests and it was treated against some plant pathogens. The biocontrol potential of P. fluorescens against plant pathogen such as A. terreus sp, Circinella sp, Verticillium sp, was assessed by dual plate technique. The seed treatment was made on four plants namely Hibiscus esculentus, Oryza sativa, Capsicum annum, Spinacia olerace. Pot culture techniques was applied for the growth of plants along with p. fluorescence inoculums (100ml) and chemical fertilizer (10g).The plant height, ischolar_main length, ischolar_main biomass, shoot biomass were also estimated. The results were discussed.

Keywords

PGPR, Pseudomonas fluorescence.

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