A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Firdous, Ansari
- Pharmacodynamic Drug Interaction of Ethionamide with Glibenclamide in Normal and Diabetic Rats
Authors
1 Department of Pharmacology, HKE’s Matoshree Taradevi Rampure Institute of Pharmaceutical Sciences, Sedam Road, Gulbarga-585105, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 5, No 4 (2013), Pagination: 227-231Abstract
The present study was aimed to find out the effect of treatment of ethionamide, an antitubercular drug on hypoglycaemic activity of glibenclamide in normal and diabetic rats. The study was intended to determine the pharmacodynamic parameters of drug interaction between glibenclamide and ethionamide in normal and diabetic rats. The studies were conducted using six group of normal adult rats of either sex. They were treated with half therapeutic dose of ethionamide (0.18 mg/200 g), therapeutic dose of ethionamide (0.36 mg/200 g), double therapeutic dose of ethionamide (0.72 mg/200 g), therapeutic dose of glibenclamide (0.18 mg/200 g) and combination of therapeutic dose of ethionamide and glibenclamide (0.36 mg/200 g + 0.18 mg/200 g).
Another group of six rats were taken and diabetes was induced by administering alloxan at a dose of 100 mg/ kg body weight intraperitoneally. Rats with glucose levels more than 200 mg/dL were considered for studied.
The blood samples were collected from tail vein at predetermined time intervals and blood glucose level was estimated using GOD/POD method with the aid of ARTOS semi auto analyser. Ethionamide produced hypoglycaemia when administered alone. The results indicated that in both normal as well as in diabetic rats ethionamide treatment altered the hypoglycaemic activity when administerd along with glibenclamide. This may be due to the synergistic effect of ethionamide with glibenclamide. The preliminary study indicate the combination may be unsafe in diabetes associated with tuberculosis.
Keywords
Glibenclamide, Ethionamide, Drug Interaction, GOD/POD Method, RatsReferences
- Shorvon SD, Reynolds EH. Unnecessary polypharmacy for epilepsy. Br. Med J 197; 1635-37.
- Steel KK, Gertman PM, Gresienze C, Andersen J. Iatrogenic illness on a general medical service at a university hospital. N Eng J Med 1981; 34: 638-42.
- King H, Aubert KE, Herman WH. Global burden of diabetes 1995-2025. Prevalence, numerical estimate and protection. Diabetes care 1998; 2: 1414-31.
- Songer TJ, Zimmet PZ. Epidemology of type II diabetes on international perspective, pharmacomeyrics 8 (supl.): 1995; 1- 11.
- World Health Organization. Global tuberculosis control: 2010 Geneva: World Health Organization; 2010.
- Rang HP, Dale MM, Ritter JM, Moore PK. Pharmacology. 5th Ed. Edinburg: Churchill Livingstone; 2003.
- Patel JC. Complications in 8793 cases of diabetes mellitus 14 years study in Bombay hospital, India. Ind I. Med. Sci. 1989, 43, 177.
- Ezung T, Devi NT, Singh TB. Pulmonary tuberculosis and diabetes mellitus- a study. J. of Indian Medical Association. 2002, 100, 316.
- Goswami R, Kochupillai N. Endocrine implication of tuberculosis. In: Sharma SK, Mohan A (Eds). Tuberculosis. New Delhi: Jaypee Brothers Medical publishers (P) Ltd., 2001, 386.
- Mboussa J, Monabeka H, Kombo M, Yokolo D, Yoka- Mbio A, Yala F. Course of tuberculosis in diabetics. Rev. pneumol clin. 2003, 359, 36.
- Bhatia JL, Lal H. (1966). Indian J. Tnberc. 13, 57.
- Laurence DR, Bacharach AL. Evaluation of drug activities and pharmacometrics. London and New York: Academic Press; 1964.
- Trinder P. Ann Clin Biochem 1964; 6: 24.
- Goldhaber-Fiebert JD, Jeon CY, Cohen T, et al. Diabetes mellitus and tuberculosis in countries with high tuberculosis burdens: individual risks and social determinants. Int J Epidemiol 2011; 40 (2): 417-428.
- World Health Organization. Tuberculosis and diabetes. Collaborative Framework for care and control of tuberculosis and diabetes. WHO sept. 2011. www.who.int/td.
