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Azarakhsh, Yousef
- The Frequency of the most Potentially Toxigenic Fungi in Broiler Feeds in Kermanshah Province, West of Iran
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Authors
Affiliations
1 Department of Pathobiology, Razi University, Kermanshah, IR
2 Department of Microbiology, Faculty of Science, Islamic Azad University, Karaj Branch, Karaj, IR
3 Department of Medical and Veterinary Mycology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR
1 Department of Pathobiology, Razi University, Kermanshah, IR
2 Department of Microbiology, Faculty of Science, Islamic Azad University, Karaj Branch, Karaj, IR
3 Department of Medical and Veterinary Mycology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 3, No 1 (2011), Pagination: 11-16Abstract
The objectives of this study were to investigate the occurrence and identification of the most potentially toxigenic fungi in broiler feeds in Kermanshah province, west of Iran. From April 2008 to March 2009, a total of 100 broiler feed samples were aseptically collected from different broiler farms located in the province. Samples were transported to the laboratory, homogenized, quartered to obtain a one kg laboratory sample and were stored at 4°C for fungal analyses. Ten grams of each feed sample were homogenized in 90 ml sterile physiological saline for 30 minutes to obtain a concentration of 10-1 (dilution 1). Then this mixture was serially diluted to 10-2 (dilution 2), 10-3 (dilution 3) and 10-4 (dilution 4). From each dilution, 25μL of the mixture was deeply inoculated on dichloran rose-bengal-chloranphenicol agar(DRBC) and incubated at 25°C for 5 to 15 days. During the incubation period, gross and microscopic features of fungal colonies were studied. Statistical analyses of the data were done using SPSS software (Version 16). Aspergillus Spp. were the most prevalent; they could be isolated from 92 samples (51.1 %) in dilution 1, 76 samples (59.3%) in dilution 2, 56 samples (66.6%)m' dilution 3 and 40 samples (83%) in dilution 4. Fusarium spp. Were isolated from 52 samples (28.8%) in dilution 1, 28 samples (21.8%) in dilution 2, 20 samples (23.8%) in dilution 3 and 8 samples (16%) in dilution 4. Penicillium spp. Could be isolated from 36 samples (20%) in dilution 1, 24 samples (18.7%) in dilution 2, 8 samples (9%) in dilution 3 and 0 samples (0%)) in dilution 4. The results of this study indicate the high rate of contamination of broiler feeds to common toxigenic fungi in western parts of Iran.Keywords
Toxigenic Fungi,Mycotoxins, Broiler Feed, Kermanshah Province.
References
- Abarca, M.L., M.R. Bragulat, G. Castelhi and F.J. Cabafies,1994a Mycoflora and aflatoxin- producing strains in animal mixed feeds. J. Food Prot., 57: 256-258.
- Abarca, M.L., M.R. Bragulat, G. Castella and C. Cabafes, 1994b. Ochratoxin A production by strains of Aspergillus niger var niger. Appl. Environm. Microbiol., 60: 2650-2652.
- Abarca, M.L., F. Accensi, MR. Bragulat and F.J. Cabannes, 2001 Current importance of Ochratoxin A-producing Aspergillus spp. J. Food Protect., 64: 903-906.
- Accensi, F., M.L. Abarca, F.J. Cabannes, 2004 Occurrence of Aspergillus species in mixed feeds and component raw materials and their ability to produce ochratoxin A. Food Microbiol., 21: 623-627.
- Adebajo, L. 0., A.A. Idowu and 0. 0. Adesanya, 1994. Mycobiota and mycotoxins production in Nigeria corn and com-based snacks. Mycopathologia, 126: 183-192.
- Badripour, H.,2010Country pasture profiles. http://www.fao.org/ag/ACTP/AGPC/doc/Counprof/Ir an/Iran. htm #main.
- Bankole, S.A. and K.A. Kpodo, 2005. Mycotoxin contamination in food systems in West and Central Africa. In Reducing the impact of Mycotoxin in Tropical Agriculture with emphasis on health and trade in Africa, Acra, Ghana, pp: 13-16.
- Baron, E.J.O., L.R. Peterson and S.M. Finegold, 1994. Bailey and Scott's Diagnostic Microbiology, 9'. ed. Mosby, Baltimore.
- Bragulat, M.R., M.L. Abarca, O. Castella and J. Cabanes, 1995. Mycological survey on mixed poultry feeds and mixed rabbit feeds. J. Sci. Food and Agric., 67: 215-220.
