Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Antinociceptive Activity of Tulsi Amrit (A Polyherbal Formulation) In Selective Pain Induced Models in Rats


Affiliations
1 Dept. of Pharmacology, HKES-MTRIPS, Kalaburgi, India
     

   Subscribe/Renew Journal


Ocimum sanctum commonly known as Tulsi has been used for thousands of year in the ayurveda for its diverse healing properties. This study was conducted to evaluate the antinociceptive activity of tulsi oil (Tulsi Amrit) in pain induced rat models . The rats were divided into 4 Groups of 6 animals each (n=6). Group 1 – served as control, Group 2- was treated with standard drug piroxicam and Group 3 an Group 4 – were treated with test substance at two dose levels. The models selected to study the effect on nociception were hot plat assay, cold plate test in rats. The tusi oil demonstrated significant ( P < 0.0001) antinociceptive activity at the doses (100 and 200mg/kg body weight P.O) tested compared to standard piroxicam 20mg/kg. The tulsi oil (Tulsi amrit) was found to have a significant ( P < 0.0001) inhibitory effect on hot plate at both the doses as well on cold plate. The study reveals antinociceptive activity of Tulsi Amrit.

Keywords

Tulsi Oil, Antinociceptive, Piroxicam, Hot Plate, Cold Plate.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Calixto JB, Campos MM, Santos ARS. Botanical analgesic and anti-inflammatory drugs. J ethanopharmacology.2007;2.
  • Mrtienz MJ, Betancourt J, Alonso-gonzalez N, Jauregui AJ. Screening of some Cuban medicinal plants for antimicrobial activity. J ethanopharmacol.1996; 52 (3): 171-74.
  • Calixto JB, Beirith A, Ferreira J et.al. Naturally occurring antinociceptive substance from plants. Phytother Res. 2000; 14 (6): 401-18.
  • Loeser JD, Melzack R. Pain : an overview. The lancet. 1999; 353 (9164): 1607-09.
  • Woolf CJ, Mannion RJ. Neuropathic pain : A Etiology symptoms mechanism and management. The lancet. 1999; 353 (9168): 1959-64.
  • Treed RP, Jansen TS, Campbell J et.al. Neuropathic pain redefinition and grading system for clinical and research purpose. Neurology. 2008; 70 (18): 1630-35.
  • Axelrod FB, Hilz MJ. “ Inherited autonomic neuropathies”. Semin neurol.2003; 23(4): 381-90.
  • Bishop GH, Landau WM, et.al. Evidence for a double peripheral pathway for pain. J Science. 1358; 128(3326): 721-4.
  • Gurejeo, von korff M, kola l et.al. The relationship between multiple pains and mental disorders: results from the mental health surveys. Pain 2008;135: 82-91.
  • Shinde UA, Phadake AS, Nair AM, Nair AM et al. Studies on the anti-inflammatory and analgesic activity of Cedrus deodara (Roxb.) loud wood oil. J Ethanopharmacol. 1999; 65(1): 21-7.
  • Jasmin L, Kohan L, Franssen M, Janni G, Goff J. The cold plate as a test of nociceptive behaviours: description and application to the study of chronic neuropathic and inflammatory pain models. Pain 1998; 75: 367-82.
  • Vissers K, Adriaensen H, De coster R, De deyne C, Meert TF. A chronic constriction injury of the sciatic nerve reduces bilaterally the responsiveness to formalin in rats: a behavioural and hormonal evaluation. Anesthesia and Analgesia 2003; 97(2): 520525.
  • Derardt R, Jougney S, Delevalcee F, Falhourt M. Release of prostaglandins E and F in an algogenic reaction and its inhibition. Eur. J. Pharmacol. 1980; 51:17-24.
  • Ahmadiani A, Fereidoni M, Semnanian S, Kamalinejad M, Sremi S. Antinociceptive and anti-inflammatory effect of Sambucus ebulus rhizome extract in rats.

Abstract Views: 440

PDF Views: 1




  • Antinociceptive Activity of Tulsi Amrit (A Polyherbal Formulation) In Selective Pain Induced Models in Rats

Abstract Views: 440  |  PDF Views: 1

Authors

P. Basavraj
Dept. of Pharmacology, HKES-MTRIPS, Kalaburgi, India
M. Nitin
Dept. of Pharmacology, HKES-MTRIPS, Kalaburgi, India

Abstract


Ocimum sanctum commonly known as Tulsi has been used for thousands of year in the ayurveda for its diverse healing properties. This study was conducted to evaluate the antinociceptive activity of tulsi oil (Tulsi Amrit) in pain induced rat models . The rats were divided into 4 Groups of 6 animals each (n=6). Group 1 – served as control, Group 2- was treated with standard drug piroxicam and Group 3 an Group 4 – were treated with test substance at two dose levels. The models selected to study the effect on nociception were hot plat assay, cold plate test in rats. The tusi oil demonstrated significant ( P < 0.0001) antinociceptive activity at the doses (100 and 200mg/kg body weight P.O) tested compared to standard piroxicam 20mg/kg. The tulsi oil (Tulsi amrit) was found to have a significant ( P < 0.0001) inhibitory effect on hot plate at both the doses as well on cold plate. The study reveals antinociceptive activity of Tulsi Amrit.

Keywords


Tulsi Oil, Antinociceptive, Piroxicam, Hot Plate, Cold Plate.

References