- Antimicrobial Activity of Root Extracts of Cyperus rotundus (Linn) using Diarrhoea Inducing Microbes
Authors
1 Department of Pharmacology, HKE’S Matoshree Taradevi Rampure Institute of Pharmaceutical Sciences Gulbarga, Sedam Road, Gulbarga-585105, Karnataka, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 5, No 4 (2013), Pagination: 244-246Abstract
Due to the development of antibiotic resistance and the outbreak of infectious diseases caused by resistant pathogenic bacteria, the pharmaceutical companies and the researchers are now searching for new unconventional antibacterial agents. Hence, there is an ever-growing need to develop new antimicrobial compounds. In the present study various medicinal plants were selected to check the antimicrobial activity and Cyperus rotundus (linn) was found to have the best activity against microbes. Pet ether extract, chloroform extract and methanol extract were screened for their antimicrobial activity against various microbes which are responsible for inducing diarrhoea viz. Staphylococcus aureus, Bacillus cereus, Escherichia coli and Campylobacter jejunir using agar well diffusion method. Methanolic extract at the dose of 100 μg, have shown good antibacterial activity where as Methanolic extract at the dose of 80μg have shown moderate activity, using Loperamide as a standarad drug, against Staphylococcus aureus, Bacillus cereus, Escherichia coli and Campylobacter jejuni with zones of inhibition ranging from 19 mm to 24 mm. It might be concluded from the present study that the potential of this herb to produce useful antimicrobial compounds is great and must be better exploredKeywords
Cyperus rotundus (linn), Pet Ether, Chloroform and Methanolic Extract Loperamide Antimicrobial ActivityReferences
- Sharma PC, Yelne MB, Dennis TJ, editors. Database on medicinal plants used in ayurveda. Vol 2.
- New Delhi: Central Council for Research in Ayurveda and Siddha; 2001. p. 481-9. http://www.wikipedia.com//Cyperus rotundus .5http:// www . google. com// plantkingdom. Com /Cyoerus rotundus. Cyperus rotundus.Usambara Invasive plants. Tropical Biology Association Retrieved2008;03:25http://www.tropicalbiology.org/research/di p/species/Cyperus%20rotundus.htm
- Rakotonirina V S, Bum EN, Rakotonirina N, Bopelet M. Sedative properties of the decoction of the rhizome of Cyperus articulatus. Fitoterapia 2001; 72:
- Cruickshank R, Duguid JP, Marmion BP, Swain RHA, editors. Medical Microbiology. Great Britain: Longman Group Ltd; 1975.
- Galvez J, Zarzuelo A, Crespo ME. Antidiarrhoeal activity of Euphorbia bitra extract and isolation of an active flavonoids constituent. Planta Med 1993. p. 333-6.
- Carrol KC, Reimer L. Infectious diarrhea: Pathogens and treatment. Leb Med J 2000; 48:2707.
- World Health Organization (WHO). The World Health Report. Geneva: WHO; 2004.
- Ganguly NK, Kaur T. Mechanism of action of cholera toxin and other toxins. Indian J Med Res 1996; 104:28 37.
- Gopal Krishna Rao, Rajasekaran S and Sanjay PN. Synthesis and antimicrobial activity of some 2-phenyl-1-{4-phenyl-1,3- thiazol-2-yl}-4-(substituted benzylidene)–imidazoline-5-ones. Indian Journal of Heterocyclic Chemistry Jan-March 2010; 19:303-304.
- Petri plates containing Int. J. Trad. Nat. Med. 2012, 1(2): 83-91
- Carrol KC, Reimer L. Infectious diarrhea: Pathogens and treatment. Leb Med J 2000; 48:270-7.
- Pazhani GP, Subramanian N, Arunchalam G, Hemalatha S, Ravichandran V. Antidiarrhoeal potential of Elephantopus scaber Linn leaf extract. Indian Drugs 2001; 38(5): 269-71
- Ezekwesili CN, Obiora KA, Ugwu OP. Evaluation of Anti- Diarrhoeal Property of Crude Aqueous Extract of Ocimum gratissimum L.(Labiatae) In Rats. Biokemistr 2004; 16(2): 122-131.
- Lagos R, Fasano A, Wasserman SS, Prado V, Martin OS, Abrego P, et al . Effect of small bowel bacterial overgrowth on the immunogenicity of single-dose live cholera vaccine CVD 103-HgR. J Inf Dis 1999;180:170