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Begum, Amreen
- Pharmacodynamic Drug Interaction of Ethionamide with Glibenclamide in Normal and Diabetic Rats
Authors
1 Department of Pharmacology, HKE’s Matoshree Taradevi Rampure Institute of Pharmaceutical Sciences, Sedam Road, Gulbarga-585105, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 5, No 4 (2013), Pagination: 227-231Abstract
The present study was aimed to find out the effect of treatment of ethionamide, an antitubercular drug on hypoglycaemic activity of glibenclamide in normal and diabetic rats. The study was intended to determine the pharmacodynamic parameters of drug interaction between glibenclamide and ethionamide in normal and diabetic rats. The studies were conducted using six group of normal adult rats of either sex. They were treated with half therapeutic dose of ethionamide (0.18 mg/200 g), therapeutic dose of ethionamide (0.36 mg/200 g), double therapeutic dose of ethionamide (0.72 mg/200 g), therapeutic dose of glibenclamide (0.18 mg/200 g) and combination of therapeutic dose of ethionamide and glibenclamide (0.36 mg/200 g + 0.18 mg/200 g).
Another group of six rats were taken and diabetes was induced by administering alloxan at a dose of 100 mg/ kg body weight intraperitoneally. Rats with glucose levels more than 200 mg/dL were considered for studied.
The blood samples were collected from tail vein at predetermined time intervals and blood glucose level was estimated using GOD/POD method with the aid of ARTOS semi auto analyser. Ethionamide produced hypoglycaemia when administered alone. The results indicated that in both normal as well as in diabetic rats ethionamide treatment altered the hypoglycaemic activity when administerd along with glibenclamide. This may be due to the synergistic effect of ethionamide with glibenclamide. The preliminary study indicate the combination may be unsafe in diabetes associated with tuberculosis.
Keywords
Glibenclamide, Ethionamide, Drug Interaction, GOD/POD Method, Rats
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- Antimicrobial Activity of Root Extracts of Cyperus rotundus (Linn) using Diarrhoea Inducing Microbes
Authors
1 Department of Pharmacology, HKE’S Matoshree Taradevi Rampure Institute of Pharmaceutical Sciences Gulbarga, Sedam Road, Gulbarga-585105, Karnataka, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 5, No 4 (2013), Pagination: 244-246Abstract
Due to the development of antibiotic resistance and the outbreak of infectious diseases caused by resistant pathogenic bacteria, the pharmaceutical companies and the researchers are now searching for new unconventional antibacterial agents. Hence, there is an ever-growing need to develop new antimicrobial compounds. In the present study various medicinal plants were selected to check the antimicrobial activity and Cyperus rotundus (linn) was found to have the best activity against microbes. Pet ether extract, chloroform extract and methanol extract were screened for their antimicrobial activity against various microbes which are responsible for inducing diarrhoea viz. Staphylococcus aureus, Bacillus cereus, Escherichia coli and Campylobacter jejunir using agar well diffusion method. Methanolic extract at the dose of 100 μg, have shown good antibacterial activity where as Methanolic extract at the dose of 80μg have shown moderate activity, using Loperamide as a standarad drug, against Staphylococcus aureus, Bacillus cereus, Escherichia coli and Campylobacter jejuni with zones of inhibition ranging from 19 mm to 24 mm. It might be concluded from the present study that the potential of this herb to produce useful antimicrobial compounds is great and must be better exploredKeywords
Cyperus rotundus (linn), Pet Ether, Chloroform and Methanolic Extract Loperamide Antimicrobial ActivityReferences
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