- Burgess, L.W., B.A. Sumemerell, S. Bullock, K.P. Gott, D. Backhouse, 1994. Laboratory Manual for Fusarium Research. Fusarium Research Laboratory, Department of Crop Sciences, University of Sydney and Royal Botanic Gardens, Sydney, pp: 133.
- Chang-Yen, I., K. Bidasee and G. Rampersad, 1992. Mycoflora and mycotoxin-producing potential of fungi from poultry feeds and feed ingredients in Trinidad. J. Sci. Food Agrie., 60: 283-286.
- Cotty, J.P.,1997. Aflatoxin-producing potential communities of Aspergillus sectionflavi from cotton producing areas in the United States. Mycol. Res., 101: 698-704.
- D'Mello, J.P.F., C.M. Placinta and A.M.C. Macdonald, 1999. Fusarium mycotoxins: a review of global implications for animal health, welfare and productivity. Aim Feed Sci. Technol., 80: 183-205.
- Dalcero, A., C. Magnoli, S. Chiacchiera, G. Palacios and M. Reynoso, 1997. Mycobiota and incidence of aflatoxin BI, zearalenone and deoxinyvalenol in poultry feeds in Argentina. Mycopathologia, 137: 179-184.
- Dalcero, A., C. Magnoli, M. Luna, G. Ancasi, M.M. Reynoso, S. Chiachiera, R. Miazzo and G. Palacio, 1998. Mycoflora and naturally occurring mycotoxins in poultry feeds in Argentina. Mycopathologia, 141: 37-43.
- Frisvad, J.C. and O. Filtenborg, 1989. Terverticillate Penicillia Chemotaxonomy and Mycotoxin Production. Mycologia, 81: 837-861.
- Glenda, R., J. Oliveira, M. Ribeiro, M.E. Fraga, L.R. Cavaglieri, G.M. Direito, K.M. Keller, A.M. Dalcero and C.A. Rosa,2006. Mycobiota in poultry feeds and natural occurrence of aflatoxins, fumonisins and zearalenone in the Rio de Janeiro State, Brazil. Mycopathologia, 162: 355-362.
- Gourama, H. and L. Bullerman, 1995. Aspergillus flavus and A. parasiticus: Aflatoxigemc fungi of concern in foods and feeds: A review. J. Food Protection, 58: 1395-1404.
- Heperkan, D. and I. Alperden, 1988. Mycological survey of chicken feed and some feed ingredients in Turkey. J. Food Protect, 51: 807-810.
- Joffe, A.Z., 1983. Fusarium as field, stored and soil fungi under semiarid conditions in Israel. In: Y. Ueno, (ed.),trichothecenes-chemical, biological and toxicological aspects. Oxford, pp: 95-110.
- Khosravi, A.R., M. Dakhili and H. Shokri, 2008. A mycological survey on feed ingredients and mixed animal feeds in Ghom province, Iran, Pakistan J. Nutrition., 7: 31-34.
- King, A.D., A.D. Hocking and J.I. Pitt, 1979. Dichloran-rose bengal medium for enumeration of molds from foods. Appl. Environ. Microbiol., 37:959-964.
- Klich, M.A.,2002. Identification of Common Aspergillus Species. Utrecht, Netherlands, CBS.
- Labuda, R. and D. Tancinova, 2006. Fungi recovered in poultry feed mixture. Ann. Agri. Environ. Med., 13: 193-200.
- Lacey, J., 1991. Natural occurrence of mycotoxins in growing and conserved crops. In: J.E. Smith and R.S. Henderson, (eds), Mycotoxins and Animal Foods. CRC Press, pp: 363-390.
- Lacey, J. and N. Magan, 1991. Fungi in cereal grains, their occurrence and water and temperature relationships. In: J. Chelkowski, (ed), Cereal Grain, Mycotoxins, Fungi and Quality in Drying and Storage. Elsevier, Amsterdam, pp: 77-118.
- Lambuda, R., D. Tamcinova and K. Hudec, 2003. Identification and enumeration of Fusarium species in poultry feed mixtures from Slovakia. Annals of Agricultural and Environmental Medicine, 10: 61-66.
- Lebars-Bailly, S., J.D. Bailly and H. Brugere, 1999. Accidents de fabrication dus aux moisissures en fromagerie. Rev. Med. Vet., 150: 413-430.
- Lovett, J., 1972. Toxigenic fungi from poultry feed and litter. Poultry Sci., 51: 309-313.
- Lozada, A.F., 1995. Isolation and identification of mycotoxigenic fungi in selected foods and feeds. Food Additives and Contaminants, 3: 509-514.
- Magnoli, C., A. Dalcero, S.M. Chiacchiera, R. Miazzo and M. Sa' enz, 1998. Enumeration and identification of Aspergillus group and Penicillium species in poultry feeds in Argentina. Mycopathologia. 142: 27-32.
