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Upasani, Chandrashekhar D.
- Analgesic and Antiinflammatory Activity of Amarwel Extracts on Experimentally Induce Pain and Inflammation on Animals
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Materials and Methods: Petroleum ether extract, methanol extract, and aqueous extracts of Cuscuta reflexa (PECR, MECR, and AECR respectively) at three dose levels of 50, 200 and300 mg/kg body weight of an animal by oral route were used for biological activities. The analgesic activity of was determined using hot plate analgesia, acetic acidinduced writhing response and formalin test. The anti-inflammatory activity of was determined using models like carrageenan, serotonin and histamine-induced paw edema models along with cotton pellet induced granuloma. Probable mechanism involved in the antiinflammatory effect of methanol and aqueous extracts was evaluated with various tests like ulcerogenicity test; acetic acid induced vascular permeability test, and leukocyte migration test using a single dose of 300 mg/kg orally.
Results: The HPTLC analysis showed presence of quercetin in MECR and AECR (0.121 and 0.071 mg% respectively). Acute oral toxicity test revealed the LD50 of >2 g/kg. PECR, MECR and AECR (200 and 300 mg/kg p.o.) significantly (P<0.05) increased latency against thermal stimulus, decreased the acetic acid-induced writhing responses and licking times of the second phase in the formalin test. Moreover, MECR and AECR (200 and 300 mg/kg p.o.) exhibited significant (P<0.01) antiinflammatory effect against carrageenan and mediator-induced paw edema.
Conclusion: Results suggest significant analgesic and anti-inflammatory effects produced by MECR and AECR.
Authors
Affiliations
1 KCTS R. G. Sapkal College of Pharmacy, A/p-Anjaneri, Tal-Trimbakeshwar, Dist-Nashik, Maharashtra, IN
2 Bhujbal Knowledge City, MET's Institute of Pharmacy, Adgaon, Nashik, Maharashtra, IN
3 KCT'S RGS College of Pharmacy, Anjaneri, Nashik, Maharashtra, IN
4 SNJB's SSDJ College of Pharmacy, Neminagar, Chandwad, Nashik, Maharashtra, IN
1 KCTS R. G. Sapkal College of Pharmacy, A/p-Anjaneri, Tal-Trimbakeshwar, Dist-Nashik, Maharashtra, IN
2 Bhujbal Knowledge City, MET's Institute of Pharmacy, Adgaon, Nashik, Maharashtra, IN
3 KCT'S RGS College of Pharmacy, Anjaneri, Nashik, Maharashtra, IN
4 SNJB's SSDJ College of Pharmacy, Neminagar, Chandwad, Nashik, Maharashtra, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 6, No 2 (2014), Pagination: 112-117Abstract
Objective: To study Analgesic and anti-inflammatory activity of Amarwel extracts on experimentally induce pain and inflammation on animalsMaterials and Methods: Petroleum ether extract, methanol extract, and aqueous extracts of Cuscuta reflexa (PECR, MECR, and AECR respectively) at three dose levels of 50, 200 and300 mg/kg body weight of an animal by oral route were used for biological activities. The analgesic activity of was determined using hot plate analgesia, acetic acidinduced writhing response and formalin test. The anti-inflammatory activity of was determined using models like carrageenan, serotonin and histamine-induced paw edema models along with cotton pellet induced granuloma. Probable mechanism involved in the antiinflammatory effect of methanol and aqueous extracts was evaluated with various tests like ulcerogenicity test; acetic acid induced vascular permeability test, and leukocyte migration test using a single dose of 300 mg/kg orally.
Results: The HPTLC analysis showed presence of quercetin in MECR and AECR (0.121 and 0.071 mg% respectively). Acute oral toxicity test revealed the LD50 of >2 g/kg. PECR, MECR and AECR (200 and 300 mg/kg p.o.) significantly (P<0.05) increased latency against thermal stimulus, decreased the acetic acid-induced writhing responses and licking times of the second phase in the formalin test. Moreover, MECR and AECR (200 and 300 mg/kg p.o.) exhibited significant (P<0.01) antiinflammatory effect against carrageenan and mediator-induced paw edema.
Conclusion: Results suggest significant analgesic and anti-inflammatory effects produced by MECR and AECR.
Keywords
Dodder, Hot Plate, Writhing, Formalin Test, Carrageenan, Granuloma, Quercetin.
References
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