- Magnoli, C., S.M. Chiacchiera, R. Miazzo, G. Palacio, A. Angeletti, C. Hallak, A. Dalcero, 2002. The mycoflora and toxicity of feedstuffs from a production plant in Cordoba, Argentina. Mycotoxin Res., 18: 7-22.
- Marasas, W.F.O.,1992. Toxigenic Fusaria. Mycotoxins and Animal Foods. Ed. by J.E. Smith and R.S. Henderson. USA. CRC Press, pp: 119-139.
- Maria, L., M. Marina and B. Fernando, 2003. Fungal lfora and mycotoxins detection in commercial pet food, Lisboa, Portugal., 98: 179-183.
- Martins, M.L. and H.M. Martins, 1999. Natural and in vitro co-production of cyclopiazonic acid and aflatoxins. J. Food Protec., 62: 292-294.
- Meireles, M.C.A.,B. Correa, O. Fischman, W. Gambale, C.R. Paula, NO. Chacon-Reche and C.R. Pozzi, 1994. Mycoflora of the toxic feeds ssociated with equine leokoencephalomalacia (ELEM) outbreaks in Brazil. Mycopathologia, 127: 183-188.
- Misra, J.K., E.B. Gergon and T.W.Mew, 1995. Storage fungi of rice in the Phillipines. Mycopathologia, 13(1): 13-24.
- Nyamongo, J. and M. Okioma, 2005. The aflatoxin outbreaks in Kenya in 2004 and 2005: a case study. In: Reducing impact of Mycotoxins in Tropical Agriculture with emphasis on Health and Trade in Africa, Accra, Ghana, pp: 13-16.
- Ogbulie, J.N., 1995. Microbial ecology of tropical aquatic system. Ph. D thesis. University of Port- Harcourt Nigeria.
- Opera, M.N. and I.C. Okoli, 2005. Strategies for reduction of mycotoxm contaminations in Animal productions panacea for the problems in Southeastern Nigeria. In: Reducing impact of Mycotoxins in Tropical Agriculture with emphasis on Health and Trade in Africa. Accra, Ghana, pp: 13-16.
- Osho, LB., T.A.M. Awoniyi and A.I. Adebayo, 2007. Mycological investigation of compounded poultry feeds used in poultry farms in southwest Nigeria, African J. Biotechnol., 6: 1833-1836.
- Pattron, D.D., 2006. Aspergillus, Health implication and recommendations for public health food safety. Internet J. Food Safety, 8: 19-23.
- Pitt, J.I., A.D. Hocking, B. Kanjana, B.F. Miseamble, K.A. Wheeler and P. Tanboon, 1994. The normal mycoflora of commodities form Thailand Rice, Beans and other commodities. International J. Environmental Health Res., 4: 102-108.
- Pitt, J.I. and A.D. Hocking, 1997. Fungi and Food Spoilage (2"d ed.). London: Black Academic Press.
- Raper, K.D. and D.I. Fennel, 1965. The genus Aspergillus. Bultimore (edn). The Williams and Wilkins Co., pp: 357-404.
- Rippon, J.W., 1988. Medical mycology. 2"d ed. W.B. Saunders Company, Chicago,Illinois, USA, pp: 640-641.
- Rosa, C.A.R., V. Palacios, M. Combina, M.E. Fraga, A.D. Rekson and A.M. Dalcero, 2002. Potential ochratoxin A producers from wine grapes in argentina and Brazil. Food Addit. Contain, 19: 408-414.
- Sefer, D., N. Jovanovic, R. Markovic, D. Kmjajic, J. Nedeljkovic-Trailovic and Z. Sinovec, 1998. Pregled kvaliteta krmnih smesa za ishranu ivine. Vet Glasnik, 52 (7/8): 425-435.
- Summerell, B.A., J.F. Leslie, D. Backhouse, W.L. Bryden and W. Burgess, 2001. Fusarium, Paul E. Nelson Memorial Symposium(APS Press, St Paul, MN).
- Sweeney,M.J. and A.D.W. Dobson,1998. Mycotoxm production by Aspergillus, Fusarium and Penicillium species. Intern J. Food Microbiol., 43: 141-158.
- Trenholm, H.L., L.L. Charmley and L. PreLusky, 2000. Mycotoxin binding agents: An Update. Farming Today, 1: 11.
- Zimmerli, B. and R. Dick, 1996. Ochratoxin A in table wines and grape juices: occurrence and risk assessment. Food Addit. Contain, 13: 655-668.